When we pop a synovial bubble, the bubble is filled with nitrogen gas where does this nitrogen gas come from

Answers

Answer 1

Answer:

Nitrogen gas in joints

Escaping gases: Scientists explain that synovial fluid present in your joints acts as a lubricant. The fluid contains the gases oxygen, nitrogen, and carbon dioxide. When you pop or crack a joint, you stretch the joint capsule. Gas is rapidly released, which forms bubbles.


Related Questions

What are the stages of photosynthesis in order?

Answers

The Calvin cycle reactions are organized into three major stages: carbon fixation, reduction, and molecule regeneration.

Photosynthesis takes place in two steps. The chloroplast absorbs energy from the sun during the first step. During this stage of the process, water is utilized, and oxygen is produced. Carbon dioxide is consumed in the second stage, and glucose is generated. C3, C4, and CAM are the three basic kinds of photosynthesis (crassulacean acid metabolism).

I had to recall some of their pathways and mechanisms in college, but I'll focus on what gives one an advantage over another and which crops, forages, and weeds have specialized C3 and C4 photosynthesis.

Learn more about Photosynthesis

https://brainly.com/question/29764662

#SPJ4

Question 13 (2 points)

What is one way a population may decline?

Low death rate

Low emigration

High emigration

Och immigration

Answers

Answer:

High emigration

Explanation:

Emigration, or the movement of individuals out of a population or geographic area, can reduce the number of individuals in a population and cause population decline. High emigration rates can also result in a decrease in genetic diversity, mating opportunities, and ecosystem functioning, which can have negative impacts on the population and its environment.

How many bones are part of axial skeleton?

Answers

The axial skeleton consists of 80 bones. It includes the bones of the skull, vertebral column, ribs, and sternum.

The skull has 22 bones, the vertebral column has 26 bones, the rib cage has 24 bones (12 on each side), and the sternum has 1 bone, for a total of 80 bones in the axial skeleton.

The skull is made up of 22 bones, including the bones of the cranium and the facial bones. The cranium is made up of eight bones that enclose and protect the brain, while the facial bones provide the structure for the face and protect the sensory organs such as the eyes, nose, and mouth.

The vertebral column, also known as the spine or backbone, is made up of 26 bones that are stacked one on top of the other. The vertebrae are separated by intervertebral discs, which provide cushioning and allow for movement of the spine. The vertebral column protects the spinal cord and also provides support for the head and upper body.

Know more about vertebral column here: https://brainly.com/question/29521878

#SPJ4

What role do valves play in returning blood to the heart?

Answers

By opening when blood flows towards the heart or closing when blood flow backward, valves aid in the return the blood to the heart. The movement of blood towards the heart is significantly aided the deep veins.

What heart valve is the most crucial?

Before leaving the heart and travelling through the remainder of the body, oxygen-rich blood must pass through one more valve, the aortic valve. Blood cannot return via the valve to a left ventricle.

Which are the 4 heart valves and what do they do?

Blood is moved by two of the valve, the mitral and tricuspid valves, from the heart's upper chambers (the atria) to its lower chambers (the ventricles). The pulmonary and aortic valves, the other two valves, channel blood through the ventricles to the lungs or the rest of the body.

To know more about blood visit:

https://brainly.com/question/14781793

#SPJ4

Characteristics used to describe a substance are called

Answers

Answer:physical properties

Explanation:

Which structure is not a part of the sporophyte generation?
A) Flower
B) Anther
C) Egg
D) Leaf

Answers

B) Anther structure is not a part of the sporophyte generation

What are the components of sporophyte generation?

Sporophytes are spore-producing plant generations. Algae, bryophytes, angiosperms, and gymnosperms all generate spores. A spore is a microscopic structure that develops into a new plant following germination. Mitotic spores and meiotic spores are the two forms of spores.

Plants have structures called elasters. They are present in liverworts to aid spore dispersal, but are missing in mosses, where spore dispersal is regulated by the peristome.

Learn more about Sporophytes

https://brainly.com/question/24233327

#SPJ4

the ventricles begin to fill during ventricular diastole.

Answers

The ventricles begin to fill during ventricular diastole. True Diastole begins with the aortic and pulmonary valves closing. Intraventricular pressure lowers, while ventricular volume increases only slightly (isovolumetric relaxation).

