In your own words, explain why it is necessary to include only one chain-terminating/ synthesis-terminating nucleotide in each well of the electrophoresis instrument.

Answers

Answer 1

Answer: DNA sequencing methods employ chain termination nucleotides. Furthermore, if two chain-terminating nucleotides are employed in the same well, determining which strand is ended by which dideoxynucleotide will be impossible.

(its my own words, but i did search it to get this answer, hope it helps!)

Answer 2
Chain termination nucleotides are used in DNA sequencing techniques.Furthermore, it will be impossible to tell which strand is terminated by which dideoxynucleotide if two chain-terminating nucleotides are used in the same well.

Why is it necessary to include only one chain terminating synthesis terminating nucleotide?Only a limited subset of molecules will randomly incorporate dideoxynucleotides when the terminating nucleotide is added in small amounts. All permutations of termination can be achieved because different chain-terminating nucleotides are utilized in the four sequencing procedures.

What is a synthesis terminating nucleotide?Synthesis comes to an end when a ddNTP is added to a chain of nucleotides. This is due to the ddNTP molecule's absence of the 3' hydroxyl group needed to bind to the subsequent nucleotide in the chain.

What is chain termination method of DNA sequencing?The "chain termination method," often known as Sanger sequencing, is a technique for figuring out the nucleotide sequence of DNA. The Sanger Sequence is a method that was created in 1977 by two-time Nobel laureate Frederick Sanger and his colleagues.

What is the purpose of terminating the replicated strands?When two replication forks with opposing polarities come together and combine to form two independent, fully formed double-stranded DNA molecules, DNA replication is stopped.

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Related Questions

Why can Marco hear the music, even though he is not in the same room as the speaker?

Answers

Answer:

Sound Travels

Explanation:

Sound waves can travel, and that makes it so that even though Marco is in a different room, he can hear the music. Depending how loud the music is and how thick the walls car, the sound can travel through the walls.

Describe how the suspects used the chemicals found at the crime scene to make the copper coins look silver.

Answers

The chemicals found at the crime scene although not mentioned must be Sodium zincate.

Discussion:

An interesting demonstration to show plating and alloys is the conversion of copper coins to silver look-alike coins.

A 'copper' coin when dipped into a solution of sodium zincate in contact with zinc. The coin is plated with zinc and appears silver in colour.

This is a common chemistry classroom trick.

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True of False: The 8-Step Problem Solving Process was developed as an intended expansion of OODA Loop.

Answers

The 8-Step Problem Solving Process is vital to solving problems in an organized way and it's an extended form of OODA Loop. Therefore, it's logically true.

The 8-Step Problem Solving Process include the following:

Defining the problem.Clarifying the problem.Defining the goals.Identification of the root cause of the problem.Development of an action plan.Executing action plan.Evaluating the results.Continuous improvement.

It should be noted that the 8-Step Problem Solving Process was developed as an intended expansion of the OODA Loop. Therefore, the statement is true.

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Which of the following are needed to calculate the percent yield? Select all that apply. Question 10 options: Theoretical yield Actual yield Excess yield Limiting yield

Answers

Both theoretical and actual yields would be needed to calculate percent yield.

The percent yield of a reaction is defined as the ratio of the actual yield to that of the theoretical yield. It can be mathematically expressed as:

percent yield = actual yield/theoretical yield x 100%

The actual yield is the amount of a specific product produced from a reaction while the theoretical yield is the stoichiometric amount of the same product.

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A sample of a gas in a 4L container at 300K has a pressure of 1 atm. If the
temperature is increased to 600K and the volume is decreased to 2L , the
pressure is likely to be closest to which of the following? *
O 0.5 atm
1 atm
4 atm
2 atm

Answers

The final pressure of the gas in the container is 4 atm

From the question given above, the following data were obtained:

Initial volume (V₁) = 4 L

Initial temperature (T₁) = 300 K

Initial pressure (P₁) = 1 atm

Final temperature (T₂) = 600 K

Final volume (V₂) = 2 L

Final pressure (P₂) =?

Using the combine gas equation, we can obtain the final pressure of the gas as illustrated below:

[tex]\frac{P_{1} V_{1} }{T_{1}} = \frac{P_{2} V_{2}}{T_{2}}\\\\\frac{1 * 4}{300} = \frac{P_{2} * 2}{600} \\\\[/tex]

Cross multiply

300 × 2 × P₂ = 4 × 600

600 × P₂ = 2400

Divide both side by 600

[tex]P_{2} = \frac{2400}{600} \\\\[/tex]

P₂ = 4 atm

Therefore, the final pressure of gas is 4 atm.

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What would happen if we didn’t have the Milky Way

Answers

the earth would become a rouge planet

Explain what is wrong with this electron dot diagram for the element nitrogen

Answers

Hello, is there anyone here a pharmacy technician?

in hydrogen iodide __________________ are the most important intermolecular forces.

Answers

Answer:

dipole-dipole forces

Explanation:

The important intermolecular force in hydrogen iodide HI is dipole- dipole forces. This force is arising due to the partial charge separation and permanent dipole moment.

