Explain why the absorption spectrum of a molecule is independent of the excitation intensity Explain why the emission spectrum of a molecule is independent of the excitation wavelength 3 How do your answers to 1 &2 play out in the working of a fluorescence microscope Lookup DNA, gene, transcription, FISH, & codon on Wikipedia (our reference book for these topics. With FiSH imaging, you can choose to label either an intron or an exon of a gene. What difference does it make? Lookup DAPI& Hoechst on Wikipedia. Is one preferable to the other? 6. 5 Lookup the Molecular Expressions website for basics of the fluorescence microscope (our reference book for this topic, and all of microscopy)

Answers

Answer 1

The absorption spectrum of a molecule is independent of the excitation intensity because the absorption of light by a molecule is a quantized process and is determined solely by the molecule's energy levels.

The emission spectrum of a molecule is independent of the excitation wavelength because the molecule will always emit photons with energies corresponding to the energy difference between its excited and ground states.

The absorption spectrum of a molecule is determined by the energies of the electronic transitions that can take place in the molecule. These energies are fixed and depend only on the molecular structure and the electronic configuration of the molecule.

The intensity of the absorbed light is proportional to the number of molecules that undergo this transition, and not the intensity of the incoming light.

Similarly, the emission spectrum of a molecule is determined by the energy differences between the excited and ground states of the molecule. Once excited, the molecule will emit photons with energies corresponding to these energy differences, regardless of the excitation wavelength used to excite the molecule.

In a fluorescence microscope, a fluorophore (a molecule that can absorb and emit light) is used to label specific molecules in a sample. When excited with light of a certain wavelength, the fluorophore emits light of a different wavelength, which can be detected and used to form an image.

The independence of absorption and emission spectra from excitation intensity and wavelength ensures accurate labeling and detection of the fluorophore.

DNA is the genetic material that contains genes, which are segments of DNA that encode specific proteins through the process of transcription. Fluorescence in situ hybridization (FISH) is a technique used to visualize specific DNA sequences in cells. Labeling either an intron or an exon of a gene can help identify the location and expression level of that gene.

DAPI and Hoechst are both fluorescent dyes that can bind to DNA and be used for DNA visualization in microscopy. DAPI has higher DNA specificity and less background staining, while Hoechst is less toxic and can penetrate cell membranes more easily.

The Molecular Expressions website provides detailed information on the basics of fluorescence microscopy, including the principles of fluorescence, the components of a fluorescence microscope, and various fluorescence techniques used in microscopy.

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Related Questions

Which of the following statements about viruses is true?
A: A virus belongs to the Archaea Domain.
B: A virus is a single-celled organism.
C: A virus has a cell membrane made of proteins.
D: A virus can evolve.

Answers

Answer:

D: A virus can evolve.

Explanation:

Viruses are not organisms so that rules out all other options.

What happens during translation?
a. Messenger RNA is made from a DNA code.
b. The cell uses a messenger RNA code to
make proteins.
c. Transfer RNA is made from a messenger
RNA code.
d. Copies of DNA molecules are made.

Answers

The cell uses mRNA to code new proteins

What would happen to the production of the high energy sugars if water or carbon dioxide were not
available to plants?
ATD

Answers

Answer:

There would be no production of high energy sugars in the plant.

Explanation:

Plants produce their food (autotrophism) in a process unique to them called PHOTOSYNTHESIS. Photosynthesis is the process whereby plants produce energy-storing sugars (glucose) in the presence of light from sun.

This photosynthetic process combines Carbon dioxide (CO2) and water (H2O) as reactants to form sugar (C6H12O6) and oxygen (O2) as products. The equation for photosynthesis is as follows:

6CO2 + 6H2O → C6H12O6 + 6O2

This means that water and carbon dioxide are strictly needed for the process of photosynthesis to occur in plants. Hence, if water or carbon dioxide were not available to plants, there would be no photosynthetic process and ultimately no production of high energy sugars.

please help me with this biology question.............


Why is the use of electron microscopy important in studying cells ??​

Answers

Explanation:

Electronic microscope helps us to see each every part of the cell. So, it is considered important

Answer:

A cell is the smallest unit of life. Most cells are so small that they cannot be viewed with the naked eye. Therefore, scientists must use microscopes to study cells. Electron microscopes provide higher magnification, higher resolution, and more detail than light microscopes.

