A student makes a new gel using agarose, water, and buffer solution. The student loads their DNA samples in their wells and places the gel in the chamber for an appropriate length of time. When the student places the gel on the UV lightbox, no DNA bands show up at all. Even the marker lane is clear and has no bands! What mistake did the student make? Explain your answer.

Answers

Answer 1

Answer:

A dye needs to be added to the gel electrophoresis

Explanation:

Ethidium bromide (EtBr) is a reagent that adheres to DNA and fluoresces under ultraviolet (UV) light. Due to this feature, EtBr is widely used to dye DNA strands in molecular biology techniques such as agarose gel electrophoresis. The most common dyes for visualizing and staining nucleic acids during gel electrophoresis include EtBr, SYBR gold, Eva green and SYBR safe. If EtBr (or any other staining agent) was not added to the electrophoresis gel and/or running buffer, the gel will be dark under UV light (even the ladder lane will look empty) because DNA has not been stained.

Answer 2

The mistake the student made was by not adding dye to the sample. Dyes

are commonly used during electrophoresis.

Agarose gel electrophoresis is the process which helps in the separation

and purification of DNA samples. This process involves the use of dyes

which acts as markers.

in this scenario, we were told no DNA bands show up and the marker lane is

clear means no dye was added.  The common dye used in agarose gel

electrophoresis is Ethidium bromide (EtBr) which should be added by the

student.

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Related Questions

In pea plants, yellow seeds are dominant over green seeds. What would be the
expected genotypic ratio in a cross between a plant with green seeds & a plant that
is heterozygous for yellow seed color?
A. 1:1
B. 1:3
C. 1:2:1
D. 4:1

Answers

The expected genotype ratio is 1:3.

The genotype of an organism is the sum total of the gens that the organism received from its parents. The genotype determines the traits of an organism (phenotype).

Let the dominant gene be R and the recessive gene be r then we have a cross between RR and Rr. The expected genotype ratio is 1:3.

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Which statement best describes members of the same species?
O They possess identical numbers of cells in their bodies
O They possess identical traits and physical appearances
O They are able to breed with each other and produce offspring of different species
They are able to breed with each other and produce offspring of the same species

Answers

Answer: D.

Explanation: Members of the same species do not have the same number of cells. Think differences in height or weight. They do not have identical traits. Think siblings. They cannot create different species. Two wolves cannot make a coyote.

Mitosis creates how many daughter cells

Answers

I believe they create 7

After food is made in the form of glucose in the leaf, it is then converted to
for transport, then to
for storage.

A) glucose, starch
B) glucose, sucrose
C) sucrose, starch
D) glucose, sugars

Answers

I think A I’m sorry if I’m wrong

Why do we cook the traditional Chinese dish "pork knuckles and ginger stew" in a clay pot rather than a metal pot?

Answers

Use a large clay pot or a casserole (Don’t use cast iron or metal ones though, not suitable for cooking vinegar.)

pls help! on gregor mendel

Answers

Answer:

P, F₁, and F₂

Explanation:

The parental generation is also known as P.  F₁ is the first generation and F₂ is the second generation.  

Examples of obligate intracellular parasites that can only replicate inside a host cell are:
viruses and prions
rickettsia and viruses
viruses and bacteria
rickettsia and prions

Answers

Answer:

rickettsia and viruses

Explanation:

This is because obligate intracellular parasite are parasite that affect humans and they grow, reproduce and survive inside the host cell. These parasite cannot live or survive outside host cell but they live inside host cell, replicate and they depend on the host cell resources i.e the use host cell resources thereby causing harm on the host cell. rickettsia and viruses are parasite that can only survive inside a living organism.

Answer:

rickettsia and viruses

Explanation:


Explain How might
chemical weathering
change the appearance
of this obelisk?

Answers

it makes it look old an wears it down.

Describe how the process of apoptosis occurs?