What is dipole-dipole force?

There are various intermolecular forces which binds two atoms chemically to form a compound. Ionic bonding, covalent bonding, dipole -dipole force, hydrogen bonding, Van der Waals force etc are some of them.

Dipoles are opposite charges separated by a small distance. Dipole -dipole force is generated between the atoms when they have partial charge separation and this creates a permanent dipole moment.

Hydrogen is an electropositive atom and iodine is slightly electronegative. This difference in electronegativities leads to the bonded electrons more attracted towards iodine result in partial charge separation and dipole-dipole force.

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When a substance changes from a solid to a liquid, is a new substance made?

Answers

Answer:

no

Explanation:

it means melting or fusion so yhe answer is no

A substance changes from a solid to a liquid no its is not the formation of new substance. It is the change of state of the matter.

What are the states of matter?

The states of matter can be defined as one of the several configurations in which matter can exist.

There are basically three states of matter.

Solid -  It is defined as hard, distinct in volume and shape. Atoms and molecules are joined together to form solids.Liquid - It is defined as defined volume with the ability to flow and change shape. Atoms and molecules are only weakly bound together in liquids.Gases - It is defined as no clear shape or volume.

Thus, a  substance changes from a solid to a liquid no its is not the formation of new substance. It is the change of state of the matter.

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complete the word equations below to show how the metals are extracted from their oxides: 1) tin oxide + carbon ---> .........? + .......? 2) lead oxide + carbon ----> ......? + ......?

Answers

Answer:

1.) Tin oxide + Carbon → Tin + Carbon monoxide.

2.) Lead oxide + Carbon → Lead + Carbon monoxide.

These equations show that the metals are being extracted by their oxides by heating with carbon. This is because the metals (Tin and Lead) are less reactive than carbon. Remember, extraction methods of metals are determined by their reactivity. Since the elements tin and lead are less reactive than carbon, the extraction process of heating with carbon is needed.  Remember that when a metal heats with carbon, the carbon displaces the metal from the compound and removes the oxygen from the oxide. Leaving you with the metal and carbon monoxide.

in what area of the united states do the fewest people live​

Answers

Answer:

The most populous state in the United States is California, which has 37,253,956 inhabitants. On the other hand, the least populated state is Wyoming with 493,782 inhabitants.

Answer: Alaska. Alaska is by far the least densely populated of the US states and has 1.3 persons per square mile.

Explanation:

Hope it helped .

Question ( please help me )

1 . Which diagram shows pure substance that are elements

2. Which diagram shows pure substance are compounds

3. Which diagram shows a mixture of compounds

4. Which diagram shows a mixture of elements

5. Which diagram shows a mixture of an element and a compound

Answers

Answer:

1. F, H, E

2. A, D, I

3. G

4. E, B, H

5. C

Explanation:

An unknown element sample has 2 isotopes present. The first isotope has a mass of 6.017 amu and is
7.30% abundant. The second has a mass of 7.018 amu and an abundance of 92.7%. Calculate the
average atomic mass of this element

Answers

Answer:

Calculating Atomic Mass

Change each percent abundance into decimal form by dividing by 100. Multiply this value by the atomic mass of that isotope. Add together for each isotope to get the average atomic mass.

Explanation:

have a nice day

Which of the following describes how photochemical smog is bad for the environment?
1. Decreases crop yields
II. Interferes with amount of sunlight reaching plants
III. Mixes with moisture and becomes acidic
OI and 11
Oll and III
O I and III
O I, II, and III

Answers

Answer:

Its l and ll

Explanation:

it doesn't mix with moisture that would be sulfurous smog

Answer: 1 and 3

qwq rawr

The energy released by a nuclear fusion reaction is produced when
1. energy is converted to mass
2. mass is converted to energy
3. heat is converted to temperature
4. temperature is converted to heat

Answers

Answer:

2

Explanation:

energy produced in nuclear fusion or even fission is due to mass defect and it can be calculated by Einstein equation e=mc2

The statement, that describes energy released by a nuclear fusion reaction is produced is "mass is converted to energy."

What is nuclear fusion?

Nuclear fusion is the process by which two light atomic nuclei merge to produce a single heavier one, releasing huge quantities of energy in the process.

A fusion reaction occurs when two light nuclei combine to form a single heavy nucleus. Because the overall mass of the resulting single nucleus is smaller than the mass of the two initial nuclei, the process releases energy.

That is, nuclear energy refers to the energy released as a result of mass loss during the nuclear fusion processes. Both of these processes transform mass loss into energy in accordance with Einstein's mass-energy ratio, E = [tex]mc^{2}[/tex]. As a result of the fusion reaction, mass is destroyed and energy is released.

Hence, the correct answer is 2.

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¿Cuáles son las características que presentan las enzimas? Explica cada una

Answers

Answer:

Acelera las reacciones químicas.

Se requieren en cantidades mínimas.

Son muy específicos en su acción.

Se ven afectados por la temperatura.

Se ven afectados por el pH.

Algunos catalizan reacciones reversibles.

Algunos requieren coenzimas.

