You have a circular plasmid containing 9 kb of DNA, and you wish to map its EcoRI and BamHI sites. When you digest the plasmid with EcoRI and run the DNA on a gel, you observe a single band at 9 kb. You get the same result when you digest the DNA with BamHI. When you digest with a mixture of both enzymes, you observe two bands, one 6 kb and the other 3 kb in size. Explain these results. Draw a map of the restriction sites.
Answers
Answer:
A nick is created between G and A in GAATTC when using Eco R1 site while a nick is generated between G and G in GGATCC when Bam H1 is used. The difference in the nicks created is the main reason you observe two bands when you digest the circular plasmid with a mixture of both
Explanation:
Circular plasmid containing : 9 kb of DNA
Eco R1 and Bam H1 are known as restriction sites in DNA ( i.e. special sequences found in a DNA ) hence they can be specifically cleaved by the restriction enzymes enabling the insertion of a gene. that is why you will get a same result when you digest the Plasmid with either Eco R1 or Bam H1
but A nick is created between G and A in GAATTC when using Eco R1 site while a nick is generated between G and G in GGATCC when Bam H1 is used. The difference in the nicks created is the main reason you observe two bands when you digest the circular plasmid with a mixture of both
Related Questions
Which one of the following plate tectonic settings would have mountains without active volcanoes?
a. ocean-continent
b. continent-continent
c. ocean-ocean
Answers
Explanation:
continent----continent
Which hot and dry biome is home to large herds of herbivores that feed on many types of grasses?
Answers
Answer:
grasslands
Explanation:
the children prepared a juice drink for their food festival by dissolving powdered juice in water what kind of mixture did they form? a. colloid b. suspension c. solution d. saturated solution
Answers
Given a DNA sequence of ACG, what would be the corresponding RNA sequence? Once you determine the RNA sequence, what would be the corresponding amino acid for the RNA codon? Would a change in one nucleotide of the DNA alter the corresponding amino acid? Explain.
Answers
Answer:
- RNA sequence: UGC
- Amino acid sequence: Cysteine
- Yes, a change in nucleotide will alter the amino acid.
Explanation:
According to this question, a DNA sequence was given as follows: ACG. The process of transcription will produce a RNA sequence from this DNA sequence using complementary base pairing i.e. A-U, G-C etc. Based on this, the mRNA sequence that will result of the DNA sequence above is UGC.
The resulting mRNA transcript is a codon (three nucleotides) that will be used in the process of translation to yield an amino acid. The mRNA sequence: UGC codes for amino acid Cysteine.
- A change in one nucleotide of the DNA will alter the corresponding amino acid because DNA sequence in a particular reading frame is responsible for the production of amino acid. Hence, a slight change in nucleotide might change the reading frame of the sequence and hence give rise to a different amino acid.
The speed with which force is developed by skeletal muscles is determined by the Group of answer choices myosin ATPase isoform. Ca2 ATPase activity. sarcoplasmic calcium concentration.
Answers
Answer:
myosin ATPase isoform.
Explanation:
Skeletal muscle is also known as voluntary muscle and it can be defined as a type of muscle connected with the skeleton by tendons, so as to form a mechanical system that enables the movement of the limbs and other body parts with respect to another. Skeletal are found only in vertebrates.
Basically, the length and shortening velocity determines the amount of force a muscle would generated. When a muscle is at its ideal (resting) length, it generates the greatest amount of force while it generates the least amount of force when stretched or shortened with respect to the resting length
The speed with which force is developed by skeletal muscles is determined by the myosin ATPase isoform.
You have been studying two Hippogriff traits as a senior graduation project at Hogwarts (because you are magical), beak size (big beaks, B, are dominant to small beaks, b) and feather color (brown feathers, F, are dominant to white feathers, f). You mate two brown, big-beaked Hippogriffs and obtain the following results: 15 of the baby Griffs are brown and big-beaked; 4 of the baby Griffs are white are big-beaked; and 1 of the baby Griffs is white and small-beaked. What are the genotypes of the parent Hippogriffs
Answers
Answer:
BbFf is the genotype of parent Hippogriffs
Explanation:
Given -
Big beaks, B, are dominant to small beaks, b.
