coli cells are placed in a growth medium containing lactose: Indicate how the following circumstances would affect the expression of the lactose operon (increase/decrease/no change). (a) Addition of high levels of glucose (b) Lac repressor mutations that prevent dissociation of Lac repressor from the operator (c) A mutation that inactivates bgalactosidase (d) mutation that inactivates galactoside permease (e) A mutation that prevents binding of CRP to its binding site near the lac promoter

Answers

Answer 1

(a) Addition of high levels of glucose: decrease (b) Lac repressor mutations that prevent dissociation of Lac repressor from the operator: decrease (c) A mutation that inactivates bgalactosidase: no change (d) mutation that inactivates galactoside permease: increase (e) A mutation that prevents binding of CRP to its binding site near the lac promoter: decrease



(a) Addition of high levels of glucose would decrease the expression of the lactose operon as glucose inhibits the production of cyclic AMP, which is necessary for the activation of the operon.

(b) Lac repressor mutations that prevent dissociation of Lac repressor from the operator would also decrease the expression of the lactose operon as the repressor would remain bound to the operator and prevent transcription.

(c) A mutation that inactivates β-galactosidase would not affect the expression of the lactose operon as it is necessary for lactose breakdown but not for its regulation.

(d) A mutation that inactivates galactoside permease would increase the expression of the lactose operon as it would lead to an accumulation of lactose in the cell, activating the operon.

(e) A mutation that prevents binding of CRP to its binding site near the lac promoter would decrease the expression of the lactose operon as CRP is necessary for the activation of the operon in the absence of glucose.

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Answer 2

(a) Increase: High levels of glucose inhibit the activity of adenylate cyclase, which in turn decreases the intracellular concentration of cAMP, thereby reducing the activity of the catabolite activator protein (CAP).

CAP normally binds to a site near the lac promoter and enhances RNA polymerase binding and transcription initiation. Therefore, when glucose is present, CAP is less active, and transcription from the lac operon is decreased.

(b) No change: If the Lac repressor is mutated and cannot dissociate from the operator, it will remain bound to the operator, inhibiting transcription of the lactose operon.

(c) Decrease: If β-galactosidase is inactivated, it cannot convert lactose into allolactose, the inducer of the lac operon. As a result, the repressor will remain bound to the operator, and transcription of the lac operon will be decreased.

(d) Decrease: If the galactoside permease is inactivated, lactose cannot enter the cell efficiently. As a result, the intracellular concentration of allolactose, the inducer of the lac operon, will be low, and transcription of the lac operon will be decreased.

(e) Decrease: When cAMP-CAP is absent, transcription of the lac operon decreases. In this case, if the mutation prevents binding of CRP to its binding site near the lac promoter, cAMP-CAP will be absent, and transcription of the lac operon will be decreased.

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Related Questions

3. The picture on the right shows the sequence of nitrogen bases on part
of a DNA strand. Which string of letters below represents the nitrogen
bases in the order they would pair up with this part of the DNA strand?
A. C-T-G-C-A
B. G-A-C-G-T
C. A-G-T-A-C
(the picture is T-C-A-T-G)

Answers

Answer: C

Explanation: Thymine pairs with Adenine and Cytosine pairs with Guanine.

Yalll are smart right well I am give you 50 POINT lol you have to do this correctly :3

Answers

Answer:

Top to bottom: 5, 3, 2, 4, 1

Explanation:

I'll take a shot! Here I go!

5 (Sun) = top; we get all our energy from the sun

Then 3 (plants); plants can convert sunlight into food for themselves via photosynthesis

Then 2; we're talking about herbivores that eat plants, right?

Then 4; these are probably~ carnivores

Then 1; when a living thing dies, these guys consume the corpse and return the energy to the ecosystem

Have a great day!

Answer:

Sun

Primary Producers

Primary Consumers

Secondary Consumers

Decomposers

Explanation:

Energy coming from the sun is converted into chemical energy (or glucose) by plants. Primary consumers (herbivores) eat those plants and gain the energy they produced. Secondary consumers (carnivores probably) eat the primary consumers and gain that energy. Then they die and decomposers (fungi) return their energy into the soil.

What is involuntary muscle​

Answers

Answer:

involuntary muscles can be defined as the muscles that functions or contracts without conscious control and are controlled by the autonomic nervous system.

Answer:

involuntary: A muscle movement not under conscious control e.g. the beating of the heart.

All geologic eras are about the same number of years.
True or false

Answers

Answer:

false

Explanation:

(GIVING BRAINLIEST!!)

Due to altitude, you might find snow here during the summer months.

