protospacer oligonucleotide DNA complementary non-complementary protospacer plasmid DNA labeled strand partner strand Casg CrRNA-sp2 tracrRNA CrRNA-sp1 circular Iinearized plasmld-sp2 Cas9 crRNA-sP? tracrRNA crRNA-SPI Mg"+ 50 nt 6300 Dp 4950 bp 1350 bp 23 nt non-complementary strand binding primer PAM protospacer 2 target DNA non-comp ementary TTut~TCALCTuCTA TTICTALAauCCct TTCCCCRLT-Cti WiWi WM complementary AATA-ICTTCTATTGLGTTLAACA-TTTTTCCCR-F ACCCCTTAACTAXT-5 M ~AUAACUCAAUUUGUAH AE crRNA-sp2 MIM: Deccaccgug Gna oGurcaecuamucccucucetauan CGAMACGACAAACMUACCNAG IME UCCCLcC uuuVuU tracrRNA comd Jementary strand binding primer W CATA CTCRA T ? Fig: 1. Cas9 is a DNA endonuclease guided by two RNA molecules: (A) Cas9 was programmed with 42-nucleotide crRNA-sp2 (crRNA containing 26 n: spacer 2 sequence) in the presence or bsence of 75-nucleotide tracrRNA: The complex was added to 2ni circular or Xhol-linearized plasmid DNA bearing sequence complementary tC spacer 2 and functional PAM crRNA-sp1, specificity control; M, DNA marker; kbp 14 A 23 1 1 | 1 1

Answers

Answer 1

The PAM is a short DNA sequence that is recognized by the Cas9 complex and is required for the cleavage of the target DNA. The target DNA is the DNA sequence that is being modified or edited using the Cas9 complex.

The given question appears to be a collection of terms related to molecular biology and genetic engineering. Cas9 is a DNA endonuclease that is guided by two RNA molecules, crRNA-sp2 and tracrRNA. These molecules form a complex with Cas9 and recognize a specific DNA sequence, called a protospacer, in the target DNA. The protospacer oligonucleotide is a short DNA sequence that is complementary to the protospacer and is used to introduce specific mutations or modifications in the target DNA.
The plasmid DNA is a circular or linearized DNA molecule that can be used as a vector for cloning or expressing genes. The labeled strand partner strand refers to the two complementary strands of DNA that are labeled for visualization purposes. The non-complementary strand binding primer is a short DNA sequence that is used to bind the non-complementary strand of DNA.
The PAM is a short DNA sequence that is recognized by the Cas9 complex and is required for the cleavage of the target DNA. The target DNA is the DNA sequence that is being modified or edited using the Cas9 complex.
In summary, the terms in the question relate to the process of using the Cas9 complex to edit or modify DNA sequences. The answer to the question requires a more specific context or purpose for which these terms are being used.

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Related Questions

A thermochemical equation indicates the absorption or release of heat in addition to the reactants and products involved in the chemical reaction. The amount of energy available for conversion into heat is represented by a triangle H does this term stand for?

Answers

Answer:

A. Enthalpy of the reaction

Explanation:

A Thermochemical equation is a balanced stoichiometric chemical equation that indicates the absorption or release of heat in addition to the reactants and products involved in the chemical reaction. The amount of energy available for conversion into heat is represented by ΔH, a this term which stands for the enthalpy change, ΔH.

The Enthalpy of Reaction or Heat of Reaction is the amount of energy in the form of heat transferred during a chemical reaction that occurs at a constant pressure. It is useful for calculating the amount of energy per mole either released or produced in a reaction, therefore is unit is kJ/mol.

In a reaction where energy is absorbed by the reactants during the formation of products, ΔH is positive. On the other hand, in a reaction where energy is released by the reactants during the formation of products, ΔH is negative.

The enthalpy change, ΔH of reactions are determined experimentally under standard conditions of 1 atm and 25 °C (298.15K). Enthalpy can be measured experimentally through the use of a calorimeter. A calorimeter is an isolated system which has a constant pressure and in which heat loss to the surroundings is minimal.

A chemist analyzes two samples. One sample contains 14g of element x and 6.0 g of element y. The second sample contains 28 g of element x and samples are the same substance

Answers

Answer:

Explanation:

Here's the complete question:

A chemist analyzes two samples.One sample contains 14g of element x and 6.0g of element y.The second sample contains 28g of element x and 6.0g of element y. Does it appear that the two samples are the same substance?