Brown feathers, F, are dominant to white feathers, f.
Two brown, big-beaked Hippogriffs are mated to produce 15 brown and big-beaked; 4 white and big-beaked; and 1 white and small-beaked offspring
The genotype of parents is BbFf
Transcription is the process of making
Answers
Based on scale of 100 and represents average performance
Answers
Answer:
Ok what is the question good sir/madam?
Answer:
Explanation:
Yes i agree with the other answer SIr or Ma'am but i don't know if this question!
Which statement is true about a red brick?
A red brick reflects red light and absorbs blue and green light.
O A red brick reflects blue light and absorbs red and green light.
A red brick reflects green light and absorbs blue and red light.
O A red brick reflects blue and green light and absorbs red light.
Answers
Answer:
1. red brick reflects red light and absorbs blue and green light.
The statement which is true about a red brick is: A. A red brick reflects red light and absorbs blue and green light.
An electromagnetic spectrum refers to a range of frequency and wavelength that an electromagnetic wave is distributed (extends).
Generally, an electromagnetic spectrum comprises the following radiations:
Gamma rays.Ultraviolet radiation. X-rays. Radio waves. Infrared radiation.Visible light.A visible light can be defined as the range of electromagnetic radiation or wavelength that the human eye can detect or see.
Also, the colors of the visible light spectrum are:
Red.Orange.Blue.Indigo.Green.For a red brick, all red light would be reflected while the other colors such as blue and green light are absorbed.
Read more: https://brainly.com/question/23419308
they enjoy being alone and quiet for a small-time what are they?
Answers
Answer:
Introverts enjoy being alone because of acetylcholine) this chemical in the brain might induce a kind of happy feeling, for the introvert.
The temperature of the earth's surface during different seasons is also affected by
the passage of the sun's rays through the atmosphere.
True
False
Answers
Answer:
True
Explanation:
The sun's light rays produce heat which is actually the only reason why we are not living in a block of ice.
Winter is when we are farther from the sun thus it is colder than the summer when we absorb more of the sun's rays.
The statement "the temperature of the earth's surface during different seasons is also affected by the passage of the sun's rays through the atmosphere" is definitely true.
What is an Atmosphere?An atmosphere may be characterized as a type of layer that significantly consists of a mixture of numerous gases that surrounds any celestial body or planet like the earth, mars, etc. The atmosphere of the earth is composed of about 78% nitrogen, 21% oxygen, and one percent other gases.
The rays of the sun typically produce a significant amount of heat. Due to this, we are not living in a block of ice and get a source of energy directly from the sun.
During winter, the sun is farther from the surface of the sun, so we receive less amount or indirect light but in summer, we absorb more of the sun's rays. Due to this, the temperature increases and we feel extremely hot outside.
Therefore, the statement "the temperature of the earth's surface during different seasons is also affected by the passage of the sun's rays through the atmosphere" is definitely true.
To learn more about Atmosphere, refer to the link:
https://brainly.com/question/24925283
#SPJ6
The microtubules that form in prophase and control the chromatids are called___
Answers
in drosophila melanogaster, the common fruit fly, curved wings "I" and purple eyes "r" are two linked recessive genes found on chromosomes 2. The dominant wild type alleles for those genes are long wings"L" and red eyes "R"
a)describe the expected ratio of offspring in the f2 generation of these genes were located on different chromosomes
b)the results in the f2 generation of these flies were: 40 long winged, red eyes; 40 curved winged, red eyes; 10 long winged, purple eyes, and 10 curved winged, red eyed. how many map units apart are the genes for curved wings and purple eye color?
Answers
Answer:
the other person is right, he had messed up before saying that 60 x 40 = 100, but 60 + 40 = 100. Then I had told him that his answer was wrong and that he should put the answer as 240 instead since 60 x 40 = 240 and not 100.