A) By a lake
B) In the forest
C) In the ocean
D) On a mountain

Answers

Answer:

D

Explanation:

Since the temperature is colder at a higher altitide you can use that knowledge to think about how high these places are relative to eachother. You can then take the highest one for your answer

D

Higher up it is colder

1. How many kinds of nitrogen bases does a DNA molecule have?
A. 2
B. 3
C. 4.
D. 1

Answers

Answer:

4.

Explanation:

Adenine, Thymine, Cytosine, and Guanine

(In RNA Thymine is replaced with Uracil)

What are some agents of mutation that can affect the genes of humans? (Choose all that apply)
failure of repair by ligase

codon errors

poor nutrition

ionizing radiation

Answers

Answer: The correct answers are "ionizing radiation", "codon errors" and "failure of repair by ligase"

Explanation:

I took the test

Some agents of mutation that can affect the genes of humans are ionizing radiation and codon errors as well as failure of repair by ligase.

What is genetics?

Genetics has been known as the study of the heredity as well as the variation of the inherited the characters and the genetic properties and the organism's feature and the characteristics. It has been also known as the branch of biology that has to deal with the study of genetic variation, genes, and the heredity in the organisms.

The main function of the DNA has to carry the information from one generation to the another generation this has been the reason due to which DNA has been also known information molecule.

The main function of mRNA has to work as the immedieter between the protein and DNA which has been utilised by ribosome for the process of direct synthesis of protein, and the function of tRNA has to carry the accurate amino acid to the proteins synthesis site which takes place in ribosome.

Therefore, Some agents of mutation that can affect the genes of humans are ionizing radiation and codon errors as well as failure of repair by ligase.

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Why is dissolving salt in water a physical change?

Answers

Dissolving a solid in liquid, such as table salt in water, is a physical change because only the state of the matter has changed. Physical changes can often be reversed. Allowing the water to evaporate will return the salt to a solid state. ... It has not combined with the water to cause a chemical reaction.

Answer:

Allowing the water to evaporate will return the salt to a solid state. ... It has not combined with the water to cause a chemical reaction.

Can some one help???

Answers

Answer:

what is it you need

Explanation:

the rest of the world uses celsius maybe

To become larger in size in a living thing is called

Answers

Answer:

Growing of organisms is the process of being larger in size

Answer:

Growth or u can say growing

where is the gene found?
what's the function of the gene?
what's the structure of the gene?

Answers

Answer:

gene found in the Dna its in the nucleus

What does the term mutation mean in regards to human genetics?

Answers

Answer:

A mutation is a change in a DNA sequence. Mutations can result from DNA copying mistakes made during cell division, exposure to ionizing radiation, exposure to chemicals called mutagens, or infection by viruses.

Explanation:

A mutation is a change in a DNA sequence

Drag the tiles to the boxes to form correct pairs.
Match the parts of fruits.

Answers

The form correct pairs can be matched with the parts of fruits as;

Mesocarp- flesh stuff inside Endocarp- papery part of seed Exocarp-skin of the fruit

What is pericarp and endocarp?

The epicarp, which is the outermost layer, the mesocarp, which is the middle layer, and the endocarp, which is the inner layer enclosing the ovaries or the seeds, are the three separate layers that make up the pericarp in fleshy fruits.

The fruit's exterior surface is made up of the exocarp, the fruit's top layer. The tissue between the exocarp and endocarp is called the mesocarp. The innermost layer, called the endocarp, is situated just outside the seed coat.

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Which organelles must work together to provide food and energy to a plant cell?

Answers

Answer:

Cells are membrane-bound groups of organelles that work together to allow it to function. Some of the major organelles include the nucleus, mitochondria, lysosomes, the endoplasmic reticulum, and the Golgi apparatus. Plant cells also include chloroplasts, which are responsible for photosynthesis.

Explanation:

Hope this helps have a wonderful day! <3

¿Qué receptores se estimulan al oler la rosa? Explica el proceso a través del cual se percibe el aroma de la rosa.

Answers

Answer:

receptores olfativos especializados localizados en el epitelio olfatorio de la mucosa nasal

Explanation:

Las neuronas receptoras del olor se denominan receptores olfatorios. Los receptores olfatorios son células especializadas capaces de percibir miles de sustancias químicas diferentes y decodificar la información en miles de olores distintos. Estos receptores se localizan en la mucosa nasal de las fosas nasales. En el caso del olor a rosa, como así también cualquier otro olor, las sustancias químicas responsables del olor se dirigen al epitelio olfatorio de la mucosa nasal, donde se unen a receptores específicos localizados sobre filamentos sensoriales (cilios) en las membranas celulares de las neuronas especializadas. La unión desencadena una respuesta en la células neuronales, es decir, una descarga eléctrica que se propaga desde las fosas nasales hasta el cerebro, el cual decodifica la información (el olor) en una región conocida como bulbo olfatorio.