The two samples that we are given in the question are different. They are not the same thing because we can deduce that their components ratio are different.

Sample One contains 14g of element x and 6.0g of element y. The ratio of element x to y is 14/6 = 7/3 = 7:3.

Sample Two contains 28g of element x and 6.0g of element y. The ratio of element x to y is 28/6 = 14/3 = 14:3.

Therefore, they have different ratio for their components and are therefore not thesame.

What evolution was evident in Darwin's finches?
1. beak type
2. leg muscles
3. wing span
4. eyesight

Answers

4. eyesight

explanation:
i’m not 100% bc you didn’t give any info on darwin

Answer:

sorry I don't know the answer hope you understand

What are periods on the periodic table? How many are there?

Answers

A period is a horizontal row of the periodic table. There are seven periods in the periodic table, with each one beginning at the far left. A new period begins when a new principal energy level begins filling with electrons. Period 1 has only two elements (hydrogen and helium), while periods 2 and 3 have 8 elements.

1. compare a mixture and a compound , how are they like? ​

Answers

Answer:

Compound are substances which can be formed by chemically combining two or more elements. Mixtures are substances that are formed by physically mixing two or more substances.

I hope it's helpful!

Answer:

COMPOUND= A compound is a substance that is formed when two or more elements combine chemically .

MIXTURES= Mixtures consist of two or more substances simply mixed together , but not chemically combined .

The process of photosynthesis converts carbon atoms from carbon dioxide into....

OA. hydrogen
OB.oxygen
OC.sugars
OD.salts

Answers

Answer:

B. Oxygen

Explanation:

Answer:

C.

Explanation:

The process of photosynthesis converts those things into the sugar glucose, which it uses to sustain itself. A biproduct of this process is oxygen, but the carbon is used to make sugar. Hope this helps and brainliest is always appreciated!

Helppp
me plzzz!!!!

Answers

Answer:

Explanation:

The sound waves are bocing off the walls.  

Look carefully at the list of objects and order
them from smallest to largest.
ant
atom
cell
dust particle
ping pong ball
Explain why you have them in this order. Be sure
to use a 5 complete thoughts.
:

Answers

Answer:

Atom, cell, dust particle, ant, ping pong ball

Explanation:

Firstly, atoms make up all matter, including each of the items on that list. The only things smaller than atoms are the parts of the atom itself, protons, neutrons and electrons. Because none of those are on that list, atoms have to be the smallest. Next smallest is the cell. Cells are made up of atoms, but they also in part make up dust. You can see a dust particle with the naked eye, but in order to see a cell you must use a microscope. Thus, cells are smaller than dust particles. Next is an ant. Obviously, an ant is bigger than a speck of dust, and is made up of cells and atoms. Lastly all you have is the ping pong ball, which is much larger than the rest of the items. Hope this helps!

All matter, including each thing on the list, is made up of atoms. The protons, neutrons, and electrons that make up an atom are the only particles smaller than atoms.

Thus,  Atoms must be the tiniest object because none of those are included in that list. The cell is the next smallest. Atoms make up cells, but dust also makes up a portion of them.

A dust particle can be seen with the unaided eye, but a microscope is required to see a cell.

Cells are hence smaller than dust particles. An ant comes next. Of course, an ant is composed of cells and atoms and is larger than a particle of dust.

Thus, All matter, including each thing on the list, is made up of atoms. The protons, neutrons, and electrons that make up an atom are the only particles smaller than atoms.

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How can you prove that air is a matter ?

Answers

Answer:

One way to prove that air is made of matter is to blow up a balloon. Before you add air into the balloon, it is empty and shapeless. When you puff air into it, the balloon expands, so you know it is filled with something—air is taking up space. You'll also notice that a balloon filled with air sinks to the ground.

Explanation:

11. Explain Supergiants
are cool yet luminous___
because
(Circle the
correct answer.)
a. they produce more
energy
b. they are farther from
Earth
c. they are unusually large

Answers

The correct answer is C. They are unusually large. I would appreciate if you would mark this is brainlyest answer. Thank you!

which is the odd man out? diamond,coal, graphite, bronze.answer with an explanation​

Answers

define p-block element?