Explanation:

hope it's help you

7._________________________ lines your digestive tract and blood vessels. It moves food and blood through your body.

smooth muscle
skeletal muscle
cardiac muscle

Answers

Answer:

Smooth Muscle

Explanation:

In the digestive tract it's called the muscularis mucosa.

What does semiconservative replication refer to?
a
Both strands are new in each new DNA molecule.
Both strands are conserved in each new DNA
molecule.
с
One strand is conserved and one strand is new in
each new DNA molecule.
d none of the above

Answers

Answer:

it is a

Explanation:

Both strands are new in each new DNA molecule.

I NEED HELP! Complete the table of the following element. Element: Chlorine Symbol: Cl


number of Protons:

Neutrons:

Electrons:

Atomic Number:

Atomic Mass:

Answers

Answer:

For example, the atomic mass of chlorine (Cl) is 35.45 amu because chlorine is composed of several isotopes, some (the majority) with an atomic mass of 35 amu (17 protons and 18 neutrons) and some with an atomic mass of 37 amu (17 protons and 20 neutrons).

Hope it helped!!!

:

Protons :17
Neutrons:17
Electrons:17
Atomic number:17
Atomic mass:(protons +neutrons) 17+17=34

While exploring a rock formation, Hiroto finds a rock that has footprints pressed into it. A geologist tells Hiroto that the rock is millions of years old. Which of these claims
is correct about Hiroto's find?
It is not a fossil because footprints are not fossils.
It is not a fossil because only whole organisms are fossils.
It is a fossil only if Hiroto finds actual parts of the organism in rocks nearby.
It is a fossil because footprints of organisms from million of years ago are considered to
be fosss

Answers

Answer:

5 is the answer

Explanation:

Answer:

message me on ig

Explanation:

she.hatezay ill help you there

Lack of medical knowledge

Answers

Ah Yes the lack of medical knowledge something I have

Precautions to take during potato osmosis lab

Answers

First, Take a deep enough cavity which can keep a thin layer of tissue at the base of the potato. Second, Make sure the sugar solution which you use, should be sufficiently high osmotic concentration compared to the cell sap of potato cells.

HELPP
The diagram below shows the similarities and differences of plants and animals complete the diagram by filling in the correct term as follows: heterotrophic and autotrophic and multicellular

Answers

Answer:

Both are multicellular (plants have different cells for the leaves and the stem, animals have skin cells, brain cells etc so they are called multicellular).

Plants are autotrophic - they make their own food (glucose) by photosynthesis

Animals are heterotrophic - they eat other organisms, cannot make their own food.

Explanation:

3 questions


1. Water vapor needs ____ to become rain or snow

a. humidity

b. cool air

c. dust

d. all of the above


2. water droplets fall because they are too ?

a. warm

b. cool

c. heavy

d. cold


3. snow is formed when water droplets

a. form ice crystals

b. melt slightly

c. stick together

d. all of the above


These questions are worth 25 points first one to answer CORRECTLY gets marked brainliest :) good luck

Answers

Answer:

the answers are

1) b

2)c

3) a

Water vapor needs cool air, dust particles, and humidity to become rain or snow. Thus, the correct option is D.Water droplets fall because they are too heavy. Thus, the correct option is C.Snow is formed when water droplets form ice crystals. Thus, the correct option is A.

What is Water vapor?

Water vapor may be defined as the gaseous form of water that is usually dispersed across the atmosphere with the help of wind. This state of water is significantly found in the hydrosphere.

This gaseous form of water is formed from the evaporation or boiling of liquid water or from the sublimation of ice.

Water vapor requires to combine with dust particles and cool air along with atmospheric humidity in order to produce rain or snow. Due to the combination of such compounds, water droplets become too heavy.

Snow or ice is the solid appearance of water which is usually formed from the compaction of crystallized structure.

Therefore, each of the above questions is described.