Answers

Answer:

Apoptosis is an orderly process in which the cell's contents break down and are packaged into small packets of membrane for “garbage collection” by immune cells. It contrasts with necrosis (death by injury), in which the dying cell's contents spill out and cause inflammation. Apoptosis removes cells during development.

Apoptosis is a form of programmed cell death that occurs in multicellular organisms.

What is apoptosis ?

It is a  natural biological process of programmed cell death in which the cells destroy themselves and maintain the smooth function of the body parts.

There are two types cell death such as Programmed death of cells or apoptosis and uncontrolled death or Necrosis.

Apoptosis can occur via two pathway such as Extrinsic Pathway is triggered in response to external stimuli, ligand binding at death receptor and these receptors are called as Tumor Necrosis Factor gene.

Intrinsic Pathway triggered in response to internal stimuli such as biochemical stress, DNA damage and this pathway is modulated  Bax, and Bcl-2 molecules.

Apoptosis helps in maintaining homeostasis, proper size of the body, cell number, elimination of unwanted cell, T-lymphocytes etc.

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How does hair help prevent diseases?

Answers

Hair plays an important role in our lives. The hair on our head keeps us warm and acts as a cushion for our skull. Hair appearance can also help form our identity and self-image, which makes hair loss very troubling.

Most hair disorders aren’t serious, but they are often considered major cosmetic issues that require treatment. Androgenetic alopecia is the most common form of hair loss, although there are a number of other hair conditions that can affect the scalp and other parts of the body.

what substances form the uprights of the DNA ladder

Answers

DNA ladder substances the x and y

Can you explain why DNA must be replicated before mitosis and the role of helicase in DNA replication

Answers

Answer:

1. dna needs to be replicated before mitosis so that the parent cell has the same amount of genetic material as the parent cell once it has split.

2. helicase is an enzyme that is so dna can unwind.

Explanation:

the explanation is in the answer.

I hope this helps!

Answer:

DNA must be replicated before mitosis so that both identical daughter cells contain the same amount of genetic material as the parent cell once it has split in two. Helicase is an enzyme necessary at the initiation of DNA replication to unwind/unzip the DNA so that this process may begin.

Viruses cannot live on their own, and instead need to infect a/n _______ to replicate.

Answers

Answer:

Host Cell

Explanation:

Viruses cannot live on their own, and instead need to infect a/n Host Cell to replicate. The two primary life cycles of viruses are the lytic cycle, also known as the virulent cycle, and the Lysogenic cycle, also known as the temperate cycle.

Answer:

host cell

Explanation:

How does dew form on grass?
O A. It is formed from melted frost.
O B. It is pushed out of holes in the grass.
C. It is formed on the grass from water in the air.
D. It falls as melted snow and is caught on the grass.
E. it falls like a light rain and is caught on the grass.

Answers

Answer:

Dewy Grass. Dew is a natural form of water, formed as water vapor condenses. ... This forces water vapor in the air around cooling objects to condense. When condensation happens, small water droplets form—dew.

so it's C

Explanation:

Each category represents one of the functions of proteins. Match each example with the appropriate function.
fighting disease
Insulin controls the concentration of sugar in
the blood.
regulating chemical reactions
IgA prevents the buildup of viruses and
bacteria in the gut.
providing structure
Collagen makes skin strong yet flexible.
maintaining homeostasis
Pepsin works in the stomach to speed up the
breakdown of food.

Answers

Answer:

1. Providing structure --> Collagen makes skin strong yet flexible.

2. Maintaining homeostasis --> Insulin controls the concentration of sugar in the blood.

3. Fighting Disease --> IgA prevents the buildup of viruses and bacteria in the gut.

4. regulating chemical reactions --> Pepsin works in the stomach to speed up the breakdown of food.

How does thermal energy affect the earth's layers?

Answers

Answer:

The thermal energy of the Earth remains from its formation and is continuously generated from radioactive decay of long-lived radionuclides in the Earths interior. The deeper the Earth layers the higher the temperature with the geothermal gradient of temperatures through the Earth’s crust on average as high as 25–30°C/km.