During ventricular diastole, venous blood enters the left atrium, and when blood pressure within the left atrium exceeds blood pressure in the left ventricle, the mitral valve opens and passive filling of the ventricle occurs.

Diastole is the portion of the cardiac cycle that includes ventricular relaxation, passive and active blood filling into the heart, and the period immediately preceding ejection. The interaction of calcium inside cardiac myocytes is critical to ventricular relaxation at the cellular level.

Learn more about diastole.

https://brainly.com/question/15175692

#SPJ4

Full Question: The ventricles begin to fill during ventricular diastole. True /false

A cell with a large volume will have a more difficult time doing what?

Answers

A cell with a large volume will have a more difficult time efficiently transporting materials throughout its interior.

The transport of molecules such as nutrients, waste products, and signaling molecules is crucial for the proper functioning of a cell. In a cell with a large volume, diffusion of these molecules from one side of the cell to the other can take longer, and this can limit the rate of cellular processes. Additionally, the ratio of surface area to volume in a cell decreases as the cell increases in size, which can make it more difficult for the cell to efficiently exchange materials with its environment.

One way that cells cope with this challenge is by increasing their surface area through the development of specialized structures such as microvilli, which are finger-like projections on the surface of some cells. Another way is by dividing into smaller daughter cells, as occurs during cell division. However, there is a limit to how large a cell can grow and still function efficiently, and this limit varies depending on the specific cell type and environmental conditions.

Learn more about cell  here:

https://brainly.com/question/3142913

#SPJ4

The chemical digestion of proteins begins in the:
a. stomach
b. small intestine
c. mouth
d. pancreas

Answers

The chemical digestion of proteins begins in the stomach. (option a) Stomach acid and the enzyme pepsin break down proteins into smaller peptides to digest.

This can then be further broken down into individual amino acids by other digestive enzymes in the small intestine.

The process of protein digestion begins in the stomach, where proteins are exposed to gastric juice, which contains hydrochloric acid and the enzyme pepsin. Pepsin acts on the proteins, breaking them down into smaller peptides. The acidic environment of the stomach also helps to denature the proteins

Learn more about Digestion:

https://brainly.com/question/882651

#SPJ4

How many lymphatic trunks are normally found in the body?A. 11B. 12C. 13D, 14

Answers

The right lymphatic artery and the bronchus are the ducts in the body. Jugular lymph trunks, descending aorta lymph trunks, Broncho mediastinal capillary trunks, and lumbar lymph trunks are the four pairs of lymph trunks.

In the human body, however many lymphatic tubes are typically present?

There are six total. Right lymphatic conduit and thoracic duct are formed when the lymphatic trunks intersect. What is larger and longer on the left (collection duct). It starts at the level of second lumbar vertebra, just anterior to the spinal column below the diaphragm.

What do the body's lymphatic trunks look like?

Collecting vessels come together to form lymphatic trunks. The nine trunks, which drain lymph from the specified locations, Each of which is found as a single gastrointestinal trunk and in twins left and right, respectively, with each aspect of the body.

To know more about lymphatic visit :

https://brainly.com/question/12857392

#SPJ4

During oxidative phosphorylation, which answer choice best describes how electrons move?

Answers

Therefore, response option (A), which describes the mechanism by which electrons flow down an electron transport chain, resulting in the synthesis of ATP from ADP.

During oxidative phosphorylation, how do electrons move?

Protons are pushed out of the matrix and into the intermembrane space as a result of the electrons moving through the electron transport chain. The electrons eventually reach oxygen, which reacts with protons to create water.

Where are the electron transport proteins for oxidative phosphorylation found in the cell?

The electron transport chain, commonly referred to as the oxidative phosphorylation process, is made up of four protein complexes that work together to combine redox events to create an electrochemical gradient that leads to the synthesis of ATP.

To know more about ATP visit:-

https://brainly.com/question/14637256

#SPJ1

a ________________ trait is expressed when two different alleles for the same trait are present.

Answers

A dominant trait is expressed when two different alleles for the same trait are present.

What is a dominant trait?

Dominаnt, аs relаted to genetics, refers to the relаtionship between аn observed trаit аnd the two inherited versions of а gene relаted to thаt trаit. Individuаls inherit two versions of eаch gene, known аs аlleles, from eаch pаrent.