To where does carbon dioxide go?

Answers

Some stays in the air domes taken up by plants

All multicellular algae are what?

Answers

Answer:

Algae (singular: alga) are photosynthetic, eukaryotic organisms that do not develop multicellular sex organs. Algae can be unicellular, or they may be large, multicellular organisms.The multicellular algae develop specialized tissues, but they lack the true stems, leaves, or roots of the more complex, higher plants.

Explanation:

What are the two factors that ultimately decide what organisms can live in an area?


1 temperature and oxygen


2food and water


3food and oxygen


4water and oxygen


5water and temperature

Answers

Answer: 2. Food and water

Explanation: Good luck! :D

What is the yellow structure, and what role does it play in a cell??

Answers

Answer:

Storage of fat

Explanation:

I am very sorry if I'm wrong ✿✿✿

List 5 ways that phosphorous is essential to plants & animal life on Earth.

Answers

Answer:

El fósforo (P) es esencial en todas las formas de vida conocidas, dado que constituye un elemento clave en muchos procesos fisiológicos y bioquímicos. Se trata de un componente presente en todas las células de todos los organismos vivos. El fósforo aparece en estructuras complejas de ADN y ARN que, al contener y codificar la información genética, controlan todos los procesos biológicos en las plantas. Además, el fósforo es un componente fundamental del sistema de transporte de energía en todas las células.

El fósforo no aparece aislado en la naturaleza, sino que se encuentra siempre combinado con otros elementos con los que forma los fosfatos que pueden ser muy complejos y presentarse bajo distintas formas en los suelos, el agua, las plantas, los animales y el hombre. Por tanto, se utilizará la palabra “fósforo” de forma genérica en lugar de identificar el fosfato concreto, aunque en la mayoría de los casos, se ofrecerán valores numéricos como P2O5 .

Hasta una época relativamente reciente, el crecimiento de las plantas y de los animales, y por extensión, la productividad de la agricultura, se veían limitados por la falta de fósforo, ya que anualmente solo se disponía de unas pequeñas cantidades de las rocas y de los minerales del suelo gracias a la acción erosiva de los elementos. Cuando los agricultores comenzaron a utilizar fertilizantes en el siglo XIX, los niveles de fósforo disponible para las plantas en muchos suelos eran todavía muy bajos. Por eso, hasta que no se comenzó a aplicar fósforo, la respuesta a otros nutrientes, especialmente al nitrógeno, era muy pequeña, es decir, que el fósforo era el nutriente limitante del crecimiento de las cosechas.

El fósforo desempeña un papel fundamental en la fotosíntesis, proceso por el que las plantas absorben la energía del sol para sintetizar moléculas de carbohidratos, es decir, de azúcares, que son transportados a los órganos de almacenamiento de las plantas. Este proceso es esencial para todas las formas de vida y constituye el primer paso en la cadena para producir alimentos, piensos y fibras.

Las raíces de las plantas absorben el fósforo del agua presente en el suelo y que se denomina solución acuosa del suelo.

Sin embargo, los compuestos de fósforo no son muy solubles y, como consecuencia, la cantidad de fósforo que la planta puede tomar de la solución acuosa del suelo tiende a ser mucho menor de la que necesita, especialmente cuando la planta se encuentra en un periodo de fuerte crecimiento. Por eso, el fósforo de la solución acuosa del suelo debería reponerse con una frecuencia de diez veces al día en esos periodos.

En un día, una cosecha de rápido crecimiento puede absorber el equivalente a cerca de 2,5 kg de P2O5 por hectárea (una hectárea equivale a 10.000 m2 ). De esto se deduce, por tanto, que es necesario que existan reservas adecuadas de fósforo en el suelo y que esas reservas puedan estar disponibles con facilidad. La mayoría de los suelos no abonados contienen una cantidad demasiado pequeña de fósforo, fácilmente aprovechable, para dar respuesta a la gran demanda de las cosechas, en especial durante ciertos periodos del ciclo de crecimiento. De ahí, que se imponga la necesidad de aplicar fertilizantes que contengan fósforo.

La carencia de fósforo afecta no solo al crecimiento de la planta y al desarrollo y rendimiento de la cosecha, sino también a la calidad del fruto y a la formación de las semillas. Asimismo, la carencia de fósforo puede retrasar la maduración de las cosechas, con lo que se retrasa la recolección y se pone en riesgo la calidad del producto.