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How many moles are present in 10, 0 grams of sodium hydroxide (NaOH )

Answers

Answer:0.250 mols

Explanation:

That’s the answer I got it wrong using the guy that previously wrote that the answer is 19 mols his totally wrong don’t trust him

We have that Moles that  are present in 10, 0 grams of sodium hydroxide (NaOH ) is

Moles\ NaOH =0.25moles

From the question we are told

How many moles are present in 10, 0 grams of sodium hydroxide (NaOH )

Generally the equation for the  Molar mass of sodium hydroxide is

[tex](NaOH) = (22.990 + 15.9994 + 1.008) \\\\ {(NaOH)} = 40 g/mol[/tex]

Where

Mass_{NaOH}taken = 10 g.

[tex]Moles\ NaOH =\frac{(mass of NaOH taken)}{(molar mass of NaOH)}\\\\Moles\ NaOH = \frac{10}{(40}\\\\Moles\ NaOH =0.25moles[/tex]

Therefore

Moles that  are present in 10, 0 grams of sodium hydroxide (NaOH ) is

Moles\ NaOH =0.25moles

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Which of the following has the highest reproductive potential?
O a. bacteria
O b. cattle
O c. humans
O d. elephants

Answers

Answer:

A. Bacteria

Explanation:

I asked my teacher hahahahahahahaha

Bacteria as the highest reproductive potential. Hence option a is correct.

What is reproductive potential?

Reproductive potential is defined as the proportional ability of a species to reproduce itself under ideal circumstances. The maximum number of children that a particular organism can produce is known as its reproductive potential. Compared to other species, some have substantially higher reproductive capacity.

When people reproduce more frequently, sooner in life, and at higher rates, their reproductive potential rises. The largest influence on reproductive capacity is early pregnancy. Every living thing must be able to reproduce. All living things produce additional organisms that resemble them.

Thus, bacteria as the highest reproductive potential. Hence option a is correct.

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Crocuses can bloom in the winter, while dahlias bloom in the fall. This is due to different responses to daylight length or _________.
A. Sun rays
B. Photoperiodism
C. phototropism
D. geotropism

Answers

Answer:

It's becoz of sun rays.....

How do antibiotics work? Note: you will not be given credit for simply stating “they prevent bacterial growth” or “they kill bacteria”

Answers

Antibiotics stop infections caused by bacteria, they kill it , and/or keep them from copying themselves or reproducing . antibiotic means against life, so any drugs in your body is technically an antibiotic. they attack the wall or coating surrounding the bacteria

Answer:

here's your answer

Explanation:

May this helps you..

Describe the two signals and pathways that are activated when you touch something and experience pain

Answers

The medial thalamus projects to widespread areas of the forebrain, including the somatosensory cortex. Thus there are two major ascending pathways for pain: a direct lateral spinothalamic pathway and an indirect medial spinoreticulothalamic pathway.

Necesito hacer un mapa mental sobre taxonomia y fuandamento filosofico como lo puedo hacer me ayudan plis es urgente

Answers

Answer:

A taxonomy map of philosophy is created using 5 layer version

Explanation:

The map is drawn in a circular form, the parent is usually the center and then the child or the sub branches are then made around the first- parent circle to show the relation between the parent and its child. These branches can have their own sub branches which are then included around the branches the child of the original parent.

15 Which of the following mutations would have the potential to affect future
generations of a species?
A A frame shift mutation in the X chromosome of a cheek cell
B A chromosomal mutation in the Y chromosome of a kidney cell
CA point mutation in the first chromosome of a sperm cell
D A substitution mutation in the third chromosome of a uterus cell

Answers

Answer:

c i think

Explanation:

I don't know just trying to help hope you have a good day

The answer is c a point mutation in the first chromosome of a sperm cell

Please help!!!! Grizzly bears and polar bears are very closely related, so much so that they can reproduce to form hybrid offspring. Use your understanding of natural selection to describe how polar bears became a separate classification from the grizzly.

Answers

Scientists believe that at first these bears scavenged seal carcasses that had washed ashore, and gradually began to hunt the seals by waiting at the water's edge as the seals surfaced to breathe. This is believed to be an important step in the evolution of a new bear species, the polar bear

Over time the new polar bears were used to the cold climate and learnt how to fish, they then separated from the grizzly bears, they developed new creatures which would help them survive in one of the most dangerous climate.

Polar bears become separate classification from the grizzly by the natural selection known as divergent and adaptive evolutionary change.

