Biochemists working with proteins use a diverse set of treatments to analyze protein sequences. Denaturing agents unfold proteins. Some denaturing agents are reversible, and allow the proteins to be renatured after the procedure. A strong________ solution is a common reversible denaturant. Additional treatment with____________completely denatures proteins that contain disulfide bonds.

Answers

Answer 1

Answer:

Ethanol2-mercaptoethanol

See explanation below

Explanation:

Biochemists working with proteins use a diverse set of treatments to analyze protein sequences. Denaturing agents unfold proteins. Some denaturing agents are reversible, and allow the proteins to be renatured after the procedure. A strong ethanol solution is a common reversible denaturant. Additional treatment with 2-mercaptoethanol completely denatures proteins that contain disulfide bonds

Answer 2

A strong UREA solution is a common reversible denaturant. Additional treatment with 2-mercaptoethanol completely denatures proteins that contain disulfide bonds.

Denaturing reversible agents can alter the secondary/tertiary structure of a protein without altering its primary structure.

Urea is a reversible denaturant that breaks hydrogen bonds in the secondary structure of proteins without modifying the primary structure.

2-mercaptoethanol is a denaturing agent that cleaves disulfide bonds between cysteine residues.

In conclusion, a strong UREA solution is a common reversible denaturant. Additional treatment with 2-mercaptoethanol completely denatures proteins that contain disulfide bonds.

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Related Questions

Sarah swims at a speed of 6 meters/second and swims for 220 seconds, how far did she swim?

Answers

Answer:

1320m

Explanation:

6 times 220

1320

The graphic shows the largest fresh and salt water sources on Earth. Which of these sources BEST completes the graphic? A. Snow B. Clouds C. groundwater D. Swimming

Answers

Answer:

Explanation:

C

Label each region of the body with the function of the epithelium that is found there.

Absorption and
digestion

Olfactory mucosa
Filtration

Protection and
sensation

Sensation

For the following:

Skin

Glomerulus epithelium

Digestive epithelium

Olfactory mucosa

Answers

There for the answer is c

The small intestine serves as the site of both digestion and absorption. The small intestine contains a straightforward columnar epithelium.

The epithelium's tissue

The human body is made entirely of epithelial tissue. It supports a variety of bodily processes, including defense, secretion, absorption, and filtration.

A unique type of tissue called epithelial tissue can be found in the skin's outer layer.

The small intestine serves as the site of both digestion and absorption. The small intestine contains a straightforward columnar epithelium. The term "digestive epithelium" is sometimes used.

The pseudostratified columnar epithelium is present in the human olfactory mucosa. The skin serves as protection and contains epithelial tissue. The kidney contains glomerulus epithelium, which aids infiltration.

Therefore, the small intestine is where digestion and absorption occur.

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Answer please! have a great day/night

Answers

Answer:

meisos

Explanation:

because ots mutating

Answer:

meiosis is the nuclear division that forms sex cells

What is the image
of the point (3, 1)
translated 8 units
left and 3 units
up?

Answers

Answer:

(-5,4)

Explanation:

cuz u take 3-8 witch gives u -5 and u do 3+1 witch gives u 4

also u can take the long way and count backwards from 3


Which of the following BEST describes the recommended simple sugar intake for athletes?
A.Most athletes should eliminate all sugar from the diet.
B.Most athletes should keep sugar intake to less than 5% of total daily energy intake.
C.Most athletes should keep sugar intake to less than 10% of total daily energy intake.
D. Sugar should not be restricted in the athlete's diet because it provides a good source of carbohydrate for the working muscle.
.

Answers

Answer:

B

Explanation:

because sugar is common in food but he needs to limit it

Four friends were talking about how matter
and energy move through an ecosystem. This
is what they said:

Morrie: "I think only energy cycles through
an ecosystem

Feliciar "I think only matter cycles through
an ecosystem

Stefano: "I think both matter and energy
cycle through an ecosystem.

Lincoln: "I think neither matter nor energy
cycles through an ecosystem

Which friend do you most agree with?
Explain your
Thinking

Answers

Answer: Stefano

Explanation: Matter and energy changes forms.

Help I tried this last time and nobody answered the question

Answers

Answer:

It is option C probably.............

Most of the Earth's volume is contained in the _______ .
A.
mantle
B.
crust
C.
outer core
D.
inner core

Answers

The answer would be a mantle

Answer: Mantle

Explanation: I got it right on study island

The neuron that receives the neurotransmitters is called the?