In the cаse of а dominаnt trаit, only one copy of the dominаnt аllele is required to express the trаit. The effect of the other аllele (the recessive аllele) is mаsked by the dominаnt аllele. Typicаlly, аn individuаl who cаrries two copies of а dominаnt аllele exhibits the sаme trаit аs those who cаrry only one copy. This contrаsts to а recessive trаit, which requires thаt both аlleles be present to express the trаit.

For more information about dominant trait refers to the link: https://brainly.com/question/2808776

#SPJ4

Fill in the blank: The ribosome ______BLANK subunit has _______BLANK binding sites for tRNA molecules.
Question 1 choices
Choice A., large; two
Choice B., small; two
Choice C., large; three , [Your answer, correct]
Choice D., small; three
Choice E., large; four

Answers

The ribosome large sub unit has three binding sites for tRNA molecules.

The correct answer is option C.

A ribosome binding  point, or ribosomal list  point( RBS), is a sequence of nucleotides upstream of the  launch codon of an mRNA paraphrase that's responsible for the reclamation of a ribosome during the  inauguration of  restatement. substantially, RBS refers to bacterial sequences, although internal ribosome entry  spots( IRES) have been described in mRNAs of eukaryotic cells or contagions that infect eukaryotes. Ribosome reclamation in eukaryotes is generally  intermediated by the 5' cap present on eukaryotic mRNAs.

The RBS in prokaryotes is a region upstream of the  launch codon. This region of the mRNA has the  agreement 5'- AGGAGG- 3', also called the Shine- Dalgarno( SD) sequence. The  reciprocal sequence( CCUCCU), called theanti-Shine-Dalgarno( ASD) is contained in the 3 ’ end of the 16S region of the  lower( 30S) ribosomal subunit. Upon encountering the Shine- Dalgarno sequence, the ASD of the ribosome base  dyads with it, after which  restatement is initiated.

Learn more about ribosome binding at

https://brainly.com/question/13071576

#SPJ4

What is the main function of the circulatory cardiovascular system?

Answers

The circulatory system eliminates waste items like carbon dioxide and delivers nutrition, hormones, and oxygen to cells.

Your heart and blood arteries make up your circulatory system, commonly referred to as your cardiovascular system. It functions to carry oxygen and other nutrients to all of your body's organs and tissues. Carbon dioxide and other waste materials are also removed. You need a healthy circulatory system for both your physical and mental well-being. Continue reading to learn more about the circulatory system, how it works, and what you can do to maintain the health of your heart and blood vessels.

To learn more about circulatory follow the link : https://brainly.com/question/29259710

#SPJ4

how can a pedigree be a useful tool for geneticists?

Answers

A pedigree, which also identifies the members of a family who have specific genetic pathogenic variants, traits, and diseases as well as their current health, depicts the relationships between family members.

How useful is pedigree analysis, and what is it?

Pedigree analysis is the investigation of a specific attribute that is passed down from one generation to the next. It is useful to understand how a given trait is inherited as well as whether or not the trait is truly inherited.

How can a pedigree be used to monitor genetic disorders?

A genetic pedigree records information on the health of several generations. The diagnosis of an inherited disorder, identifying an inheritance pattern, and guiding clinical decisions for testing and treatment can all be aided by this information.

To know more about geneticists visit:-

https://brainly.com/question/926019

#SPJ4

which two of these terms are better applied to eukaryotic flagella than to bacterial flagella?

Answers

The terms "microtubules" and "undulipodia" are better applied to eukaryotic flagella than to bacterial flagella.So option a and b are correct.

Eukaryotic flagella are complex organelles composed of a bundle of microtubules arranged in a characteristic 9+2 pattern, which provides support and rigidity to the structure. In addition, the movement of eukaryotic flagella is generated by the sliding of microtubules against each other, powered by dynein motor proteins. This mechanism is quite different from that of bacterial flagella, which rotate like propellers and are powered by a rotary motor at the base.

The term "undulipodia" refers to the undulating, wave-like motion of eukaryotic flagella, which is generated by the coordinated sliding of microtubules. Bacterial flagella, on the other hand, move in a more linear, propeller-like fashion, without undulations.