Polar bears and grizzly bears are considered as closed to one another so they can interbreed. They both diverged from one another due to the harsh climate of the Arctic and the ecology of the region.

The changes that took place are camouflaging and pigment-free fur-coat that helped them in order to adapt to the different climates.The genetic mutation leads to such Adaptive changes when the polar bears migrated northwards.The populations living in different climates undergo natural selection that is divergent natural selection and the adaptive evolutionary changes that result in different species.

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Which of these structures do plants and algae have in common?

A. phloem

B. stems

C. chloroplasts

D. xylem

(pls answer this and have a good day)

Answers

Answer:

b and c

Explanation:

Can someone help with this ?

Answers

Answer:

to convert ADP to ATP

= first option

Explanation:

so it must be the first option

since in order for this conversion the process stated in the first option must occur.

How long will it take a cross country runner to cover a distance of 350 meters at a speed of 17.5 m/min?

Answers

Answer:

20 minutes

Explanation:

Divide distance by speed to get time

D/S = t

350/17.5 = 20

(Just an fyi you posted this in biology)

How is the brain involved with the senses and what is the relationship to the wat a personreacts to objects?

Answers

Answer:

The senses are the components of our nervous system that capture stimuli from the environment for transmission to the brain, and it is there where the information is processed to issue a response. All sensations are electrical signals that travel through neurons which form a network that communicates all organs with the center of the nervous system, and communicate with each other to transmit the electrical impulse through a process known as synapsis.

Explanation:

The brain is an organ that controls the activity of the body, both its conscious and unconscious functions. It is located in the head and corresponds, therefore, to the encephalon of humans and other vertebrates and is subdivided into forebrain, midbrain and hindbrain.  

The brain acts on the rest of the organism by generating muscle activity or by the production and secretion of chemicals called hormones. Thereby the brain controls responses to changes in the environment. Some basic types of response such as reflexes may be mediated by the spinal cord or peripheral ganglia, but sophisticated intentional control of behavior based on complex sensory information requires the integration of information.

The thalamus, which is located in the central part of the brain, processes and coordinates the messages from the senses that it receives from the body. Five senses are considered to exist: sight, touch, smell, taste and hearing. For that, the information perceived from the outside must reach the brain.

The senses are the components of our nervous system that capture stimuli from the environment for transmission to the brain, and it is there where the information is processed to issue a response. Therefore, all sensations perceived from the outside are electrical signals that travel through neurons. For example, the eyes transform light signals into electrical impulses, which travel to the brain where they are transformed into electrical signals.

Neurons form a network that communicates all organs with the center of the nervous system, and communicate with each other to transmit the electrical impulse through a process known as synapsis, which is mediated by molecules called neurotransmitters. Since the electrical impulse cannot jump directly from one neuron to another, these neurotransmitters are needed. When the active neuron produces them, the next neuron in the network detects them and becomes electrically charged. Once this has happened, it produces neurotransmitters again so that the next one becomes electrically activated. This is how the electrical impulse reaches the brain.

Note: your teacher will review your response to this question to ensure you receive proper credit for your answer. The pH scale is a measurement system that indicates the concentration of _______ in solution.
please help ​

Answers

Answer:

hydrogen and hydroxide ions

Explanation:

The pH scale indicates the concentration of hydrogen ions (H+) and hydroxide ions (OH-) in a solution.


What effect does the deer's behavior have on the survival and reproduction of these two
types of cactus?

PLS HELP

Answers

Answer:

Greatly affected.

Explanation:

The deer's behavior has greatly affected the survival and reproduction of these two  types of cactus because one cactus is eaten by deer while the other is saved from it. The cactus with no spines are eaten by deer which destroy the vegetative as well as reproductive parts of cactus so its survival and reproduction does not occur whereas the other cactus have spines on its body is save from deer and increase in the chances of survival and reproduction occur.

What is the name of the site of transcription?

1. Amino acid
2. Nucleus
3. Cytoplasm
4. Mitochondria

Answers

The eukaryotic nucleus therefore provides a distinct compartment within the cell, allowing transcription and splicing to proceed prior to the beginning of translation. Thus, in eukaryotes, while transcription occurs in the nucleus, translation occurs in the cytoplasm.
The Nucleus is where transcription occurs.
B. Nucleus

hope this helps

It is proper to put the _____________ allele before a recessive allele when determining the genotype of the offspring in a Punnett square.