Answers

Dendrites receive messages from other neurons. Axon sends messages from the cell body to the dendrites of other neurons.

what are the effector during the homeostasis of body temperature ​

Answers

Answer:

Body temperature is regulated by negative feedback. The stimulus is when the body temperature exceeds 37 degrees Celsius, the sensors are the nerve cells with endings in the skin and brain, the control is the temperature regulatory center in the brain, and the effector is the sweat glands throughout the body.

Swear glands is an example of an effector. An effector is an organ or cell that responds to a stimuli. Sweat glands release sweat to cool the body off when hot.

What are the first organisms to live within a newly created patch of land called?

Give examples


Ecology

Answers

Answer:

Pioneers

Explanation:

This question is related to primary succession, which is the first time colonization of a bare rock by certain organisms called PIONEER SPECIES. In this type of succession, a rock surface where there is absence of soil or vegetation is colonized by these set of organisms known as PIONEER.

These pioneer species greatly aid in the formation of soil in that area in order for other form of lives to inhabit the area. Examples of pioneer species that are adapted to grow on these bare or Rocky surfaces are LICHEN, GRASSES etc.

Which of the following occurs during transformation?


A pilus transfers genes between cells.


DNA is excised from one location to another within a DNA molecule.


Genes are transferred between cells in a virus.


Naked DNA is taken up from the environment.

Answers

B. DNA is excised from one location to another within a DNA molecule

Explanation:

B. DNA is excised from one location to another within a DNA molecule

Rocks from which source would most likely contain the highest percentage of silicate
minerals?
1. Ocean Basin
2. Volcanic Island
3. Mid ocean Range
4. A mountain in the middle of a continent

Answers

Answer:

The correct answer is (D): A mountain in the middle of a continent.

Explanation:

Understand the difference between atom, molecule, salt (or ionic compound), and
compound

Answers

Answer:

Whether something is a molecule or not depends on the type of bond that is formed when its atoms join together. ... Something like table salt (NaCl) is a compound because it is made from more than one kind of element (sodium and chlorine), but it is not a molecule because the bond that holds NaCl together is an ionic bond

How long can blood flow to the brain be interrupted before death occurs?

Answers

Answer:

Brain cells start to die if they go without oxygen for just 3-4 minutes and that's exactly what happens during a stroke.

Explanation:

Stroke is an abrupt interruption of constant blood flow to the brain that causes loss of neurological function. The interruption of blood flow can be caused by a blockage, leading to the more common ischemic stroke, or by bleeding in the brain, leading to the more deadly hemorrhagic stroke.

Brain death can occur rapidly as cells begin to die off without any oxygen stores or new oxygen circulating to the brain.

Which of the following is most damaging to the ozone layer?
carbon dioxide
methane
O Oxygen
chlorofluorocarbons

Answers

Answer:

Chlorofluorocarbons...............

Answer:

chlorofluorocarbons

Explanation:

chlorofluorocarbons are mainly responsible for man-made chemical ozone depletion.

What are the two most important neurotransmitters and why ?

Answers

Answer:

The most common neurotransmitter is acetylcholine, which often is the messenger between axons and muscles as well. Other common neurotransmitters are octopamine, serotonin, and dopamine; they usually function in the central nervous system. All of these neurotransmitters are found in both vertebrates and invertebrates.

Explanation:

Which solid waste has the highest percentage in terms of not being recycled?

A)

paper

B)

plastics

C)

food scraps

D)

metals

Answers

Answer:

plastic

Explanation:

Answer:

plastics

Explanation:

8.7% recycled as opposed to 47% of metal and 80 something% of paper.

If a protein will end up with 30 amino acids, how many codons, not including the STOP codon, were on the mRNA
transcript that produced it?

Answers

Answer:

30 codons

Explanation:

Translation is the second process of gene expression, which involves the synthesis of amino acid sequence from mRNA. This process occurs in the ribosome where the mRNA is read in a group of three nucleotide bases called CODON. Each codon specifies a particular amino acid.

Since one codon specifies or translates into one amino acid, a protein with 30 amino acids will contain 30 codons (exclusive of STOP CODONS) on the mRNA transcript.

Please help ASAP pleas

Answers

Answer:

Option c is Ur answer have a great day ahead

choose the correct one, please

Answers

Answer:

I believe it is called mitosis

Explanation:

what should you be thinking of before designing your research​

Answers

Answer:

well it depends

Explanation:

you need to use keywords when researching terms or questions and be ready to read.

One parent has blood type O, and the other has blood type Bi. What is the chance that they have a child that is blood type O?