Explanation:

The thermal energy affect the earth's layers by forming a thermal gradient.

Thermal energy of the Earth:

Deep inside the earth the thermal energy is generated by the radioactive decay of long-lived radionuclides.

This creates a thermal gradient from core to surface of the earth. The temperature of the core is highest, then the manle is semi-solid or liquid.

The crust of the earth is solid and have relatively less temperature,

Therefore, the thermal energy affect the earth's layers by forming a thermal gradient.

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What are some of the WORST things about coal fuel?



help me please help me​

Answers

Answer:

Explanation:

The burning of fossil fuels releases greenhouse gases into the atmosphere, increasing levels of CO2 and other gasses, trapping heat, and contributing to global climate change. Coal combustion releases the greenhouse gases carbon dioxide (CO2) and nitrous oxide (N2O) during combustion. The major disadvantage of coal is its negative impact on the environment. Coal-burning energy plants are a major source of air pollution and greenhouse gas emissions. In addition to carbon monoxide and heavy metals like mercury, the use of coal releases sulfur dioxide, a harmful substance linked to acid rain.

Answer:

Air pollution

Can impact health of citizens like asthma, breathing difficulties, etc.

Can lead to death if not treated or fixed.

Releases greenhouse gases into the atmosphere.

Contributes to global climate change.

How are rivers made ?

Answers

Answer:

Rivers usually begin in upland areas, when rain falls on high ground and begins to flow downhill. They always flow downhill because of gravity. They then flow across the land - meandering - or going around objects such as hills or large rocks.

Explanation:

Hope this helps!

Answer:

A river is created when water moves from higher elevation to lower elevation. It usually goes downhill, and can join with other rivers. The river ends when it flows into an ocean.

I hope this helps!

Police are investigating a series of murders in which the suspect is leaving little to no physical evidence, such as fingerprints or DNA. They cannot even establish an obvious link between victims. The only real evidence they have are strange pieces of typewritten poetry found tucked into the victims’ pockets. Who might the police call on to give valuable insight to help them identify the perpetrator and possible future victims? Forensic anthropologist Forensic entomologist Forensic pathologist Forensic psychiatrist

Answers

Answer:

Forensic anthropologist

Explanation:

Forensics is a branch of science that deals with the solving of crimes. According to this question, police are investigating a series of murders in which the suspect is leaving little to no physical evidence such as DNA or fingerprint. However, strange pieces of typewritten poetry was found tucked into the victims’ pockets as evidence.

Based on this evidence found, a FORENSIC ANTHROPOLOGIST is the person that can give valuable insight to help the police identify the perpetrator and possible future victims. This is because ANTHROPOLOGY is a social science subject that deals with past events, hence, forensic anthropologists will be able to work on the typewritten poetry.

Question 24 of 35
Which two statements describe the Mesozoic era?
A. Most of Earth's history took place within this time.
B. Plants began to colonize land during this era.
✓ C. It is also known as the Age of Reptiles.
D. It lasted from about 250 million years ago to 70 million years ago.

Answers

I’ve done this awhile back I’m pretty sure it’s C and D

Answer:

C

Explanation:

it was the time to f the dinosaurs and some of the dinosaurs were reptiles

Plants are known as autotrophs
and make their own food. What
do we call organisms that make
their own food?
A
Primary producer
B
Tertiary consumer

Answers

A. primary producers are plants

Consumers do not make their own food.
A because they can make there own food while B can’t

The members of the phylum Porifera are commonly called?

Answers

Answer:

Sponges

Explanation:

Members of phylum Porifera are commonly known as sponges. They are generally marine and mostly asymmetrical animals. Spongilla is a fresh water sponge.

From Mendel's first set of experiments, he concluded that
a
there are two factors controlling each characteristic
he studied.
b the two factors separate and go to different
gametes.
с
of the two factors, one of the factors dominates the
other factor.
d all of the above are correct

Answers

Answer:

d. all of the above are correct.