Answers

Answer:

dominant

Explanation:

the dominant allele is the capital letter (for example, T) whereas the recessive allele is the lowercase letter (for example, t). the dominant allele is usually written first.

It is proper to put the dominant allele before a recessive allele when determining the genotype of the offspring in a Punnett square.

What are alleles?

Alleles are the variation present in the gene or DNA sequence. It is the sequence of nucleotides that encodes the production of the product of a gene.

There are two types of alleles, dominant and recessive, the dominant gene is denoted by a capital letter, and it is placed before the recessive allele.

Thus, in a Punnett square, the dominant allele should come before the recessive allele when determining the genotype of the offspring.

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4. What domain name should replace the question mark in this chart?
5 points
Unicellular Protozoa
Uni/multicellular
algao
Multicollular
heterotroph
cell walls chitin
?
Multicoulluar
autotroph
cell walls
of cellulose
Multicellular
heterotroph
Lack cell walls
O a. Archaea
b. Bacteria
O c. Eukarya
O d. Monera

Answers

Answer:

C. Eukarya

Explanation:

differentiate between thallophyta bryophyta and tracheophyta​

Answers

Answer:

The main difference between Thallophyta Bryophyta and Pteridophyta is the organization of each phylum. Thallphyta consists of algae, fungi, lichens, and cyanobacteria. The plant body of Thallophyta is a thallus. ... The plant body of bryophytes is not differentiated into true stem, roots, and leaves.

Explanation:

Other Questions
Those rattlesnakes that are the most _____________ will pass their _____________ on to the next generation. Triton shows similar cratering to the Lunar highlands, which suggests that it too is an old surface. Jupiter puts back into space twice the energy it gets from the Sun. Telescopically, Jupiter is the most colorful and changeable of the planets. The rings of Saturn occupy the region inside Saturn's Roche limit. Name the compound formed when air reacts with magnesium true/fallse, the boundaries between communities are usually sharply defined and easily discerned. The computed upper deviation rate isA. The maximum rate of deviations that the auditor is willing to accept before deciding not to rely on the control.B. The rate of deviations that the auditor expects to occur in the population.C. A point estimate of the population deviation rate.D. The sum of the sample deviation rate and an appropriate allowance for sampling risk. scab"" is a colloquial term used to describe an employee or agent who reports an employers unlawful conduct or a statutory violation to the authorities. true or false You are flying at 0.97 c with respect to Kara. At the exact instant you pass Kara, she fires a very short laser pulse in the same direction you're heading.After 1.0 s has elapsed on Kara's watch, what does Kara say the distance is between you and the laser pulse? Solve: 8(t + 2) - 7 = 2(t - 2) - 11t = __ software products designed to meet the needs of a broad spectrum of users across a wide variety of industries are called K = 3/5 is a solution to the inequality 15k + < 15 the change in owners' equity due to only revenue and expense transactions is explained by the: multiple choice statement of cash flows. statement of financial position. income statement. tax return. Referring to the codes above, create your own function to calculate par value. That is, you have to complete the body of the following function (bond_value: current bond price, c_rate: coupon rate, ttm: time to maturity, and y: yield). par In three to five sentences, summarize how individuals have used their religion to contribute to American society. The table shows the enrollment in a university class so far, broken down by student type. adult education 7graduate2. undergraduate 9Considering this data, how many of the next 12 students to enroll should you expect to beundergraduate students? The best measure of the standard of living in a country is:A) the nominal GDP per capita.B) the real GDP per capita.C) the unemployment rate.D) the growth rate of productivity. please answer i would appreciate it thank you If the sum of 4th and 14th terms of an sequence is 18,then the sum of 8th and 10 th is what is the claim status when the payer is developing the claim In circle S, a sector is shaded. ZTSU measures 40.CT40(47)SWhat fraction of the interior of the circle is shaded?Simplify your answer.4 cmWhich expression represents the area of the shaded sector in square centimeters(4x)(16)(167) process of assigning tasks to workers to reach the desired capacity is called line balancing. group of answer choices false true