Answers

Answer:

50%

Explanation:

Draw a Punnett square to solve this problem (see photo).

Blood type O has two recessive alleles, represented by two lowercase “i”s.

Blood type Bi has a dominant allele shown by capital i with superscript B and a recessive allele “i”.

In the result, two boxes are “IB i” (for blood type Bi) and two boxes are “i i” (for blood type O).

The chance of having a child with blood type O is:

2/4 = 1/2 = 50%

Therefore, the chance of having a child with blood type O is 50%.

Answer:

50

Explanation:

2/4 = 1/2 = 50%

NS is a 32-year-old female who had been sick with a cold virus for 10 days. She states that she had flu symptoms of cough, sore throat, and fatigue. It has been 3 weeks since the cold, and she continues to be fatigued and finds it hard to get enough energy to do things. NS says she has noted that she has been bruising easily. She hit her arm accidentally on the edge of a counter, and now she has a large bruise. She has also noted little purple spots all over her hands and chest. On exam it is noted that she does have a large bruise on her right arm and petechiae and purpura over her chest and hands. Lab work comes back with a platelet count of 40,000mm^3.

Required:
NS has been diagnosed with idiopathic thrombocytopenic purpura (ITP). Explain what it is and who can get it.

Answers

Answer:

Idiopathic thrombocytopenic purpura or ITP is also known as Immune thrombocytopenic purpura that is a disorder in which there is an abnormality in the clotting of blood due to a decreasing number of platelets in an individual.

Platelets plays role in clotting the blood to prevent bleeding so this disorder leads to easy bruising, bleeding gums, and internal bleeding. It is an autoimmune disorder as in this case, an immune reaction takes place against one's own platelets.

It occurs in all age and sex groups however, it occurs normally in young children and females more often.

What happened NEXT after ice cutters made ice blocks?

Answers

Answer:

the blocks were transported to ice houses

Explanation:

i got it right

Explain events which take place during dark reaction​

Answers

Answer:

carbon-fixing

Explanation:

Dark reaction is also called carbon-fixing reaction. It is a light-independent process in which sugar molecules are formed from the carbon dioxide and water molecules. Plants capture the carbon dioxide from the atmosphere through stomata and proceed to the Calvin cycle. ...

What is a tree that produces cones called?

Answers

Answer:

Gymnosperms

Explanation:

Plants w/ cones

Which of the following correctly describes the relationship of different organisms in an ecosystem? A. All animals consume plants as their only energy source. B. Some animals receive energy from plants, while others receive energy from other animals. C. Plants and animals both receive energy from the Sun that they use to make food. D. All plants depend on animals as their only energy source.

Answers

Answer:

Plants depend on the sun because of the energy it provides them.

Explanation:

Answer: plant depend

Explanation:

how do all of the parts of the cell help to make it function as a whole?

Answers

in many ways

Explanation:

