Answer:
Advantages
- Serial dilution is used in microbiology to estimate the concentration or number of cells/organisms in a sample to obtain an incubated plate with an easily countable number of colonies.
- In biochemistry, serial dilution is used to obtain the desired concentration of reagents and chemicals from a higher concentration.
- In pharmaceutical laboratories, serial dilution is performed to receive the necessary concentration of chemicals and compounds as this method is more effective than individual dilutions.
- In homeopathy, homeopathic dilutions are used where a substance is diluted in distilled water or alcohol. It is believed than dilution increases the potency of the diluted substance by activating its vital energy.
Disadvantages
- An error might occur during the propagation of the sample, and the transfer inaccuracies lead to less accurate and less precise transfer. This results in the highest dilution to have the most inaccuracies and the least accuracy.
- Because serial dilution is performed in a stepwise manner, it requires a more extended period of time which limits the efficiency of the method.
- Serial dilution only allows the reduction of bacteria/cells but not the separation of bacteria/cells like in other techniques like flow cytometry.
- This technique also requires highly trained microbiologists and experts in aseptic techniques.
The primary colors of visible light are:
Answer: red, green and blue
Answer:
Correct / true
Explanation:
A seed contains
A) cotyledons
B) xylem
C) stoma
D) phloem
answer:
a) cotyledons
explanation
The ADG1(ADP glucose pyrophosphorylase 1) is involved in flowering timing in plants. You are interested in developing a DNA probe using partial protein sequence information. Part of the amino acid sequence is Glu-Glu-His-Asp-Val-Leu 1. determine the possible antisense oligo nucleotides possible from this amino acid sequence 2. How many total unique different oligonucleotide would need to be produced to cover all possibilities
Answer:
1) UUC-AUG-AUC-AAG-UAA.
2) 48
Explanation:
1) Determine the possible antisense oligo nucleotides possible from this amino acid sequence
Given that the peptide sequence is = Glu-Glu-His-Asp-Val-Leu 1.
we will use a codon table to show the codons and Anticodons for each of the Amino acids
Amino Acid Codons Anticodons
Glu GAA, GAG UUC, CUC
His CAU, CAC AUG, GUG
Asp GAU, GAC AUC, GUC
Val GUU, GUC, GUA, GUG AAG, GAC, UAC,CAC
Leu UUA, UUG, CUU, CUC, CUA, CUG | UAA, CAA, AAG, GAG,UAG,CAG
From the table above
The possible antisense oligo nucleotide sequence is : UUC-AUG-AUC-AAG-UAA.
2) How many total unique different oligonucleotide would need to be produced to cover all possibilities
A desired peptide sequence requires 16 anticodons
Hence the total unique different oligonucleotide needed to be produced to cover all possibilities = 16 *3 = 48
The ADG1(ADP glucose pyrophosphorylase is involved in flowering timing in plants and a DNA probe is made by using partial protein sequence information. Part of the amino acid sequence is Glu-Glu-His-Asp-Val-Leu.
1. To determine the possible antisense oligonucleotides possible from this amino acid sequence:
Since, for every amino acid, there is a specific codon and anticodons are present so according to the given information, the possible antisense oligonucleotides for Glu-Glu-His-Asp-Val-Leu is UUC-AUG-AUC-AAG-UAA.
2) The total number of unique different oligonucleotides that would need to be produced to cover all possibilities is 48
since the amino acid sequence is Glu-Glu-His-Asp-Val-Leu has 16 anticodons and each anticodon have 3 nucleotides so the possible number of oligonucleotides are :
16×3 = 48.
What are codon and anticodon?A codon is a trinucleotide sequence of RNA that corresponds to a specific amino acid.
An anticodon is a trinucleotide sequence complementary to that of a corresponding codon in a messenger RNA sequence.
Thus, according to codon and anticodon, the possible antisense oligonucleotides for Glu-Glu-His-Asp-Val-Leu is UUC-AUG-AUC-AAG-UAA and a number of unique different oligonucleotides that would need to be produced to cover all possibilities is 48.
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Break question
what pets would you want
(p.s. could you please answer one of my questions)
What is the transfer of
heat from one object to
another through direct
contact?
Answer:
condiction
Explanation:
conduction is the transfer of heat between substances that are in direct contact with each other.
Oxygen is a product of the electron transport chain.