Answer:

[tex]\boxed {\boxed {\sf D. \ Bronze}}[/tex]

Explanation:

Let's analyze the answer choices.

The first answer choices. diamond, coal, and graphite, all have something in common. They are allotropes of carbon. They are made of the same element, but each has a different arrangement of atoms, which leads to different properties.

Bronze is an alloy of copper, tin, and other metals. It's not an allotrope of carbon or related to the other answer choices. Therefore, it is the odd one out.

explain how movements along faults generate earthquakes


Answers

Explanation:

When a plate moves it shifts the earth, and it can cause an earthquake.

A fault is where two of earth's tectonic plates come together. They can slide against the other, causing earthquakes, they can push against each other causing mountains to form, and they can move apart from each other, causing  valleys or trenches.

Heavier noble gases are able to form compounds with other elements under specific conditions because their valence ____are farther from the _____

Answers

Answer:

Heavier noble gases are able to form compounds with other elements under specific conditions because their valence electrons are farther from the nucleus.

Explanation:

The name of noble or inert gas is due to the lack of reactivity with other elements. This is due to its electronic configuration, because its outermost shell or valence shell is always complete, without the need to share, give or receive electrons forming bonds. That is, its outer layer is so stable that the element tends not to react with others except in very specific cases.

These exceptions generally involve the heavier noble gases, such as xenon or radon, capable of forming compounds with fluorine and oxygen. This is because the heavier noble gases have more electron shells than the lighter ones. This characteristic causes the outermost electrons to experience a "shielding" effect due to the action of the inner electrons, and they can then be ionized more easily, since the attraction they receive from the positive charges of the nucleus is weaker. That makes the ionization energy low enough to form stable compounds with more electronegative elements, such as fluorine and oxygen.

Heavier noble gases are able to form compounds with other elements under specific conditions because their valence electrons are farther from the nucleus.

What are the elements in group two called

Answers

Answer: Alkaline-earth metal, any of the six chemical elements that comprise Group 2 (IIa) of the periodic table. The elements are beryllium (Be), magnesium (Mg), calcium (Ca), strontium (Sr), barium (Ba), and radium (Ra). Hopefully i helped

Explanation:

Answer: Alkaline-earth metal

Explanation:

Please help fast. I will give brainliest.

Answers

Answer:39 min

Explanation:

3. Which of the following is NOT an assumption of the
kinetic-molecular theory?
a. Collisions between gas particles are elastic.
b. All the gas particles in a sample have the samel
velocity
c. A gas particle is not significantly attracted or
repelled by other gas particles.
d. All gases at a given temperature have the same aver-
age kinetic energy.

Answers

the answer to this is option B, all the gas particles have the same velocity

true or false the membrane of rer is continuous with the outer layer of the mithochondrial membrane

Answers

Answer:

false

Explanation:

How many liters are there in 28g of B?

Answers

Hi
The volume is 58.1
The moler mass is 10.8
Molar mass details
10.81B (1*10.81)

Hope this helps

Which situation is does NOT occur during a chemical reaction?
bonds are formed
bonds are broken
mass is conserved
atoms are destroyed

Answers

I’m pretty sure it’s bonds are broken

Write the symbol and charge for each individual ion:
11) Beryllium and and phosphorus
12) Cesium and sulfur
13) lithium and Phosphorus
14) Fluorine and rubidium
15) Francium and oxygen

Answers

look it up on the periodic table i attached below

im too lazy to type that all out im sorry but this is one of the easiest things you'll do in chemistry and if you tried you could actually finish all 5 of these in less than a minute instead of taking like 10 min to type 10 problems out separately and making 4 diff posts

20.What do carbon and silicon have in common?

a.They belong to the same column (family), therefore they have the same number of valence electrons.
b.They belong to the same column (family), therefore they have the same number of total electrons.
c.They belong to the same column (family), therefore they have the same number of energy levels.
d.They belong to the same column (family), therefore they have the same number of protons.

Answers


a.They belong to the same column (family), therefore they have the same number of valence electrons.

Four different people are using sound wave technology. Which describe the form of technology most likely being used by each person?

Answers

Answer:

Its D

Explanation:

Ali is using a specialized headphone that cancels the extra noise in the atmosphere.

