you have been studying the effect of loss-of-function mutations in the cdk inhibitor protein (cki) p21. you add the drug fucoxanthin to cell cultures harboring either wild-type or mutant versions of the p21 gene. fucoxanthin is known to induce cell-cycle arrest in g1. after a day, you add the thymidine analog brdu to the culture media, collect the cells after an hour, treat them with anti-brdu antibody and the fluorescent dna stain dapi, and finally subject them to fluorescence-activated cell sorting (facs or flow cytometry). the facs data can be viewed as a two-dimensional dot plot composed of thousands of dots, in which each cell is represented by one dot at coordinates that correspond to the intensities of the dapi fluorescence signal (x axis) and brdu fluorescence signal (y axis) for that cell. answer the following question according to the simplified dot plot below, generated from your experiment. which one of the facs plots (a or b) would you expect to correspond to the loss-of-function p21 mutants?

Answers

Answer 1

FACS plot B would be expected to correspond to the loss-of-function p21 mutants.

FACS (Fluorescence-Activated Cell Sorting) is a technique used to analyze and sort cells based on their fluorescent properties. In this experiment, the dot plot generated from FACS data represents the intensities of the DAPI fluorescence signal (x-axis) and the BrdU fluorescence signal (y-axis) for each cell.

Loss-of-function mutations in the CDK inhibitor protein p21 are expected to disrupt the cell cycle arrest induced by fucoxanthin, the drug added to the cell cultures. As a result, the loss-of-function p21 mutants would not effectively respond to the drug's G1 cell cycle arrest induction.

Based on this information, we can analyze the dot plot to determine which plot corresponds to the loss-of-function p21 mutants. If the loss-of-function p21 mutants are unable to arrest in the G1 phase, they would continue to progress through the cell cycle and incorporate the thymidine analog BrdU during DNA synthesis. Thus, we would expect to observe higher BrdU fluorescence signal (y-axis) in the FACS plot corresponding to the loss-of-function p21 mutants.

Therefore, FACS plot B would likely correspond to the loss-of-function p21 mutants, as it would exhibit a higher intensity of the BrdU fluorescence signal (y-axis) compared to FACS plot A, which would represent the wild-type p21 or a non-mutated control group.

Learn more about Fluorescence-Activated Cell Sorting here:

https://brainly.com/question/30971294

#SPJ11


Related Questions

What are Hox genes?
A. genes that can produce large changes in adult animals during the embryological development
B. genes that are most often used to calculate a molecular clock
C. genes that have never gone through the process of crossing over
D. duplicate genes that are always responsible for speciation​

Answers

Answer:

a

Explanation:

I haven't heard of them

Answer:

A.

Explanation:

Hox genes encode and correct positions of body structures making sure they form in the correct places in the body.

Why is dissolving salt in water a physical change?

Answers

Dissolving a solid in liquid, such as table salt in water, is a physical change because only the state of the matter has changed. Physical changes can often be reversed. Allowing the water to evaporate will return the salt to a solid state. ... It has not combined with the water to cause a chemical reaction.

Answer:

Allowing the water to evaporate will return the salt to a solid state. ... It has not combined with the water to cause a chemical reaction.

Green anoles predominantly thrived on the full of greenery and less competitive island and have developed shorter legs and bigger bodies, while brown, long-legged, small-bodied anoles thrived on the treeless and competitive mainland. Scientists hypothesized that speciation (2 new species) occurred. Explain how that happened using evidence provided and what you know about speciation.

Answers

Answer:

Anolis is involved in the two green and brown species of anole speciation processes. Speciation is the process caused by evolution that results in the development of collective genetic changes during a period of time due to adaption or geological or environmental conditions.

The evolution of these two species is due to the difference of the niche or geological difference likewise here the niches of both green and brown Anoles among species in relative size as well as color and pattern.

What is the yellow structure, and what role does it play in a cell??

Answers

Answer:

Storage of fat

Explanation:

I am very sorry if I'm wrong ✿✿✿

Drag the tiles to the boxes to form correct pairs.
Match the parts of fruits.

Answers

The form correct pairs can be matched with the parts of fruits as;

Mesocarp- flesh stuff inside Endocarp- papery part of seed Exocarp-skin of the fruit

What is pericarp and endocarp?

The epicarp, which is the outermost layer, the mesocarp, which is the middle layer, and the endocarp, which is the inner layer enclosing the ovaries or the seeds, are the three separate layers that make up the pericarp in fleshy fruits.

