A.) One method for determining the absolute molecular weight of a protein is by using size-exclusion chromatography (SEC).
B.) Equilibrium binding constants can be determined using gel electrophoresis by using a technique called mobility shift assay.
SEC uses a column filled with porous beads to separate proteins depending on their size and shape. Smaller proteins will become caught in the beads as the protein sample goes through the column, taking longer to elute than bigger proteins. A standard curve can be constructed by graphing the elution volumes of protein standards with known molecular weights against their molecular weights.
A labelled ligand, such as a DNA molecule, is combined with a protein of interest and then run on a gel in this approach. If the protein binds to the ligand, the resulting complex will have a different mobility than the free ligand and will migrate over the gel in a different manner.
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(a) One common method used to determine the absolute molecular weight of a protein is through size exclusion chromatography. This technique separates molecules based on their size and shape by passing them through a stationary phase that contains porous beads. The larger the molecule, the less it can penetrate the beads and therefore it elutes out of the column earlier. By comparing the elution volume of the protein to a set of known molecular weight standards, the absolute molecular weight of the protein can be determined.
(b) Equilibrium binding constants can be determined through gel electrophoresis by using a technique called mobility shift assay. In this technique, a DNA or RNA probe is labeled with a fluorescent or radioactive tag and incubated with a protein of interest. The complex formed between the probe and protein will migrate slower on a gel electrophoresis compared to the free probe. By running the gel electrophoresis under different binding conditions and quantifying the ratio of bound probe to free probe, the equilibrium binding constant can be determined. This method is particularly useful for studying protein-DNA or protein-RNA interactions.
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blood plasma for average adults has a volume of 3100mL. if the density of plasma is 1.020 g/mL, what mass of plasma is in the average adult?
Answer:
Noseeeeeee
Explanation:
Eeeeeeeeeee
Analyze the definition of light-year. Why is it in space we use light-year to
measure distance? *
Answer:
Because the distance is too far to count with any other way
Explanation:
how can you tell if an substance is an elements
Answer:
A substance that cannot be broken down into chemically simpler components is considered an element
Hope that helps:)
What are the effects of colonialism on Africa today?
Colonialism made African colonies dependent by introducing a mono- cultural economy for the territories. It also dehumanized African labour force and traders. It forced Africans to work in colonial plantations at very low wages and displaced them from their land
A 250 mL glass of orange juice cantains 22 grams of sugar. How much sugar is in a two liter (2,000 mL) bottle of orange juice?
Answer: 44000 g
Explanation:
For every 250mL of juice, there is 22g of sugar. So for 2000mL,
2000*22g = 44000g of sugar in the bottle of juice
how many mongo seed are equal to 3.50 moles of mongo seeds?
answer:
2.107 x 10^24 mongo seeds
Answer:
50 moles of many mango seeds = 2.107.10²⁴ mango
Further explanation
A mole is a unit of many particles (atoms, molecules, ions) where 1 mole is the number of particles contained in a substance that is the same amount as many atoms in 12 gr C-12
While the number of moles can also be obtained by dividing the mass (in grams) with the molar mass of element or molecule
With Avogadro's number
N = number of gas particles
No = Avogadro number (6.02.10²³)
n = number of moles
n= N/No
3.50 moles of many mango seeds are comparable to
N mango seeds= 3.5 x 6.02.10²³
N mango seeds=2.107 x 10²⁴..
How does the temperature of the air change with altitude?
How many moles are in sample containing 2.71 x 10^24 atoms of iron?
Answer:
4.5 moles
Explanation:
One mole is equal to 6.022 x 10^23 atoms
2.71 x 10^24 atoms * 1 mol/ 6.022 x 10^23 atoms = 4.5 moles
Wendy wants to find out if a substance is an acid or a base. What are two ways that Wendy could
investigate to find out what kind of substance it is?
Without tasting a colorless liquid, an acid or a base can be determined using indicators.
The three most common indicators to test for acids and bases are: Litmus. Methyl orange. Phenolphthalein.
How do you know something is an acid?One practical method for determining how acidic something is is to use the pH scale. A solution is acidic if its pH is less than 7. If the pH of the solution is 7, it is neutral; if the pH is greater than 7, it is basic.To identify acids and bases, two types of litmus paper are available: red litmus paper and blue litmus paper. When exposed to acidic conditions, blue litmus paper turns red, while red litmus paper turns blue when exposed to basic or alkaline conditions.
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Which statement about an object in motion is correct?
Help
I'll give Branliest to the most efficient and first answer.