cells is composed of many parts as it formed cell

Because all cells are together
Other Questions
At which values of x does the graph of the function F(x) have a vertical asymptote? Check all that apply. F(x)= x-5 / x^2 + 3x -18 The graph shows the location of P in point hour. Point are is on the Y axis and has the same Y coordinate of point PPoint Q is graft at (N, -2). The distance from P to Q is equal to the distance from P point R. What is the distance from point P to point Q? What is the value of N? Explain how you determine the distance from point B to point Q, and the value in. The graph shows the location of P in point R. Point are is on the Y axis and has the same Y coordinate of point P Which function shows a positive rate of change? How many of the offspring are heterozygous? 4, 20, 100, 500, whats the next number? How many grams are there in 0.250 moles of Ba(NO3)2? What is the value of z? which example is a valid restriction of free speech A: A government agency censors a controversial newspaper before it is printed. B. a religious leader is stopped from calling her fellow worshipers to prayer on the radio C. person is arrested for yelling fire in a crowded movie theatre as a practical joke D. students are arrested for criticising government policies as the press conference Suppose Nationwide increases the insurance premium they charge for their auto policies by 6 percent. In response, the demand for State Farm auto policies in a small town increases from 1,500 to 1,650. What is the cross-price elasticity of demand for State Farm auto policies in this town?Using the midpoint formula, the cross-price elasticity of demand for State Farm auto policies is _____. (Round to 3 decimal places.)In this instance, auto insurance from Nationwide and auto insurance from State Farm are _____. Which statement accurately reflects the similarities between the Aztecs and the Incas Two sisters want to save money to buy a game. The game costs $90. One sister has $36 and the other has $40. How much more money do they need? Excerpt taken from The Historic Rise of Old Hickory by Suzanne B. WilliamsFour major candidates ran in the 1824 election, all under the "Democratic-Republican" name. One of the candidates, Andrew Jackson, was already famous. In the 1780s, he earned the right to practice law and served in various offices of the state government, including senator. He earned the nickname "Old Hickory" for his toughness as a general during the War of 1812 and First Seminole War. Jackson supported slavery and "Indian removal." This earned him support from voters in southern and frontier states. The other three candidates were John Quincy Adams of Massachusetts, Henry Clay of Kentucky, and William Crawford of Georgia.U.S. presidents are elected through the Electoral College. The Founding Fathers worried that Americans were too spread out to learn enough about the candidates. Under the Electoral College, Americans cast their ballot for the popular vote, which chooses the electors for each state. The number of electoral votes each state equals the number of representatives and senators combined. The candidates must win an absolute majority of electoral votes to win the election.In 1824, Andrew Jackson won the popular vote, but he did not win it in each state. Jackson and Adams both won many electoral votes. Jackson won the most with 99. However, a candidate needs an absolute majority of electoral votes to win. In 1824, Jackson needed 131 to win. When there is not majority winner, the election goes to the House of Representatives. This has only happened twice in U.S. history.Even though he won the popular vote and many electoral votes, Andrew Jackson lost the presidency in 1824. John Quincy Adams was the Secretary of State at this time. Henry Clay was the Speaker of the House of Representatives. Henry Clay, receiving the least, was left out. However, as a leader in the House of Representatives, he had influence over the other members. Clay openly hated Jackson and there were rumors that Clay made a deal with Adams in exchange for his support. The House election declared John Quincy Adams president. Soon, he chose Henry Clay to fill the seat he left vacant, Secretary of State. Jackson was shocked and enraged. Although there was no inquiry of possible wrongdoing, Jackson accused Adams and Clay of making a "corrupt bargain."John Quincy Adams was a disappointment as president. Many of his goals created divisions like federal funds for internal improvement. Some states thought that taking federal funds would force them to follow certain rules. They felt this reduced their rights as independent states. Jackson took advantage of issues like this one to gather more support. More Jackson supporters found their way to seats in Congress. He was as a man of the people and said Adams could never understand the common man's concerns.John Quincy Adams ran against Andrew Jackson in the 1828 election. Personal attacks grew even more vicious, but Andrew Jackson appealed to many. He believed government was for the common man. He believed in strict reading of the law and limited internal improvements. He also believed in states' rights.Andrew Jackson easily won the 1828 election, winning both the popular vote and a majority of electoral votes. Historians note the sectional nature of the voting. Support for Jackson was concentrated in South while Adams' support was mostly in the North. Jackson was so popular because he brought changes to the government. He also wanted to make sure the government was responsible for its actions. Jackson pushed settlement into the frontier. He supported the Indian Removal act. He also defended the spread of slavery. Though his support was heavier in the South, he was determined to keep a unified nation. The rise and presidency of Old Hickory is memorable to Americans today.Which of the following did Andrew Jackson not support? (5 points) aA limited national government bSpreading of slavery to the frontier cFederal funds for internal improvements dA strict interpretation of the U.S. Constitution Find the exact value of x. Round to the nearest tenth. Roberto and Reagan are both 25-percent owner/managers for Bright Light Incorporated. Roberto runs the retail store in Sacramento, California, and Reagan runs the retail store in San Francisco, California. Bright Light generated a $131,300 profit companywide made up of a $76,800 profit from the Sacramento store, a ($29,500) loss from the San Francisco store, and a combined $84,000 profit from the remaining stores. If Bright Light is taxed as a partnership and it is decided that both Roberto and Reagan will be allocated 70 percent of his own store's profit, with the remaining profits allocated pro rata among all the owners, how much income will be allocated to Reagan in total Solve for X in 4^x + 6^x = 9^x What is the value of "twelve more than the quotient of a number and two, decreased by four" when n = 4? There were a total of 480 tickets sold for the school play. Adult tickets sold for $8 and student tickets sold for $5. The ticket sales brought in $3369. How many adult tickets and student tickets were sold? Square ABCD is shown below.If BD = 8x - 50, and DE = 2x + 5, find BE.A) 15B) 35C) 25D) 45 Here is a list of numbers13 14 18 2318 23 30 36From the numbers in the list, write down the multiple of 4 and the factor of 28 Given the following trapezoid, if QR=8 and UT=12, find the length of the midsegment VS?