The fruit's exterior surface is made up of the exocarp, the fruit's top layer. The tissue between the exocarp and endocarp is called the mesocarp. The innermost layer, called the endocarp, is situated just outside the seed coat.

Read more about fruits

https://brainly.com/question/25884013

#SPJ1

How would you define informed consent?
[Type answer here]

Answers

Informed consent is permission granted in the knowledge of the possible consequences, typically that which is given by a patient to a doctor for treatment with full knowledge of the possible risks and benefits.

(GIVING BRAINLIEST!!)

Due to altitude, you might find snow here during the summer months.

A) By a lake
B) In the forest
C) In the ocean
D) On a mountain

Answers

Answer:

D

Explanation:

Since the temperature is colder at a higher altitide you can use that knowledge to think about how high these places are relative to eachother. You can then take the highest one for your answer

D

Higher up it is colder

Can some one help???

Answers

Answer:

what is it you need

Explanation:

the rest of the world uses celsius maybe

What are the two factors that ultimately decide what organisms can live in an area?


1 temperature and oxygen


2food and water


3food and oxygen


4water and oxygen


5water and temperature

Answers

Answer: 2. Food and water

Explanation: Good luck! :D

how do you determine amino acid sequencing

Answers

Answer:

there are two main methods used to find the amino acid sequences of proteins. Mass spectrometry is the most common method in use today because of its ease of use. Edman degradation using a protein sequenator is the second method, which is most useful if the N-terminus of a protein needs to be characterized.

Explanation:

This is the answer. Hope it helps

PLZ HELP! WILL MARK YOU BRAINLIEST!

Which of the following are associated with the integumentary system?
a. helps support the body
b. encloses internal body structures
c. site of many sensory receptors
d. melanin production

Answers

Answer:

melanin production

Explanation:

it occurs in the hair or skin

D

The integumentary system consists of the skin, hair, nails, glands, and nerves.

Chytrids have some unique characteristics that are different from the other groups of fungi. What characteristics make them unique? Check all that apply.

Their spores are produced in sac-shaped structures.
Their spores are produced in club-shaped structures.
Their reproductive cells have flagella.
Their reproductive cells have several nuclei.
They live in wet places.
They live in dry places.

Answers

Answer:

3 & 5 or C & E

Explanation:

❌ Their spores are produced in sac-shaped structures.

❌Their spores are produced in club-shaped structures.

✔ Their reproductive cells have flagella.

❌ Their reproductive cells have several nuclei.

✔ They live in wet places.

❌ They live in dry places.

edge 2021

Answer:

3 & 5 or C & E

Explanation:

✔ Their reproductive cells have flagella

✔ They live in wet places.

Time4Learning

All geologic eras are about the same number of years.
True or false

Answers

Answer:

false

Explanation:

To where does carbon dioxide go?

Answers

Some stays in the air domes taken up by plants

PLEASE ANSWER ASAPP!! WILL GIVE BRAINLIEST
Match the following peer pressure tactics to the definitions. (unspoken pressure, rejection, insults, and reasoning)

Communicating verbally and nonverbally

Attempting to convince peers to alter their beliefs

Excluding or ignoring

Dressing a certain way or participating in a certain activity

Answers

Answer:

excluding or ignoring= rejection

Dressing a certain way or participating in a certain activity= unspoken pressure

Attempting to convince peers to alter their beliefs= pressure

Communicating verbally and nonverbally= insults (?)

PLS I RLLY NEED HELP
What are the potential advantages and disadvantages of a major shift from the hard or traditional path of energy development to the soft or visionary path?


(These were the corresponding textbook pages, if needed) Please read the following from the textbook Environmental Science:

7th Edition - Chapter 17

9th Edition - Chapter 14

Answers

It’s terms in the set 10

Answer:

Advantages of following the soft path, the argument here is alternative sources of power such as hydropower, geothermal energy , wind energy , and photovoltaic cells must be developed. This provides a alternative source to remain in a healthy environment and also function as the society we currently live in using the hard path.  Disadvantages of following the hard path result with future generations fearing over the irreversible damage of climate change and the damage done to our atmosphere. The hard path argue that we should continue to operate in the future as we have in the past, except more efficiently. This is close to impossible and will only continue the negative effects the hard path( the path we have been following) results in. The major shift determines the outcome of this world, the futures worries or reliefs and ultimately the survival of humans.

Explanation:

I got 100% on Edge

¿Qué receptores se estimulan al oler la rosa? Explica el proceso a través del cual se percibe el aroma de la rosa.

