Only cells that express a puromycin-resistance (PR) gene can grow in the presence of the drug puromycin. Ganciclovir is a nontoxic drug that is converted to a lethal toxin in a cell that expresses thymidine kinase (TK). Thy-1 is a fibroblast-specific marker protein. Which of the following selection strategies is the most reasonable one to use in experiments that attempt to generate induced pluripotent stem cells (iPS cells) from fibroblasts (1 point, letter)? Explain your choice (4 points, 2-3 sentences).
Engineering fibroblasts to express PR under the control of the Nanog promoter, and selecting iPS cells in puromycin-containing media
Engineering fibroblasts to express PR under the control of the Thy-1 promoter, and selecting iPS cells in puromycin-containing media
Engineering fibroblasts to express TK under the control of the Nanog promoter, and selecting iPS cells in ganciclovir-containing media
Engineering fibroblasts to express TK under the control of the Thy-1 promoter, and selecting iPS cells in ganciclovir-containing media
Engineering fibroblasts to express TK under the control of the Thy-1 promoter, and selecting iPS cells in puromycin-containing media

Answers

Answer 1

The most reasonable selection strategy to use in experiments that attempt to generate induced pluripotent stem cells (iPS cells) from fibroblasts is engineering fibroblasts to express PR under the control of the Nanog promoter, and selecting iPS cells in puromycin-containing media (Option A).

Nanog is a transcription factor that plays a critical role in maintaining pluripotency, and therefore selecting for cells that express PR under the control of the Nanog promoter ensures that only cells with high pluripotency potential will survive in the presence of puromycin. This approach ensures that only cells with pluripotent properties, as indicated by Nanog expression, will survive in the presence of puromycin, while cells expressing fibroblast-specific markers, like Thy-1, will be eliminated.

Thus, the correct option is A.

Learn more about puromycin: https://brainly.com/question/14351857

#SPJ11


Related Questions

How are all animal cells alike?

Answers

Answer:

Animal cells have centrosomes and lysosomes

Can someone help me fill in the blanks. Thank you.​

Answers

Answer:

1) fungi

2) tubers

3)bulbs

4) unequally

5) cuttings

6)equally

7)root

8) mitosis

this my knowledge on this questions...hope i helped!

When there is a constant unbalanced force applied in the opposite direction as the motion, speed will ___________________ and acceleration will ________________________.

Answers

Answer:

When there is a constant unbalanced force applied in the opposite direction as the motion, speed will decrease and acceleration will increase.

Explanation:

An opposing force should slow something down, decreasing the speed, and acceleration is just change in speed or direction.

Have a great day!

Please help me with this biology question...........


Chloroplasts are present in some plant cells only Give an example of plant cells that do not contain chloroplasts and explain why​

Answers

Answer:

Chloroplast is specific to plant cell because plants are the autotrophs which can prepare their food by conversion of light energy to chemical energy. It is the vital photosynthetic pigment in leaves as it involves the conversion of light energy into chemical energy.

hope it is helpful.

Answer:

Please let me no if you still need help on this

Explanation:

I will glady help!

The picture shows an organ system in the human body
Brain
Nerve
-Spinal
cord
w
do these organs do in the human body?
O A. They filter pathogens and dead cells out of lymph before it returns
to the blood.
B. They monitor and react to changes in the body and its
environment
C. They release hormones that control the activities of cells and
organs
O D. They identify and destroy things that are foreign or harmful to the
body

Answers

Answer:

B

Explanation:

because the nerval system is for sending and capturing signals from the brain to monitor if you had a broken bone so your brain and nerves say you are feeling pain. on rare occasions the nerves will totally skip the pain part and send different information to your brain, like if you broke your finger, it would skip the pain and all you would see is a weird finger.

Yes it’s b Nice one

DNA sequences can act as "tape measures of evolution." Some highly-conserved regions of the human genome (similar to comparable regions in other species) don't code for proteins. Why?
1. Such regions coded for proteins in genomes of distant human ancestors, but over time they became noncoding; however, conserving their sequence remained unchanged.
2. Such regions play a significant role in gene regulation. That is why they remain conservative under the influence of natural selection.
3. Such regions are the sites of DNA recombination. They should remain conservative to provide an accurate recombination process during cell division.
4. Such regions remain conservative because the mutation rate differs along the genome. In other words, such regions remain conservative accidentally.