Learn more about Eukaryotic flagella at : https://brainly.com/question/30271226

#SPJ4

Complete question:

which two of these terms are better applied to eukaryotic flagella than to bacterial flagella?

a.undulipodia

b.microtubules

c.nucleus

d.cilia

mutations that are harmful to cells occur more frequently than those that benefit cells. T/F

Answers

The given statement ''mutations that are harmful to cells occur more frequently than those that benefit cells'' is true because the genetic code is highly conserved, meaning that any changes to it have the potential to disrupt normal cellular functions.

Most mutations that occur are either neutral (having no effect on the organism) or harmful (causing negative effects on the organism). Beneficial mutations, which confer a selective advantage, are relatively rare. This is because the genetic code is highly conserved and mutations that alter it are more likely to disrupt normal cellular functions than to improve them. However, over long periods of time and under certain circumstances, beneficial mutations can accumulate and lead to evolutionary changes.

For example, mutations can disrupt the normal function of genes, leading to abnormal cellular processes and the development of diseases such as cancer. Mutations can also cause structural changes in proteins, altering their ability to carry out their normal functions. Harmful mutations can occur spontaneously during DNA replication, or they can be caused by environmental factors such as radiation, chemicals, or viruses.

Overall, Mutations that are harmful to cells occur more frequently than those that benefit cells.

To learn more about mutations

https://brainly.com/question/13923224

#SPJ4

what A genetic mutation in mice leads to a dysfunctional leptin receptor in the hypothalamus that does not bind lepti?

Answers

A inheritable mutation in mice that leads to a dysfunctional leptin receptor in the hypothalamus that doesn't bind leptin can affect in several physiological consequences.

The hormone leptin, which is produced by fat cells, tells the brain when the body has enough energy reserves, which aids in controlling food input and energy balance. Leptin resistance results from a nonoperating leptin receptor in the hypothalamus, which is unfit to bind leptin. Due to the brain's incapability to descry leptin situations in the blood directly, there's an increase in the feeling of hunger and an increase in food consumption. As a result, the mouse may come fat because it'll eat further than is necessary and accumulate redundant fat.

To know more about leptin, click here,

brainly.com/question/9437980

#SPJ4

Please can someone help me?

Answers

Answer: Crossing over

Explanation: There's not much point in explaining it, as you can see it explained right there, but there you have it, your answer.

I hope this helped you!

Most of the growth of a plant body is the result of a. cell differentiation. b. morphogenesis. c. cell division. d.cell elongation.

Answers

The cause of the majority of a plant's growth. cell differentiation, letter A. morphogenesis is (B). cell division (C). (D) cell lengthening.

What kind of growth development is that?

Growth is a rise in size, much like how a seedling develops into a full-grown tree. There are other types of growth as well, such as the development of your sister's personality since she began daily meditation.

What does life's progress growth?

Growth is the constant expansion of an individual's size through time. It could also be described as one of a living thing's traits. The term "biological growth" refers to development that occurs throughout time in biology. The growth of an organism may continue throughout its existence or stop when that species reaches sexual maturity.

To know more about growth visit:

https://brainly.com/question/28789953

#SPJ4

the plasma ______ separates the internal contents of a cell from the extracellular environment.

Answers

Answer:

the plasma membrane.......

why muscles are not likely to tear from their bones.

Answers

Heart muscles, smooth muscles, and cardiac muscles are not bone-attached. Keep in mind that these muscles are controlled involuntarily. Hollow organs including blood arteries, the digestive system, the bladder, and the uterus may contract because of smooth muscle.

How exactly do muscles defend bones?

Skeletal muscle protects the skeletal system from external impacts by absorbing and transferring the energy of mechanical stress in time and space. This function of skeletal muscle is one of the least understood.

Skeletal muscles are joined to the bones by connective tissues like ligaments or bone in the muscular system. All muscles have two or more points of attachment.

learn more about Skeletal muscle

https://brainly.com/question/12252128

#SPJ4

Need this for biology homework

Answers

A. All components work together

Answer: A. All components work together

in the selection from life on the mississippi, what is the main impression created by twain’s depiction of the steamboat?

Answers

Twain's portrayal of the steamboat mostly conveys an image of majesty and audacity.

What is the main thesis of Life just on Mississippi by Mark Twain?

Life on the Mississippi, a memoir and a work of fiction, explores issues of cultural and technical change and advancement.The importance of observing and the usefulness of travel as just a learning opportunity are two more prominent topics.

What did Twain associate with the Mississippi River?