Answers

Answer:

receptores olfativos especializados localizados en el epitelio olfatorio de la mucosa nasal

Explanation:

Las neuronas receptoras del olor se denominan receptores olfatorios. Los receptores olfatorios son células especializadas capaces de percibir miles de sustancias químicas diferentes y decodificar la información en miles de olores distintos. Estos receptores se localizan en la mucosa nasal de las fosas nasales. En el caso del olor a rosa, como así también cualquier otro olor, las sustancias químicas responsables del olor se dirigen al epitelio olfatorio de la mucosa nasal, donde se unen a receptores específicos localizados sobre filamentos sensoriales (cilios) en las membranas celulares de las neuronas especializadas. La unión desencadena una respuesta en la células neuronales, es decir, una descarga eléctrica que se propaga desde las fosas nasales hasta el cerebro, el cual decodifica la información (el olor) en una región conocida como bulbo olfatorio.

How is passive transport different from active transport?

A) Passive transport is substances moving from HIGH concentration to LOW concentration.
B) Passive transport requires energy input.
C)Passive transport happens regardless of concentration gradient.
D) Passive transport is substances moving from LOW concentration to HIGH concentration.


ASAP

Answers

Answer:

A

Explanation:

Passive transport does not require energy. The substances follow the concentration gradient, meaning they go from high to low. Active transport goes against the concentration gradient. Substances go from low to high, so it requires energy.

List 5 ways that phosphorous is essential to plants & animal life on Earth.

Answers

Answer:

El fósforo (P) es esencial en todas las formas de vida conocidas, dado que constituye un elemento clave en muchos procesos fisiológicos y bioquímicos. Se trata de un componente presente en todas las células de todos los organismos vivos. El fósforo aparece en estructuras complejas de ADN y ARN que, al contener y codificar la información genética, controlan todos los procesos biológicos en las plantas. Además, el fósforo es un componente fundamental del sistema de transporte de energía en todas las células.

El fósforo no aparece aislado en la naturaleza, sino que se encuentra siempre combinado con otros elementos con los que forma los fosfatos que pueden ser muy complejos y presentarse bajo distintas formas en los suelos, el agua, las plantas, los animales y el hombre. Por tanto, se utilizará la palabra “fósforo” de forma genérica en lugar de identificar el fosfato concreto, aunque en la mayoría de los casos, se ofrecerán valores numéricos como P2O5 .

Hasta una época relativamente reciente, el crecimiento de las plantas y de los animales, y por extensión, la productividad de la agricultura, se veían limitados por la falta de fósforo, ya que anualmente solo se disponía de unas pequeñas cantidades de las rocas y de los minerales del suelo gracias a la acción erosiva de los elementos. Cuando los agricultores comenzaron a utilizar fertilizantes en el siglo XIX, los niveles de fósforo disponible para las plantas en muchos suelos eran todavía muy bajos. Por eso, hasta que no se comenzó a aplicar fósforo, la respuesta a otros nutrientes, especialmente al nitrógeno, era muy pequeña, es decir, que el fósforo era el nutriente limitante del crecimiento de las cosechas.

El fósforo desempeña un papel fundamental en la fotosíntesis, proceso por el que las plantas absorben la energía del sol para sintetizar moléculas de carbohidratos, es decir, de azúcares, que son transportados a los órganos de almacenamiento de las plantas. Este proceso es esencial para todas las formas de vida y constituye el primer paso en la cadena para producir alimentos, piensos y fibras.

Las raíces de las plantas absorben el fósforo del agua presente en el suelo y que se denomina solución acuosa del suelo.

Sin embargo, los compuestos de fósforo no son muy solubles y, como consecuencia, la cantidad de fósforo que la planta puede tomar de la solución acuosa del suelo tiende a ser mucho menor de la que necesita, especialmente cuando la planta se encuentra en un periodo de fuerte crecimiento. Por eso, el fósforo de la solución acuosa del suelo debería reponerse con una frecuencia de diez veces al día en esos periodos.

En un día, una cosecha de rápido crecimiento puede absorber el equivalente a cerca de 2,5 kg de P2O5 por hectárea (una hectárea equivale a 10.000 m2 ). De esto se deduce, por tanto, que es necesario que existan reservas adecuadas de fósforo en el suelo y que esas reservas puedan estar disponibles con facilidad. La mayoría de los suelos no abonados contienen una cantidad demasiado pequeña de fósforo, fácilmente aprovechable, para dar respuesta a la gran demanda de las cosechas, en especial durante ciertos periodos del ciclo de crecimiento. De ahí, que se imponga la necesidad de aplicar fertilizantes que contengan fósforo.