True or False
Answer:
true
hope this helps you ☺️☺️
Electron transport chain can be defined as the series of organic molecules and proteins which are embedded in the inner membrane of mitochondria. These molecules are involved in the shutting of electrons from FADH2 and NADH to the [tex]O_{2}[/tex].
The molecular oxygen is the final electron acceptor in this electron transport chain that leads to the formation of water and ATP as the end products. This chain takes place in the mitochondria in both animal and plant cells.
Hence, the given statement is true.
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The first stage of the cell cycle during which the cell matures and prepares to divide and copies
its DNA; The stage the cell stays in the most?
The cell multiplies and creates a copy of its DNA during interphase. In the mitotic (M) phase, the cell divides into two new cells by dividing its cytoplasm and DNA into two sets.
What is the main role of interphase stage of cell cycle?A cell consumes the bulk of its time in what is known as interphase, where it creates, duplicates its chromosomes, and acquires available to split. The cell then moves interphase, goes direct mitosis, and completes dividing.
The cell multiplies and the nuclear DNA is duplicated during interphase. The mitotic phase immediately follows interphase.
Chromosomes that have been duplicated are separated and distributed across daughter nuclei during the mitotic phase. Usually, the cytoplasm is divided as well, producing two daughter cells.
Therefore, The first stage and the stage the cell stays in most is Interphase.
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PLZ HELP ME ASAP
Read the article above and answer the following questions
1. The cardiovascular system includes:
A. the heart
B. blood
C. blood vessels
D. all of the above
2. Which of the following is NOT carried through the body by blood?
A. valves
B. carbon dioxide
C. nutrients
D. oxygen
3. Which of the following carries blood out of the heart?
A. arteries
B. veins
C. blood vessels
D. tubes
4. Which of the following is NOT a part of blood?
A. red blood cells
B. white blood cells
C. gorta
D. plasma
5. What is the heart largely composed of?
A. plasma
B. platelets
C. muscle
D. both B and C
all of the above cause the cardiovacular system help you move your blood
Answer:
1. all above
2. valves
3. arteries
4. gorta
5. muscle
Explanation:
Pls someone help its a very easy question
Answer:
you got it right
Explanation:
Chromosomes are...
A.DNA material coiled up in a pile
B.DNA material in pairs
C. DNA material in single strands
D. DNA material outside of the nuclear membrane
WILL GIVE BRAINLIEST
The hormone insulin is secreted by the pancreas. Which activity is MOST likely to
trigger an increase in the levels of insulin circulating in the blood?
O A. driving
B. eating
O C. jogging
OD. sleeping
Answer:
Eating
Hope this helps! <3
Please help me i really don’t know
Answer:
I think its d or option 4
Explanation:
Please mark me brainliest
the green colour pigment present in leaves chlorophyll true or false
Answer:
True
Explanation:
Chlorophyll produces a green pigment, which is why leaves look green
Answer:
true the green stuff is chlorophyll
Explanation:
What is an amniotic egg?
Answer: The amniotic egg was an evolutionary invention that allowed the first reptiles to colonize dry land more than 300 million years ago. Fishes and amphibians must lay their eggs in water and therefore cannot live far from water. ... Within the chorion is the amnion, the membrane for which the amniotic egg is named.
Explanation:
In a process called transpiration, plants get rid of excess water through pores in the leaves called stomata. This excess water is then released into the atmosphere as part of the water cycle. Which of the following terms best describes how the released water enters the atmosphere?
Group of answer choices
precipitation
condensation
capillary action
evaporation
Answer:
Evaporation
Explanation:
how does biodiversity help with farming
Answer:
biodiversity is the basis of agriculture and our food systems. agricultural biodiversity also performs ecosystem services such as soil and water conservation, maintenance of soil fertility, conservation of biota and pollination of plants, all of which are essential for food production and for human survival.
good luck :)
hopefully, this helps
have a great day !!
The stage in the mentrual cycle usually lasting from 3to five days when fertilization does not occur.
A.ovulation
B.stimulation
C.menustration
D.reproduction
Explain what would happen if the leaf tissue is kept at high temperature during the process of cell isolation
Answer:
Wilting of leaves occur.
Explanation:
if the leaf tissue is kept at high temperature during the process of cell isolation the leaf will shrink because of loss of all water. Due to high, temperature the stomata will be open and water is removed in order to remove heat from the body. A time comes that all water that is present in the cell will be removed and as a result wilting of leaves occur.