Explanation:

What soil horizon is the most important for the growth of plants (agriculture) ?

Answers

Accumulative

the accumulative or B horizon is the most fundamental part of soil from many standpoint

Green anoles predominantly thrived on the full of greenery and less competitive island and have developed shorter legs and bigger bodies, while brown, long-legged, small-bodied anoles thrived on the treeless and competitive mainland. Scientists hypothesized that speciation (2 new species) occurred. Explain how that happened using evidence provided and what you know about speciation.

Answers

Answer:

Anolis is involved in the two green and brown species of anole speciation processes. Speciation is the process caused by evolution that results in the development of collective genetic changes during a period of time due to adaption or geological or environmental conditions.

The evolution of these two species is due to the difference of the niche or geological difference likewise here the niches of both green and brown Anoles among species in relative size as well as color and pattern.

the nose produces a sticky material called mucus, which is moistens the air and traps particles true or false

Answers

Answer:

True

Explanation:

It traps the particles and stops them from reaching your lungs and harming them.

how do you determine amino acid sequencing

Answers

Answer:

there are two main methods used to find the amino acid sequences of proteins. Mass spectrometry is the most common method in use today because of its ease of use. Edman degradation using a protein sequenator is the second method, which is most useful if the N-terminus of a protein needs to be characterized.

Explanation:

This is the answer. Hope it helps

PLZ HELP! WILL MARK YOU BRAINLIEST!

Which of the following are associated with the integumentary system?
a. helps support the body
b. encloses internal body structures
c. site of many sensory receptors
d. melanin production

Answers

Answer:

melanin production

Explanation:

it occurs in the hair or skin

D

The integumentary system consists of the skin, hair, nails, glands, and nerves.

How would you define informed consent?
[Type answer here]

Answers

Informed consent is permission granted in the knowledge of the possible consequences, typically that which is given by a patient to a doctor for treatment with full knowledge of the possible risks and benefits.

Chytrids have some unique characteristics that are different from the other groups of fungi. What characteristics make them unique? Check all that apply.

Their spores are produced in sac-shaped structures.
Their spores are produced in club-shaped structures.
Their reproductive cells have flagella.
Their reproductive cells have several nuclei.
They live in wet places.
They live in dry places.

Answers

Answer:

3 & 5 or C & E

Explanation:

❌ Their spores are produced in sac-shaped structures.

❌Their spores are produced in club-shaped structures.

✔ Their reproductive cells have flagella.

❌ Their reproductive cells have several nuclei.

✔ They live in wet places.

❌ They live in dry places.

edge 2021

Answer:

3 & 5 or C & E

Explanation:

✔ Their reproductive cells have flagella

✔ They live in wet places.

Time4Learning

How is passive transport different from active transport?

A) Passive transport is substances moving from HIGH concentration to LOW concentration.
B) Passive transport requires energy input.
C)Passive transport happens regardless of concentration gradient.
D) Passive transport is substances moving from LOW concentration to HIGH concentration.


ASAP

Answers

Answer:

A

Explanation:

Passive transport does not require energy. The substances follow the concentration gradient, meaning they go from high to low. Active transport goes against the concentration gradient. Substances go from low to high, so it requires energy.

which of these presents ab ethical issue associated with genetic testing on humans?

Answers

Answer:

In health care, genetic testing presents several ethical challenges. Legislation is in place or is being developed to limit access to genetic information, so that employers or insurers cannot discriminate against individuals because of their genotypes.

Explanation:

Answer:

The answer choices were:

Explanation:

A. Genetic testing could cause harmful mutations.

 

B. Genetic testing could improve the diagnosis of disease.

 

C. Genetic testing could cause job discrimination by revealing health risks.  

 

D. Genetic testing could cause ecosystem damage by accelerating evolution.

What are Hox genes?
A. genes that can produce large changes in adult animals during the embryological development
B. genes that are most often used to calculate a molecular clock
C. genes that have never gone through the process of crossing over
D. duplicate genes that are always responsible for speciation​

Answers

Answer:

a

Explanation:

I haven't heard of them

Answer:

A.

Explanation:

Hox genes encode and correct positions of body structures making sure they form in the correct places in the body.

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Let T be the linear transformation defined byT(x1,x2,x3,x4,x5)=6x1+7x2+9x3+8x4.Its associated matrix A is an nm matrix,where n=? and m=? 2) john kay's invention of the "flying shuttle" was instrumental during the industrial revolution because ita) decreased the need for mechanization.b) increased the efficiency of steam engines.) allowed for more food products to be grown.d) sped the production of textiles and clothing,