Answers

Answer:

2. Such regions play a significant role in gene regulation. That is why they remain conservative under the influence of natural selection.

Explanation:

Within the genome there are many non-coding regions that control the expression of master developmental genes such as, for example, Hox major transcription factors (TFs) in animals and MADS-box TFs in plants. These gene regulatory regions (e.g., promoters and enhancers) are generally evolutionarily conserved within certain taxonomic groups. In consequence, such non-coding regions are well known to be conserved and interact in a sequence-specific manner with major transcription factors in order to regulate developmental pathways.

Would the answer to this be C or would it be A?

Answers

Answer:

I'm thinking maybe A

Explanation:

A football is kicked by a player. The ball travels 50 m, to the west when it is caught by another player and that player travels 50 m, south. Create a vector diagram that shows both displacement vectors and the resultant displacement vector of the football.Use the scale of 1 cm of the graph paper = 10 m of motion.

Answers

Answer:

The football will hit the ground 2.11 seconds later.

Explanation:y = y0 + v0yt + ½ayt

2

0 = v0sin  t + ½ayt

2

0 = t(v0sin + ½ayt)

t = 0 or v0sin + ½ayt = 0

½ayt = – v0sin

=

−20sin

=

−2(18.0)sin(35.0°)

−9.8

Scientists determined that an unknown organisms was a eukaryote and not prokaryote. Which characteristic would have allowed them to make this determination?


Grupo de escolhas da pergunta


A) Nuclear envelope


B) Cell wall


C) DNA


D) Flagella




ASAP

Answers

Answer:

cell wall would have allowed them to make this determination

Scientists would have determined that an unknown organism was a eukaryote and not a prokaryote based on the presence of a nuclear envelope (Option A).

What is a nuclear envelope?

The nuclear envelope is a double-membrane structure that surrounds the nucleus in eukaryotic cells and separates the genetic material (DNA) from the rest of the cytoplasm. Prokaryotes, on the other hand, do not have a distinct nucleus or a nuclear envelope. Instead, their genetic material is dispersed throughout the cytoplasm and is not separated from the rest of the cell

Options B, C, and D (cell wall, DNA, and flagella) could also be used to determine whether an organism is a prokaryote or eukaryote, but the presence of a nuclear envelope is a key characteristic that definitively separates the two types of organisms.

Learn more about nuclear envelope, here:

https://brainly.com/question/13334437

#SPJ6

Kerstin is getting ready to graduate high school. She wants to become a cardiac perfusionist. Which best describes the path she should take to her career?

Answers

Answer:

four-year degree , master’s degree , certification exam from ABCP

Explanation:

Answer:

She should do a four-year degree , master’s degree , certification exam from ABCP

Explanation:

SOMEONE PLEASE HELP ME WITH THIS I REALLY NEED HELP I WILL GIVE BRAINLY IF U GET IT RIGHT

Answers

im pretty sure it’s c

I would say C is the answer

How are the chromosomes sorted during meiosis

Answers

Answer:

During meiosis l, homologous chromosomes pair up with each other.When the homologs are divided into daughter cells,the chromosomes are sorted randomly into daughter cells.if alleles of different chromosomes,then they assort indepently

How do individual’s affect environmental systems?

Answers

Answer:

Humans impact the physical environment in many ways like overpopulation

Explanation: pollution, burning fossil fuels, and deforestation. Changes like these have triggered climate change, soil erosion, poor air quality, and undrinkable water. and many more ways.

Answer:

Humans impact the physical environment in many ways like overpopulation

Explanation:

pollution, burning fossil fuels, and deforestation. Changes like these have triggered climate change, soil erosion, poor air quality, and undrinkable water. and many more ways.

Anyone know this question?​

Answers

won’t let me do it hold on

Answer:

Its below in the explanation

Explanation:

the

AA is Hh

Bb is Hh

Cc-Hh

Dd-Hh

Ee-Hh

ff-HH

GG-HH  

HH-HH

ii-Hh

Jj-Hh

kk-HH

Ll-Hh

Mm-Hh

nn-HH

oo-HH

Pp- Hh

Government agencies regulate most aspects of agricultural safety.
True
False

Answers

Answer:

False

Explanation:

Based on your knowledge of the relationship between CO2 and photosynthesis explain why CO2 cycles throughout the year.