Son inhibidos por inhibidores.

Explanation:

The table below gives the atomic mass and relative abundance values for the three isotopes of element M.

Relative abundance (%) Atomic mass (amu)
78.99 23.9850
10.00 24.9858
11.01 25.9826

What is the average atomic mass (in amu) of element M?
2.86
5.36
24.30
24.98

Answers

Answer:

24.30

Explanation:

Multiply the relative abundance by the atomic mass of each isotope, add the results together, and divide by 100. Then round to the correct amount of significant figures.

(78.99 x 23.9850) + (10.00 x 24.9858) + (11.01 x 25.9826)/100

24.30 is the average atomic mass (in amu) of element M.

What is an atomic mass?

Atomic mass is the quantity of matter contained in an atom of an element.

Multiply the relative abundance by the atomic mass of each isotope, add the results together, and divide by 100. Then round to the correct amount of significant figures.

(78.99 x 23.9850) + (10.00 x 24.9858) + (11.01 x 25.9826)

2430.501576 ÷ 100

24.30

Hence, 24.30 is the average atomic mass (in amu) of element M.

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Why do some experiments blow up?

Answers

Answer:

because of chemical reactions

Answer:

gases expand rapidly because their particles move at high speeds in all directions

what is the answer ??z???????​

Answers

Answer:

I think its Female

Explanation:

Correct me if I was wrong :)

Answer: The answer to this question about the given multiple choices about filling the blank with a missing word in order for you to complete the given statement is "Chest broadens, hips broaden and becomes the rounders. These characters are in the females."

Explanation: I chose B) "Females" for the correct answer in order to fill in the blank with a missing word for you to complete the given statement, so here's the completed statement again, "Chest broadens, hips broaden and becomes the rounders. These characters are in the females.".

I hope my answer with the given multiple choice answer and the given explanation is very helpful to your own chemistry question about the given multiple choices about filling the blank with a missing word in order for you to complete the given statement, please mark me as Brainliest and have a great day! :D

Sincerely,

Jason Ta,

The ambitious of Brainly and the role of The TDSB and WHCI Student of the high school.

Methods used to separate heterogeneous mixtures include the following: check all that apply. *

Decanting
Filtration
Sifting

Answers

Answer:

Filtration, Sifting and Decanting

Explanation:

Heterogenous mixtures can be seperated by filtration and sifting (Also known as sieving). Sifting is the process of parting a mixture using a sieve. Sifting is used to separate solid mixtures that contain molecules of varying sizes. Filtration is the process of separating a liquid from an insoluble solid. A common example is using a funnel and a coffee filiter to seperate the liquide from the insoluable solid. Although there are many other methods for filtration. You can also separate mixtures by decantation. Decantation is the process of separating a liquid from a solid/liquid by removing the liquid layer at the top from the layer of solid/liquid below.

I do believe i need help.....

Answers

Answer:

C,D, and A I believe

Explanation:

A bar of copper (Copper = 0.385 J/g.°C) and a bar of aluminum (Aluminum = 0.897 J/g.°C) have the same mass. Which requires more energy to increase the temperature from 20°C to 30 °C?

Answers

Answer:

Aluminum

Explanation:

which of the following properties of an electron in an atom can be expressed with quantum numbers? energy linear momentum angular momentum spin

Answers

The two properties of an electron which can be expressed with quantum numbers are angular momentum and spin.

The properties of an electron in an atom can be represented using four sets of quantum numbers. These four sets of quantum numbers include;

Principal quantum numberOrbital quantum numberMagnetic quantum numberSpin quantum number

Among the options given in the question, the two properties of an electron which can be expressed with quantum numbers are angular momentum and spin.

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Why is productivity greatest near the ocean’s surface

Answers

Answer:

Sunlight penetrates the top layers of the ocean, so photosynthesis occurs there.

Explanation:

doy CORONITA a quien me ayude rapido que es un examen porfaaa

Answers

Answer:

thank you for the points

What does it mean when an element turns into an ion?.

Answers

Answer:

When a neutral atom gains one or more electrons, the number of electrons increases while the number of protons in the nucleus remains the same. The result is that the atom becomes an anion—an ion with a net negative charge.

Explanation:

How to find formula for ionic compounds.

Answers

Answer:

I am using an example for this in this table it works for any other compound as well.

What is the pressure in millimeters of mercury inside a container of gas.

Answers

Atmospheric pressure is 754.6 mm Hg and the density of mercury is 13.546 g/mL.

most transition metal ions contain partially occupied ________ subshells.

Answers

they contain d subshells

An alpha particle is equivalent to the nucleus of an atom of which element?(1 point)
A hydrogen
B helium
C nitrogen
D carbon
ill mark brainliest!!!

Answers

Answer: An alpha-particle is identical to the nucleus of a normal (atomic mass four) helium atom i.e. a doubly ionised helium atom. Alpha particles (also termed alpha radiation or alpha rays) was the first nuclear radiation to be discovered, beta particles and gamma rays were identified soon after.

Answer: D. Carbon

Explanation: Because I said so.

Hope this helps.

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