1. What major change in our thinking about the path of a moving electron had to be made with the advent of quantum theory?
2. Calculate the wavelength of a space shuttle with a mass of 2.05 x 107kg traveling at a rate of 7.85 x 103 m/s.
Answer:
See explanation
Explanation:
1) With the advent of the quantum theory came the idea that the path of a moving electron can not be exactly determined. We can only talk about the probability of finding the electron within a given region in space.
2) Using the De Broglie's relation;
λ = h/mv
Where;
λ = wavelength
h = Plank's constant
m = mass
v = velocity
λ = 6.6 * 10^-34/2.05 x 10^7 * 7.85 x 10^3
λ = 4.1 * 10^-25 m
If you were standing on a beach during the day time, what direction might you feel a
breeze from? Explain why this happens.
Answer:
hi
Explanation:
if your standing in the beach for like in hour and then the temp will start to drop your body heating will start to go down to .
What type of solution is seltzer water? a liquid-liquid b metal-liquid c gas-liquid
Answer:
(I think its mixture not solution)
Explanation:
Multiphase Mixtures
Carbonated water (or carbonated anything) is a multiphase heterogenous mixture, with gaseous carbon dioxide (CO2) bubbling through liquid water (H2O).
Answer: The answer is C. Gas-Liquid
Explanation: I just took the quiz
83.0 g Lithium Cyanide (LiCN)
calculate this to molar mass and moles. Please
Answer:
32.958 g/mol
2.52 moles
Explanation:
Finding the molar mass:
Use the periodic table to determine the masses of each element
6.94+12.011+14.007=32.958 g/mol
Moles:
83 g*1 mol/32.958 g = 2.5183566964 moles
[tex]\\ \sf\longmapsto LiCN[/tex]
[tex]\\ \sf\longmapsto 7u+12u+14u[/tex]
[tex]\\ \sf\longmapsto 21u+12u[/tex]
[tex]\\ \sf\longmapsto 33u[/tex]
[tex]\\ \sf\longmapsto 33g/mol[/tex]
Given mass=83gNo of moles=Given Mass/Molar mass[tex]\\ \sf\longmapsto \dfrac{83}{33}[/tex]
[tex]\\ \sf\longmapsto 2.5mol[/tex]
Which element is less electronegative than silicon (Si)?
Answer:
C
Explanation:
Carbon has an electronegativity of 2.55, followed by Tin at 1.96, Silicon at 1.90 and the least electronegative would be Lead at 1.87.
Answer:
Carbon
Explanation:
Carbon has an electronegativity of 2.55, followed by Tin at 1.96, Silicon at 1.90 and the least electronegative would be Lead at 1.87
how many moles are in 3.0x10^24 molecules of water
Answer:
3.52 x 10^24 molecules x `1mol/6.02 x 10^23 molecules = 5.85 moles of H2O.
Explanation:
1) What type of molecule is water and why? Show its lewis structure, shape, and
explain what type of compound it is in terms of bond type and symmetry.
2) Which part of the water molecules will attract to the negatively shared balloon
and why? Show how the water molecule aligns itself to the negative part of the
balloon.
(PLeASE HeLP)
Answer:
I DO NOT UNDERSTAND THE QUESTION, BUT THANKS FOR THE COINS/TOKENS!
Explanation:
what creates an electric current in a battery?
A. Atoms dissolving
B. Metals generating
C. Electrons jumping
D. Liquids moving
Answer:
There's a separate chemical reaction happening at the positive electrode, where incoming electrons recombine with ions taken out of the electrolyte, so completing the circuit. The electrons and ions flow because of the chemical reactions happening inside the battery—usually two of them going on simultaneously.
Explanation:
option (B) is right
Metals used in batteries undergo a chemical reaction with the electrolyte and free the electrons. The flow of electrons produces the electric current.
What is the electrical battery?An electric battery can be described as a source of electric power containing of one or more electrochemical cells with external connections.
The positive terminal of the battery is the cathode and its negative terminal is the anode. The negative terminal can be denoted as the source of electrons that will flow via an external electric circuit to the positive terminal.
A redox reaction transforms high-energy reactants into lower-energy products, and the free-energy difference provides the external circuit as electrical energy.
Each cell of the battery contains two half-cells connected in series by an electrolyte containing metal cations. One half-cell includes an electrolyte and the negative electrode and the other half-cell includes an electrolyte and the positive electrode. Cations are reduced at the cathode, while metal atoms are oxidized at the anode.
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Explain why coal, oil, and natural gas are fossil fuels.
Answer:
They are fossil fuels because they were formed because of fossilized remains of animals and plants that lived many years ago. They also have a high carbon content.