When we doing strawberry extraction,why do we have to remove the air as much as possible before we seal the bag?

Answers

Answer:

Explanation:

Oxygen is needed to support life and some metabolic processes in microorganisms especially aerobic organism.

The presence of oxygen in the container gives them the opportunity to carry out there metabolic activities thereby enhancing there growth and multiplication.

Hence, to limit the growth of aerobic bacteria or fungi, and also to prevent the evaporation of volatile components that is available in the content oxygen is withdrawn this help extend the shelf life of the produce to be stored.

Help me pls hajkskwksmsmsmskwmsksmsss

Answers

Answer:

The easiest one to correct would most likely be Class V, since in this type of mutation, the only problem or abnormality is the production of insufficient amounts of the needed CFTR.

Explanation:

Mutation Classes

Class I - Gene contains a stop signal that prevents CFTR from being made.

Class II - CFTR is made, but does not reach the cell membrane

Class III - CFTR is made and in the right place, but does not function normally

Class IV - Channel does not move substances efficiently or at all

Class V - CFTR is made in smaller than normal quantities

in which of these is osmosis occurring
1 plant is absorbing water from the soil
2sugar is being taken up into the blood from the gut
3 water is evaporating from a leaf
4oxygen is entering the blood from the lungs

Answers

Answer:

1.Plant absorbing water from the soil.

Explanation:

Osmomsis occurs by the movement of a water from a region of higher concentration of water molecules to a region of lower concentration of water molecules, through a semi permeable membrane,

OR

It occurs as a result of movement of substance molecules from a region of lower concentration to a region of Higher concentration of the substance.

Note: The higher the concentration of the substance molecules,the lower the concentration of water molecules.

The movement of water into the roots of the plants from the soil demonstrates the most valid and accurate example of osmosis. Thus, the correct option for this question is A.

What is Osmosis?

Osmosis may be defined as a type of process through which the movement of a solvent such as water takes place from a region of lower solute concentration to a region of higher solute concentration through a semi-permeable membrane.

The absorption of sugar into the blood from the gut is mediated by secondary active transport with the help of transporters like SGLT-1 and SGLT-2. The process through which water is evaporating from a leaf is known as transpiration. The process of entering oxygen into the blood from the lungs is known as diffusion.

Therefore, the movement of water into the roots of the plants from the soil demonstrates the most valid and accurate example of osmosis. Thus, the correct option for this question is A.

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all organic molecules contain what elements?

Answers

Answer: All organic compounds contain the elements carbon and hydrogen.

PLEASE MARK ME BRAINLIEST

Explanation:

Answer:

All organic compounds contain the elements carbon and hydrogen

Explanation:

Gene segments may initiate, terminate, or code for a particular amino acid sequence.

A Gene Segment: A G C T A G

The number of amino acids coded for by the gene segment shown above is:

Answers

I think 2 amino acids...

7. An animal that eats plants is called
a(n)
A) food chain
B) omnivore
C) food web
D) herbivore​

Answers

Answer:

herbivore

Explanation:

the word herbivore contains the word "herb" meaning plants

Cyanobacteria are important to the evolution and advancements of other life on Earth because they

options:

were the first lifeform on earth.

produced oxygen from photosynthesis.

were thought to have become mitochondria in the cells of animals.

were the first group of organisms to leave a fossil record.

Answers

Answer: b

Explanation: I took the test

Cyanobacteria are important to the evolution and advancements of other life forms on Earth because they: B. produced oxygen from photosynthesis.

What are cyanobacteria?

Cyanobacteria is scientifically referred to as Cyanophyta and it can be defined as a group of single-celled, prokaryotic photosynthetic bacteria that live in aquatic habitats (water) and moist soils.

Additionally, this division of microorganisms are capable of manufacturing their own food through a process known as photosynthesis, which typically leads to a release of oxygen into the ecosystem.

In conclusion, Cyanobacteria are very important to the evolution and advancements of other life forms on planet Earth because they help in the production of oxygen through photosynthesis.

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