Explanation:
The expected ratio of offspring in the f2 generation of these genes were located on different chromosomes is 25%
What are chromosomes?A chromosome is a long DNA molecule with part or all of the genetic material of an organism. In most chromosomes the very long thin DNA fibers are coated with packaging proteins; in eukaryotic cells the most important of these proteins are the histones.
Chromosomes allow DNA to be accurately copied during these cell divisions. So one more time. Chromosomes are found in the nuclei of our cells and allow DNA to be accurately copied during cell division.
Chromosomes are thread-like structures located inside the nucleus of animal and plant cells. Each chromosome is made of protein and a single molecule of deoxyribonucleic acid (DNA).
Learn more about chromosomes:
https://brainly.com/question/21297190
#SPJ2
What evidence do we have that all continents were merged into one super-continent called Pangaea 250 million years ago?
Answers
Glacial deposits, specifically till, of the same age and structure are found on many separate continents that would have been together in the continent of Pangaea. Fossil evidence for Pangaea includes the presence of similar and identical species on continents that are now great distances apart.
A type of tissue called _______ tissue is responsible for communicating between the brain and the rest of the body
Answers
Answer:
Nervous tissue is responsible for communicating between the brain and the rest of the body
Answer:
A nerve/nervous tissue
Explanation:
How is dopamine involved in drug addiction?
1) drugs change dopamine into another neurotransmitter.
2) drugs cause the reabsorption of dopamine.
3) drugs decrease dopamine levels in the brain.
4) drugs increase the level of dopamine in the brain
Answer and I will give you brainiliest
Answers
Answer:
The correct answer is - option D.
Explanation:
When an individual takes drugs such as heroin, nicotine, cocaine and methamphetamine increases or promotes the neurochemical reaction that increases the amount of dopamine which is a primary factor in addiction that is secreted by neurons in the brain's reward center.
Higher dopamine levels lead to pleasure in the mind of an addict and it makes people forget the pain or stress and gives immense pleasure.
Thus, the correct answer is - option D.
Ethylene, a hormone found in plants, is produced to ripen fruits. These fruits ripen and drop to the ground where they release seeds. Which two plant systems are interacting when this occurs?
Answers
Answer:
Response and reproduction system
Explanation:
B. Suspension c. Solution
D. Saturated solution
5. Which is a solvent in a cup of coffee?
A. coffee
B. creamer
c. sugar
D. water
6. Why should you shake the liquid medicine before drinking?
A. To make it effective. C. To mix the suspended particles at the bottom.
B. To make it taste better. D. To make it clear.
7. What should be done in a liquid medicine before drinking it?
A. Drink right away after opening. C. Let it stand for 3 minutes
B. Shake well
D. All of these
8. Which of the following is called a universal solvent?
A. water 8. gasoline C. juice D. diesel
m-Directions: For items 1-5 What method are you going
Answers
Answer:
5.c 6.c 7.b 8.a
Explanation:
I hope it helps u :)
Linnaeus based one of his classification categories on plants. He even named the Kingdom Plantae. Which best describes the organisms in this kingdom?
A. They are autotrophic and have eukaryotic cells
B. They are made of prokaryotic cells
C. They are multicellular and do not have cell walls
D. They are always single celled and produce their own food
Answers
Answer:
b
Explanation:
all plants are make of prokaryotic cells.
Because it's most resistant to deterioration, which type of molecule is most often found in molecular fossils?
A. RNA molecules
B. Protein molecules
C. DNA molecules
D. Lipid molecules
Answers
Answer:
Of the four main groups of organic molecules, Lipids are the most resistant to decay. They are also highly insoluble in water. All cells produce this type of organic compound to be used in their membranes and in energy storage. These type of organic molecules are found in kerogens
Exam Guidelines
Exam Instructions
Question 13 of 20 :
Select the best answer for the question
13. What molecular cloning tool do bacteria naturally produce to defend against viral infections?
O A. DNA ligase
O B. Restriction enzymes
O C. Antibiotics
O D. PCR
Mark for review (Will be highlighted on the review page)
Si
Type here to search
о
Answers
Answer:
O B. Restriction enzymes
Explanation:
Restriction enzymes has the ability to recognize specific DNA sequences and cut them in a specific manner in order to produced by the bacteria as a defense mechanism against foreign DNA containing substance such as viruses etc. This restriction enzyme is also called restriction endonucleases. This is a cloning tool that bacteria produced naturally in order to defend itself from the infection of virus.