Answers

Answer:

we breath in Oxygen and breath out CO2 and CO2 destroys the atmosphere

As a cell grows in size, transporting the materials in and out of the cell becomes more difficult. Which process would help a cell to efficiently transport more materials into the cell as it grows?
Grupo de escolhas da pergunta

A) The cell develops a thinner cell membrane.

B) The cell undergoes a mutation.

C) The cell stores more materials in its vacuoles.

D) The cell divides into two cells creating more space for materials.


ASAP

Answers

Answer: D

Explanation: I answered the question

When the process would helps a cell to efficiently transport more materials into the cell as it grows: The cell divides into two cells creating more space for materials.

How does a Cell Grow?

When a Cell growth is not to be confused with cell division or the cell cycle, which are distinct processes but they can occur alongside cell growth during the process of cell proliferation,

whereas a cell, it is known as the "mother cell", Also, when grows and divides to produce two " daughter cells ".

Therefore, The correct option is 'D'. The cell divides into two cells creating more space for materials.

Find more information about Cell Grow here:

https://brainly.com/question/26426027

Which is the primary source of stem thickening in plants? xylem vascular cambium internal phloem

Answers

The primary source of stem thickening in plants is the vascular cambium. It is a plant tissue located between the phloem and the xylem and in the root of a vascular plant. It is the source of secondary growth or the radial growth of the stem of a plant.

Describe how proteins form (polypeptide)

Answers

Answer: Atoms were created after the Big Bang 13.7 billion years ago. As the hot, dense new universe cooled, conditions became suitable for quarks and electrons to form. Quarks came together to form protons and neutrons, and these particles combined into nuclei.

Answer:

Within a protein, multiple amino acids are linked together by peptide bonds, thereby forming a long chain. Peptide bonds are formed by a biochemical reaction that extracts a water molecule as it joins the amino group of one amino acid to the carboxyl group of a neighboring amino acid.

Explain why society has been developing different renewable resources in favor of
nonrenewable resources in the past few decades.

Answers

Answer: Society has been developing different renewable resources in favor of nonrenewable sources in the past decades because natural gas and oil has been used to much that we are running out of nonrenewable sources. So society has been trying to use solar panels, wind panels, and water.  

Explanation:

SOMEONE PLEASE HELP ME WITH THIS I REALLY REALLY REALLYYY NEED IT ILL GIVE BRAINLY IF U GET IT RIGHT

Answers

Answer:

i like trains and turtles

Explanation:

If a middle level species were removed from the community, how might the flow ofenergy be affected? Explain your answer.

Answers

There will be far-reaching effects. If for example, the producer is removed, the consequences could be dire. Producers capture sunlight directly and make chemical energy for consumers. If this is the only producer that a particular consumer eats, it may die as well.
518 views





Related Questions (More Answers Below)

Please help me, really need it

Answers

Answer:

they r more sever for them because they r a mixed blood type

sumtimes it is very rare for anyone of a mixed blood type to be more vunarable to a diesise rather than a full a or b

Explanation:

Answer:

I believe it is because having two x chromosomes gives the genome a fail safe as it can use the other x chromosomes' non-mutated sequences

Explanation:

1. What do DNA Replication and Cell Division have in common?
2. Define Cell Differentiation in your own words.
3. Why are stem cells consjdered unique?

Answers

Answer:

1: During every cell division, a cell must duplicate its chromosomal DNA through a process called DNA replication. The duplicated DNA is then segregated into two "daughter" cells that inherit the same genetic information. This process is called chromosome segregation.

2: Cellular differentiation is the process in which a cell changes from one cell type to another. Usually, the cell changes to a more specialized type. Differentiation occurs numerous times during the development of a multicellular organism as it changes from a simple zygote to a complex system of tissues and cell types.

3: Stem cells have unique abilities to self-renew and to recreate functional tissues. Stem cells have the ability to self-renew. Unlike muscle cells, blood cells, or nerve cells—which do not normally replicate— stem cells may replicate many times.