______refers to the ability to cause change or do work.
A.friction
B.energy
C.gravity
D.distance
Answer:
B
Explanation:
Ive studied rhis before
Polymerase Chain Reaction (PCR) technology is used for
a. DNA identification
b. DNA repair
c. DNA amplification
d. DNA cleavage
Answer:
The correct answer is c.
Explanation:
Polymerase Chain Reaction (PCR) technology is an in vitro enzymatic method that allows the amplification of a specific DNA sequence, that is, it is the DNA amplification technique by means of the polymerase chain reaction (PCR) is a technique that consists of the in vitro amplification of a specific DNA fragment. To carry out the amplification experiment, it is necessary to know, at least partially, the sequence of the fragment to be amplified (a gene, a part of a gene, a non-coding region, ...). Basically, it is about replicating the same DNA fragment over and over again and, for this, we must do in vitro what cells do in vivo to replicate their DNA. Generally, PCR begins with the denaturation or separation of the DNA double helix by heating the sample to a temperature between 94 and 96 ° C to break the hydrogen bonds that joined them, in this way each chain remains as a template for the synthesis of a new complementary strand of DNA.
Glucose is a large molecule that is transported across the cell
membrane through a gated channel, a type of transport protein
used during what
diffusion
The type of transport protein used during glucose transportion across the cell membrane is facilitated diffusion.
Glucose is the main energetic substrate of the cell and requires a transporter protein in the cell membrane for its entry.
Proteins found on the surface of the membrane intervene in facilitated diffusion, which, when they make contact with the molecules, bind to them and allow their entry.
This route is used by molecules that are larger in size, with properties other than the membrane (polar), or are charged and do not cross the membrane freely; for example glucose.
The transport of glucose across the cell membrane is carried out by two families of membrane proteins:
Sodium-coupled glucose transporters are expressed mainly in epithelia that are responsible for the absorption and reabsorption of nutrients.Glucose transporter (GLUT) are expressed in all cells of the body and allow glucose to move from one compartment to another.
Therefore, we can conclude that glucose enters cells through facilitating transport systems, glucose transporter are expressed in all tissues of the organism and transport glucose through its concentration gradient, hence the name of facilitated diffusion.
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The climate changes on Earth tend to be self-amplified. For example, the Earth is
currently going through a warming trend. As the Earth's temperature rises, the ice caps
and sea ice melt, reflecting less solar radiation. This results in more absorption of the
Sun's heat, causing the Earth's temperature to rise more, and the ice to melt more. This is
an example of what type of system?
Answer:
negative feedback
Explanation:
This is an example of negative feedback system.
What do you mean by negative feeback?Negative feedback occurs when some function of the output of a system, process, or mechanism is fed back in a manner that tends to reduce the fluctuations in the output, whether caused by changes in the input or by other disturbances.
Negative feedback (or balancing feedback) occurs when some function of the output of a system, process, or mechanism is fed back in a manner that tends to reduce the fluctuations in the output, whether caused by changes in the input or by other disturbances.
A negative feedback is a self-regulatory system in which it feeds back to the input a part of a system's output so as to reverse the direction of change of the output.
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3. Calculate the kinetic energy of a paper clip shot from a slingshot. The paper clip's mass is 0.01 kg, and its velocity is 10 m/s.
a) 0.1 J
b) 0.25 J
c) 0.5 J
d) 1 J
Answer:
C
Explanation:
0.01kg*(10m/s)^2/2=0.5
help me plzzzzzzzzzzz
Answer:
box 4, because it has more atoms in the same amount of space
What is active transport?
Answer:
the movement of ions or molecules across a cell membrane into a region of higher concentration, assisted by enzymes and requiring energy.
Explanation:
plz answer ASAP will get ten points
Answer:
the 2nd and 4th
Explanation:
they contain more than one molecule due to them being different elements
A new trait that was never in the gene-pool before is called a
A) Adaptation
B) Mutation
C) Genetic Variation
D) normal
Answer:
B) Mutation
Explanation:
Mutation is a mistake in the Gene's I doesnt mean it's a bad thing or a good thing. It could be both
What is exine is made up of????
what's wrong in asking these types to Question.. it's based on studies
Answer:
sporopollenin
While the exine is composed of sporopollenin, a complex and highly resistant biopolymer containing fatty acids, phenylpropanoids, phenolics and carotenoids, the intine is largely composed of pectin and cellulose.
Explanation:
Name the enzyme diagrammed below.
Light moves differently through different materials. What are some factors that could change the amount of sunlight absorbed by Earth’s surface and atmosphere?