La carencia de fósforo afecta no solo al crecimiento de la planta y al desarrollo y rendimiento de la cosecha, sino también a la calidad del fruto y a la formación de las semillas. Asimismo, la carencia de fósforo puede retrasar la maduración de las cosechas, con lo que se retrasa la recolección y se pone en riesgo la calidad del producto.

Explanation:

which of these presents ab ethical issue associated with genetic testing on humans?

Answers

Answer:

In health care, genetic testing presents several ethical challenges. Legislation is in place or is being developed to limit access to genetic information, so that employers or insurers cannot discriminate against individuals because of their genotypes.

Explanation:

Answer:

The answer choices were:

Explanation:

A. Genetic testing could cause harmful mutations.

 

B. Genetic testing could improve the diagnosis of disease.

 

C. Genetic testing could cause job discrimination by revealing health risks.  

 

D. Genetic testing could cause ecosystem damage by accelerating evolution.

1. How many kinds of nitrogen bases does a DNA molecule have?
A. 2
B. 3
C. 4.
D. 1

Answers

Answer:

4.

Explanation:

Adenine, Thymine, Cytosine, and Guanine

(In RNA Thymine is replaced with Uracil)

3. The picture on the right shows the sequence of nitrogen bases on part
of a DNA strand. Which string of letters below represents the nitrogen
bases in the order they would pair up with this part of the DNA strand?
A. C-T-G-C-A
B. G-A-C-G-T
C. A-G-T-A-C
(the picture is T-C-A-T-G)

Answers

Answer: C

Explanation: Thymine pairs with Adenine and Cytosine pairs with Guanine.

To become larger in size in a living thing is called

Answers

Answer:

Growing of organisms is the process of being larger in size

Answer:

Growth or u can say growing

Which organelles must work together to provide food and energy to a plant cell?

Answers

Answer:

Cells are membrane-bound groups of organelles that work together to allow it to function. Some of the major organelles include the nucleus, mitochondria, lysosomes, the endoplasmic reticulum, and the Golgi apparatus. Plant cells also include chloroplasts, which are responsible for photosynthesis.

Explanation:

Hope this helps have a wonderful day! <3

where is the gene found?
what's the function of the gene?
what's the structure of the gene?

Answers

Answer:

gene found in the Dna its in the nucleus

Yalll are smart right well I am give you 50 POINT lol you have to do this correctly :3

Answers

Answer:

Top to bottom: 5, 3, 2, 4, 1

Explanation:

I'll take a shot! Here I go!

5 (Sun) = top; we get all our energy from the sun

Then 3 (plants); plants can convert sunlight into food for themselves via photosynthesis

Then 2; we're talking about herbivores that eat plants, right?

Then 4; these are probably~ carnivores

Then 1; when a living thing dies, these guys consume the corpse and return the energy to the ecosystem

Have a great day!

Answer:

Sun

Primary Producers

Primary Consumers

Secondary Consumers

Decomposers

Explanation:

Energy coming from the sun is converted into chemical energy (or glucose) by plants. Primary consumers (herbivores) eat those plants and gain the energy they produced. Secondary consumers (carnivores probably) eat the primary consumers and gain that energy. Then they die and decomposers (fungi) return their energy into the soil.

the nose produces a sticky material called mucus, which is moistens the air and traps particles true or false

Answers

Answer:

True

Explanation:

It traps the particles and stops them from reaching your lungs and harming them.

Which mealworm behaviors were reflexes

Answers

Being hungry or having a dry mouth is an instinctive behavior in humans. 2 Which mealworm behaviors were reflexes? Why? The ones where the mealworm was touched by feathers or metal pins were reflex behaviors, because they were a stimulus, and the mealworm had no control over its reaction.

What does the term mutation mean in regards to human genetics?

Answers

Answer:

A mutation is a change in a DNA sequence. Mutations can result from DNA copying mistakes made during cell division, exposure to ionizing radiation, exposure to chemicals called mutagens, or infection by viruses.

Explanation:

A mutation is a change in a DNA sequence

What is involuntary muscle​

Answers

Answer:

involuntary muscles can be defined as the muscles that functions or contracts without conscious control and are controlled by the autonomic nervous system.

Answer:

involuntary: A muscle movement not under conscious control e.g. the beating of the heart.