Megan is using a transmitter to convert electrical signals into radio waves.

Kristoff is using a microphone to convert sound waves into the electrical signals.

Jenna is using the radio to convert radio waves into sound waves.

Answer:

D. Ali is using specialized headphones, Megan is using a transmitter, Kristoff is using a microphone, and Jenna is using a radio.

Explanation:

1s2, 2s2, 2p6, 3s2, 3p6, 4s2
After this atom gains or loses valence electron/s, what will the oxidation number be?

Answers

Answer:

if it gains then it will be negative to whatever number it has gained and the reverse for when it loses

Explanation:

which of the following indicates the cathode rays has a large charge to mass ratio?

Answers

Answer:

Gggg

Explanation:

The indication of the cathode rays that has a large charge to mass ratio is there were deflected towards a positive electrode. The correct option is b.

What are cathode rays?

Cathode rays are the rays that move through the negatively charged electrode the cathode charge to the positively charged electrode which is the anode charge in a vacuum tube.

These rays travel through a pipe in a straight line with speed when there is voltage is applied. They are also called electron beams. There is a cathode ray tube, CRT, which is a vacuum tube on which cathode rays travel.

So, the correct option will be for the cathode rays would be deflected towards a positive charge anode.

Therefore, the correct option is b, there were deflected towards a positive electrode.

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The question is incomplete. Your most probably complete question is given below:

They were easily deflected

There were deflected towards a positive electrode

They were deflected towards a negative electrode

They caused a fluorescent screen to glow

Question 4 How many grams of LiOH are needed to make 100 ml of a 0.1 M solution?​

Answers

Explanation:

0.24 grams of LiOH are needed to make 100 ml of a 0.1 M solution

Which of the following is true about the electron configuration of the noble gas

A. The highest occupied s and p orbitals are partially filled.

B. the highest occupied s and p orbitals are completely filled.

C. the electrons with the highest energy are in a f sub level

D. the electrons with the highest energy are in a d sublevel

Answers

Answer:

Noble gases have very stable electron configuration and does not need to gain electrons, only when they gain energy. 1) For example, krypton is a chemical element with symbol Kr and atomic number 36, which means it has 36 protons and 36 electrons.

Answer: I think it’s B, The highest occupied s and p sub levels are completely filled.

Explanation:

How cold does it really get on Pluto's moon, Charon? (I know this is not a Chem question per se, but is there no Earth Science, nor Space, nor Astronomy, nor General Science category on Brainly?)

Answers

Answer:

Explanation:

After various studies and experiments conducted on Pluto's moon, Charon it has been determined that it gets to minus 459.67 degrees Fahrenheit which would be equivalent to minus 273.15 degrees Celsius. This is the coldest temperature that scientists have registered for Charon. This temperature is nearing absolute zero and puts it colder than Pluto itself since Pluto's temperature is believed to get to minus 400 degrees Fahrenheit which is equivalent to minus 240 degrees Celcius.

Models can be used to make predictions .
truth or false ?

Answers

Answer:

true

...................

The answer would be true hope this helps!

The equation for the complete combustion of ethane (C2H4) is
C2H4(g) + 302(g) → 2C02(g) + 2H2O(g)
If 2.70 moles C2H4 reacts with 6.30 moles Oz, identify the limiting
reactant.

Answers

-6x -8y = 28
6х + 4y =-28

would this be infinite many solutions or no solutions?

Find the percent composition of chromium(|||) fluoride (CrF3), which is commonly used as a dye in textiles

Answers

Answer:

%Cr = 47.71%

%F = 52.29%

Explanation:

To do this, we need the atomic weights of all the elements in the chromiun fluoride, which are the following:

Cr: 51.996 g/mol

F: 18.998 g/mol

With these atomic weights, let's calculate the molecular mass of the chromium fluoride:

MM CrF₃ = (51.996) + (18.998 * 3) = 108.99 g/mol

Now, to get the percent composition, all we have to do is divide the atomic weight of each element by the molecular mass, and then, multiply by 100%:

% = (AW / MM) * 100

Replacing with each element:

%Cr = (51.996 / 108.99) * 100

%Cr = 47.71%

%F = 100 - 47.71

%F = 52.29%

Hope this helps

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