Why Doesn't The Earth Usually Feel Solar Winds?
Answer:
When the solar winds approach the Earth, the magnetosphere (part of the Earth's natural magnetic defenses) deflects/repels most of the charged particles and shields our planet's surface from the winds. Sometimes, however, the magnetosphere isn't strong enough to block the Earth from these winds, so they do hit Earth and may cause catastrophic consequences, such as ruin power grids, cut off telecommunications (cell service/calls), etc.
Hope this helps you out! Have a nice day! :)
How does gravity help to demonstrate a cause-and-effect relationship?
Answer:
Gravity helps show a cause and effect by demostarting how one object will have an effect on another object.
Explanation:
If you bounce a ball, it will fly back up, but it will fall down again because of gravity. The cause is GRAVITY and the effect is the ball FALLINg. Now, because o fgravity, when the ball bounces up again, it will only bounce 1/2 the hieght, having another cause and effect. As gravity pushes down on objects, the more an object is effected by it, the more presure it will have.
Magnesium is the limiting reactant in this experiment: Calculate the theoretical yield of MgO for each trial. Determine the percent yield of MgO for each trial. Determine the average percent yield of MgO for both trials.
Answer:
how do u want me to do this
Explanation:
What is the Lewis dot diagram for GaF3
Can someone help me
Answer:
View attached image
describe nucleus of atom
Answer:
the nucleus is made up of protons and neutrons
A chemist determined by measurements that 0.050 moles of tin participated in a chemical reaction. Calculate the mass of tin that participated in the chemical reaction.
Be sure your answer has the correct number of significant digits.
Answer:
Explanation:
MM of I2 = 2 (127 g) = 254 g/mol
0.065 mol I2 x 254g I₂/ 1 mol I₂ = 16.5 g I2
5. Predict which element would have a higher electron affinity: silicon or arsenic. Support your prediction with an explanation.
Answer:
Silicone
Explanation:
According to periodic trends, electron affinity increases as you go right and decreases as you go down. Silicone is above arsenic so I would expect it to have a higher electron affinity.
Katrina inserts a key into her car’s ignition. She turns the key, putting her car into ignition mode. In this mode, some energy is used to increase the temperature of the car’s fuel. This energy allows the fuel to combust and the car to move. What’s this energy called?
A.
absorbed energy
B.
activation energy
C.
chemical energy
D.
released energy
E.
solar energy
Answer:
B
Explanation:
Answer:
the correct answer is B
Explanation:
. ILL GIVE BRAINLY
Why does the top layer of the rainforest receive the most sunlight?
Its tall trees rise above all the other layers.
The plants there have very broad, dark green leaves.
It has many trees and vines.
The trees there are thicker than in the other layers.
Answer:
It has many trees and vines
Explanation:
Answer:it’s tall trees rise above all the other layers
Explanation:
The quiz said so
ANSWER ASAP GIVING BRAINLIEST FIVE STARS AND A HEART!!!
Write a paragraph that explains the differences among incandescent, fluorescent, and laser light.
Answer:
An incandescent light bulb works by heating a filament in the bulb. Fluorescent light is a bright light produced by electricity flowing through a tube filled with ionized gas. Fluorescent light bulbs are more energy-efficient than incandescent bulbs.
Explanation:
How many grams of magnesium oxide would be produced if 3.8 grams of magnesium reacted completely with 2.1 grams of oxygen
Answer:
5.3g of MgO would be produced
Explanation:
Based on the chemical reaction:
2Mg(s) + O₂(g) → 2MgO(s)
1 mole of Mg reacts per mole of oxygen.
To solve this question we need to convert the mass of each reactant to moles in order to find limiting reactant using the chemical equation:
Moles Mg -Molar mass: 24.305g/mol-:
3.8g Mg * (1mol / 24.305g) = 0.1563moles Mg
Moles O₂ -Molar mass: 32g/mol-:
2.1g O₂ * (1mol / 32g) = 0.0656 moles O₂
For a complete reaction of 0.0656 moles of O₂ are required:
0.0656 moles O₂ * (2 mol Mg / 1mol O₂) = 0.132 moles Mg
As there are 0.1563 moles of Mg, Mg is the excess reactant and O₂ the limiting reactant.
0.0656 moles O₂ produce:
0.0656 moles O₂ * (2 mol MgO / 1mol O₂) = 0.132 moles MgO
The mass is -Molar mass MgO: 40.3g/mol-:
0.132 moles MgO * (40.3g / mol) =
5.3g of MgO would be produced