Other Questions
Allocating overhead cost to accomplish smoothing LO 12-2 Fanning Corporation expects to incur indirect overhead costs of $78,325 per month and direct manufacturing costs of $19 per unit. The expected production activity for the first four months of the year are as follows. January February March April Estimated production in units 4,700 8,800 3,800 6,800 Required Calculate a predetermined overhead rate based on the number of units of product expected to be made during the first four months of the year. Allocate overhead costs to each month using the overhead rate computed in Requirement a. Calculate the total cost per unit for each month using the overhead allocated in Requirement b. Allocate overhead costs to each month using the overhead rate computed in Requirement a. Month Allocated Cost January February March April Total Calculate the total cost per unit for each month using the overhead allocated in Requirement b. Month January February March April Number of units 4,700 8,800 3,800 6,800 Expected cost Overhead Direct costs Total cost Cost per unit Which of the following perceived barriers to divorce actually distinguishes couples who divorce from those who do not?A) Religious normsB) Fear of financial ruinC) Threat of losing one's childrenD) Worry that children will suffer You exert a force of a known magnitude F on a grocery cart of total mass m. The force you exert on the cart points at an angle below the horizontal. If the cart starts at rest, determine an expression for the speed of the cart after it travels a distance d. Ignore friction. Consider the thin plate shown in the sketch . Suppose that a = 170 mm, b = 450 mm, r = 50 mm. The material has a mass per unit area of 20 kg/m2.Determine the mass moment of inertia of the thin plate about an axis perpendicular to the page and passing through point O.a) 0.785 kg-m2b) 0.738 kg-m2c) 0.0273 kg-m2d) 1.20 kg-m2 people should be careful not to damage the electronic air sensor in their car.A. Conjunction B. DisjunctionC. ConditionalD. NegationE. Simple StatementF. None of the above The table shows Jerry's total utility from burgers and magazines. The price of a burger is $2, the price of a magazine is $4, and Jerry has $12 a week to spend. Burgers Magazines QuantityTot Quantity Total per weekutilityper week utility When Jerry maximizes his total utility, what quantities of burgers and magazines does he buy in a week? 28 48 64 76 200 240 268 Jerry maximizes his total utility when he buys burgers andmagazines a week. State Street Beverage Company issues $802,000 of 11%, 10-year bonds on Ma rch 31, 2017. The bonds pay interest on March 31 and September 30. Which of the following statements is true? A. If the market rate of interest is 12%, the bonds will issue above par B. If the market rate of interest is 12%, the bonds will issue at a premium. C. If the market rate of interest is 12%, the bonds will issue at pa. D. If the market rate of interest is 12%, the bonds will issue at a discount among the following, which is not known to represent a significant risk factor for a pregnancy being affected by a neural tube defect? social scientists generally agree that there are four components to the phenomena we label as ""feelings."" they are Which of the following are more likely to use subjective selection criteria to choose among competing computer products?Small organizations actual authority is the authority that the third party may reasonably assume that the agent possesses A(n) ______ matrix displays a model's correct and incorrect classification. a. decile-wise lift chart. b. cumulative lift. c. ROC curve. d. confusion. Determine whether the following statements are true or false:A. In the Keynesian Cross model, the slope of the planned expenditure line is the marginal propensity to consume.B. Joseph Schumpeter said that progress occurs through the process of saving and investment.C. According to the Solow model, an increase in the rate of population growth will reduce the output per worker.D. An increase in the interest rate will shift the IS curve to the left. Sales is in Units of $1000.00 and Advertising is in Units of $100. What does the value of sales INCREASE in dollars when we increased advertising by one UNIT? The equation is: Y=45.9+11,798.00X. Answer format: Number: Round to: 0 decimal places. Determine the molar solubility of mg(oh)2 in a solution buffered at a ph of 4.5. Evaluate the following integral using complex exponentials and write the result in complex exponential form. do not include the arbitrary constant. e^7x cos (x) dx a nurse is performing a head-to-toe assessment and is preparing to examine the client's ears. which equipment would the nurse need to have readily available? The polyvinyl chloride bar is subjected to an axial force of 900 lb. The angle decreases by = 0.01 after the load is applied. Epvc = 800(103) psi. (Figure 1) Part A If the bar has the original dimensions shown determine the value of Poisson's ratio. Express your answer using three significant figures. Which 3ways ways in which Soweto protestors harmed their community in their attempt to be public participants Matching the fictional setting to the tone makes sense, but it's also predictable.T/F