HELP ME
MARKING BRANELIST
Answers
Answer:
I think the answers probably b
You are studying brain cells and you discover a protein neurotransmitter that you
think might be useful. You know the amino acid sequence of the neurotransmitter.
Explain:
a) how you would isolate and identify the neurotransmitter gene
b) how you would produce multiple copies of the gene for study
c) how you would produce large quantities of the neurotransmitter to see if it could be
used as a potential medication.
e
Answers
Which resource is nonrenewable? A. Wind B. Trees C. Coal D. Soybeans
Answers
Which term indicates that a single gene has two or more alleles present in a population?
A. Genotype
B. Point mutation
C. Polymorphism
D. Pleiotrophy
Answers
Genetic polymorphism is the existence of multiple alleles of a single gene.
What are genes?Genes are regions of DNA which code for a particular protein or any other gene product.
Genes can exists as a single copy or as multiple copies.
Alleles are alternate forms of a particular gene.
A single gene that has two or more alleles present in a population is known as genetic polymorphism.
Therefore, multiple alleles of a single gene is known as genetic polymorphism.
Learn more about genes at: https://brainly.com/question/1480756
#SPJ2
Which is an adaptation that helps birds maintain a stable body temperature?
air sacs connected to lungs
large chest muscles
down feathers
nearly hollow bones
Answers
down feathers
Answer:
the answer is down feathers. Or C
Explanation:
I just took the unit review test
Sexual harassment in the workplace is a crime when committed by anyone, regardless of position, role, or gender. Please select the best answer from the choices provided ОТ OF
Answers
Answer:
The answer is true.
In a professional environment, sexually degrading comments and actions are legally prohibited.
PLEASE HELP ME!!!!!!!
Answers
Answer:
The last one
Explanation:
Protein synthesis consumes four high-energy phosphate bonds per added amino acid. Transcription consumes two high energy phosphate bonds per added nucleotide. Calculate how many protein molecules will have been made from an individual mRNA at the point when the energy cost of translation is equal to the energy cost of transcription. Assume that the nucleotides in the coding portion of the mRNA constitute 5% of the total that are transcribed.
Answers
Answer:
30 protein molecules per mRNA molecule
Explanation:
In this problem, it is necessary to have into account that the transcriptional process requires six (6) phosphate bonds to synthesize one (1) codon (i.e, each three nucleotides), and also discards 95% more energy to make mRNA, it means 19 times this amount of energy >> 6 x 19 = 114 bonds. In consequence, transcription requires 120 phosphate bonds (6 + 114 = 120), while translation requires four (4) phosphate bonds per codon. From this deduction, it is possible to find the number of protein molecules synthesized with regard to the energy cost of the translation process and the transcriptional process >>> 120 bonds (transcription) /4 bonds (translation) = 30 protein molecules.
can anyone answer that
Answers
Answer:
1. Short-term energy storage
2. Lipids
3. Speed up chemical reactions, support cell's structure
4. Nucleic acids
Explanation:
The image comprising of a tabular representation portrays the four biological molecules, their building blocks and function. The four biological molecules in nature are carbohydrates, proteins, lipids and nucleic acids.
- Carbohydrates are biological polymers made up of monomers called monosaccharides. They function to store energy in cells for a short period of time. Examples are glucose, starch etc.
- Lipids are formed from monomers called FATTY ACIDS. They function in the long term storage of energy. Examples are phospholipids, fats etc.
- Proteins are formed from monomers called AMINO ACIDS. They provide various functions including speeding up of chemical reactions, provide cellular structure support etc. Examples are haemoglobin, melanin, hormones etc.
- Nucleic acids are polymers of NUCLEOTIDES. They function majorly in the storage of genetic information. Examples are DNA, RNA etc.