The Mississippi River, located south of Cairo, continues to represent freedom and escape for Huck and Jim.However, because it is bringing them further into the slave states, the river now now symbolizes peril.

To know more about steamboat visit:

https://brainly.com/question/11480954

#SPJ4

Which of the choices is not needed for DNA replication? A-None of the answer options is correct. B-nucleotides C-enzymes D-ribosomes E-DNA. F-Ribosomes.

Answers

For DNA replication needs nucleotides, enzymes, ribosomes, DNA, and ribosomes. Thus, the choice is not needed for DNA replication is none of the answer options is correct.

Hence, the correct option is A.

RNА polymerаse is аn enzyme thаt trаnscribes RNА from DNА; it is not essentiаl for DNА replicаtion. This enzyme is eаsy to confuse with primаse, whose primаry function is to synthesize the RNА primers necessаry for replicаtion. DNА polymerаse аdd nucleotides during replicаtion, synthesizing the dаughter strаnd from the pаrentаl templаte. Helicаse is responsible for sepаrаting double-strаnded DNА. Single-strаnd binding proteins аre needed to keep DNА from reаnneаling аfter it hаs been denаtured by helicаse.

For more information about DNA replication refers to the link: https://brainly.com/question/16464230

#SPJ4

kentucky bluegrass forms seedheads only in the spring.
Is this statement true or false?

Answers

It is accurate to say that only in the spring does Kentucky bluegrass produce seedheads.

Which of the following best represents the decreasing size of sand, silt, and clay?

Consequently, the sequence shows a correct arrangement of soil particles from smallest to largest in terms of particle size. Silt, sand, clay, and gravel. Annual bluegrass often has a paler green hue than Kentucky bluegrass.

Heat affects bluegrass growth?

Cool-season grass known as Kentucky bluegrass thrives in the fall, winter, and spring when the weather is mild. The heated summer months inhibit its growth. Although Kentucky bluegrass will tolerate little shade, it loves direct sunlight.

To know more about Kentucky bluegrass visit:-

https://brainly.com/question/30467463

#SPJ1

Why do we think that male Neanderthals mated with non-African ancestral Homo sapiens?Because Neanderthal mtDNA sequences are present in the human gene pool, and Neanderthal nuclear DNA sequences are not.Because neither Neanderthal mtDNA sequences nor nuclear DNA sequences are present in the modern human gene pool.Because both Neanderthal mtDNA sequences and nuclear DNA sequences are present in the modern human gene pool.Because Neanderthal nuclear DNA sequences are present in the human gene pool, and Neanderthal mtDNA sequences are not.

Answers

Option d) Because Neanderthal nuclear DNA sequences are present in the human gene pool, and Neanderthal mtDNA sequences are not.

The researchers hypothesized that there could be a connection between a species' digit ratio and sexual strategy because humans are often monogamous. If they are correct, early modern humans and modern humans were both somewhat less monogamous than Neanderthals, who had ratios that fell between the two groups (0.928).

About 100,000 years ago, as the earliest groups of modern humans left Africa, they encountered and interbred with Neandertals, maybe in the fertile Nile Valley, along the Middle Eastern coast, or in the formerly lush Arabian Peninsula.

Learn more about Neanderthals mated

https://brainly.com/question/29724956

#SPJ4

Full Question: Why do we think that male Neanderthals mated with non-African ancestral Homo sapiens?

Because Neanderthal mtDNA sequences are present in the human gene pool, and Neanderthal nuclear DNA sequences are not.Because neither Neanderthal mtDNA sequences nor nuclear DNA sequences are present in the modern human gene pool.Because both Neanderthal mtDNA sequences and nuclear DNA sequences are present in the modern human gene pool.Because Neanderthal nuclear DNA sequences are present in the human gene pool, and Neanderthal mtDNA sequences are not.

arrange the following types of microorganisms in the order in which they evolved on earth?

Answers

The order of the organisms in which they evolved on the earth is: anaerobes, anoxygenic phototrophs, oxygenic phototrophs, aerobes, multicellular organisms.

Anaerobes refers to the organisms who can survive in the absence of oxygen. In fact, these organisms die in the presence of oxygen. The example of anaerobes is:  Fusobacterium, Prevotella, Bacteroides, Actinomyces, Clostridia, etc.

Oxygenic phototrophs are the organisms who perform photosynthesis by using the sunlight energy and water. These organisms consist of the photosystems to absorb the light energy. The essential pigment for photosynthesis is chlorophyll. The examples of such organisms are: green plants, algae and cyanobacteria.

The given question is incomplete, the complete question is:

Arrange the following types of microorganisms in the order in which they evolved on earth?

multicellular organisms, anaerobes, oxygenic phototrophs, aerobes, anoxygenic phototrophs

To know more about anaerobes, here

brainly.com/question/29450536

#SPJ4

A cell membrane protein receptor that produces a multiple pathway response and regulates/coordinates many cell function _____.

Answers

Answer: iii

Explanation:

__________ is the organ of digestion is the main site of absorption.

Answers

Small intestine is the organ of digestion is the main site of absorption. The walls of small intestine absorb water and the digested nutrients into bloodstream. As peristalsis continues, waste products of the digestive process move into large intestine.

What is the function of small intestine?

Small intestine is a long, narrow tube that is located in abdomen and it receives partially digested food from stomach. The walls of small intestine contains millions of tiny and finger-like projections called villi and microvilli, which increases the surface area available for absorption.

As the partially digested food passes through small intestine, nutrients are absorbed through the walls of small intestine and into bloodstream. and these nutrients are carbohydrates, proteins, fats, vitamins and minerals.

To know more about small intestine, refer

https://brainly.com/question/7747321

#SPJ4

Other Questions
how many grams of oxygen can be produced from the decomposition of 100 g of kclo3 HomeworkQuestion 13, 3.3.31HW Score: 75%, 12 of 16 pointsO Points: 0 of 1The larger number is and the smaller number isThe sum of two numbers is 1. Three times the larger number plus two times the smaller number is 18. Find the numbers.mryn LathamClear allSaCheck answer - 360 = (-9(6x - 8) Chemistry help is needed !! Allins test scores are shown below88,90,35,92,82,90PART A (what is the mean, median,mode,and range of his scores.what is the outlier in the data set? PLease help me you guys! How do GPS trackers send their location information back? Does a signal go back to the same satellites that provide the GPS signals, or is it via another channel? To measure primary productivity in a grassland ecosystem, you should _____.A). exclude consumers; periodically mow, collect, and weigh the plants; and calculate plant biomass production per unit timeB). measure the solar energy influx to the siteC). measure the growth of all of the consumers in the systemD). measure the total biomass of organisms per unit area what is the range for the following set of scores? 6,12,9,17,11,4,14. The range value is ___ works particularly well for Another valid range value is___ commonly used with (a. variables with precisely defined upper and lower bounds. b. measurements of continuous variable) can you take nighttime cold medicine during the day? Using the Punnett square diagrams, which phenotype is controlledby a dominant allele?Two Generations of Pea Plant CrossingsA- Y = yellowB- y = greenC- Y = Yellow I will mark you brainiest!A signal word is a figure of speech.A) FalseB) True what is the tax multiplier formula? What happens when the North Node is in Aries? PLEASE HELP ME WITH THIS!!! if a brand creates a perception of greater value through effective marketing, which of the following will be the most likely result?A) create customer value B) identify customer demands C) make selling unnecessary a pair of shoes had an original price of 80$ the shoes are now on sale for 35% off what is the sale price of the shoes.SHOW WORK How do we create distance between ourselves and an uncomfortable topic?A. By using euphemisms to make the topic more amusingOB. By creating offensive euphemismsOC. By ignoring all euphemismsD. By using euphemisms to rename the problem consider a manufacturing process that is producing hypodermic needles that will be used for blood donations. these needles need to have a diameter of 1.65 mm. needles that are too large hurt the donor and needles that are too small will rupture the red blood cells, making the sample unusable. this means that the manufacturing process needs to be closely monitored to detect any significant changes from the desired diameter of 1.65 mm. during every shift, a random sample is taken of several needles and diameters are measured. if a problem is discovered, the manufacturing is stopped until it is corrected. suppose the most recent random sample of 35 needles have an average diameter of 1.64 mm and a standard deviation of 0.07 mm. also suppose the diameters of needles produced by this manufacturing process have a bell-shaped distribution. how would you prepare 10.0 ml of a 0.25% m/v hcl solution if 1% m/v hcl was available? how much 1% m/v hcl is needed? how much distilled water is used?