Explanation:

Goooooooooooooooooogle <3

Magma is molten (liquid) rock under Earth’s surface. Based on the image, how do you think magma turns into extrusive igneous rock?

Answers

Answer:

Magma come out of volcanoes that cools down and later on hardens up into rocks.  

Explanation:

Magma come out of volcanoes that cools down and later on hardens up into rocks.

What is Magma?

The existence of magma is essential to comprehending the geology and morphology of the mantle because the majority of the mantle and crust are solid.

Different types of magma form as a result of variations in the mantle's and crust's structural features, pressure, and temperature. Decompression Melting The mainly solid mantle of the Earth moves higher during decompression melting.

Convection causes this hot material to rise to a place with lower pressure.

Therefore, Magma come out of volcanoes that cools down and later on hardens up into rocks.

To learn more about magma, refer to the link:

https://brainly.com/question/16940653

#SPJ2

Tides are caused by gravity of the__
1. Earth and sun
2.earth and moon
3.moon and stars
4.sun and moon

Answers

Answer:

the answer to your question is number 2

When we doing Strawberry Extraction, why do we need to remove the air as much as possible before seal the bag?

Answers

to keep it preserved and delicious

Answer:

Why do we need to cool the mixture? DNases or restriction enzymes that destroy DNA are present in the cell's cytoplasm. They are there to protect the cell from invasion by viruses. Once the nuclear membrane is destroyed by the soap, the DNA is now susceptible to the DNases and will quickly be degraded.

Explanation:

When you added the salt and detergent mixture to the smashed strawberries, the detergent helped lyse (pop open) the strawberry cells, releasing the DNA into solution, whereas the salt helped create an environment where the different DNA strands could gather and clump, making it easier for you to see them.

Germs that cause diseases?

Answers

Answer:

There are two main types of germs which can cause disease in humans and animals. These are bacteria and viruses. Bacteria are larger than viruses.

Explanation:

A cell containing 20 chromosomes undergoes mitosis, each daughter cell will have 20 chromosomes
a.True
b.False

Answers

Answer:

the answer is true because a cell containing 20 chromosomes undergoes mitosis, each daughter cell will have 20 chromosomes

PLEASE HELP IM DOING A TEST RN!!
Which of the following describes how the pollen must travel in order for pollination to occur?

Question 18 options:

. from the stigma to the anther


from an anther to the style


from an anther to the stigma


from the stigma to the pollen tube

Answers

Answer:

from an anther to the stigma

Explanation:

Answer:

Anther to stigma!

The anther is where the pollen is granulated, and the stigma is the part where they reproduce.

Other Questions
PLEASE HELP 50 POINTS Which terms can be combined in the expression below? Select three options.StartFraction 5 over 6 EndFraction a minus 2 b minus 16.3 + StartFraction b over 12 EndFraction + c minus 0.9 bStartFraction 5 over 6 EndFraction a2b16.3StartFraction b over 12 EndFraction0.9b 1. What are the different properties of light that apply to the image formation ofoptical devices such as mirrors and lenses? En un modelo de un castillo, 1 pulgada equivale a 83 pies. El modelo mide 10.5 pulgadas de ancho. Cual es el ancho real del castillo?Aydenme porfavo ;w; in estimating the body temperature of a person, what difficulties did you encounter?Explain PLZZZZZ HELP ME! Its math btw i need the Birthplace and date of birth of Louis Pasteur please someone help me with this answer please !! *PLEASE ANSWER!!, THANK U*Why did War Hawks want the United States to go to war with Britain in 1812?a.) They wanted the United States to become part of the British Empire.b.) They felt it would strengthen the nation's manufacturing and shipping industries.c.) They believed it would stop the Indian raids along the American frontier. 1. Um prmio em dinheiro foi dividido entre 3pessoas: a primeira recebeu 1/4 do valor doprmio a segunda recebeu 1/3 e a terceiraganhou R$ 1000,00. Ento, o valor desse prmio,em reais, era de:a) 2400,00b) 2200,00c) 2100,00d) 1800,00 Help..!! Right answers will be marked as brainliest..! Answer all the questions..! f(x)= 10x find the inverse of the function Please help me with this homework Find the maeasure of the exterior angle in the figure PLEASE HELP ME!!!!What would happen to the members of a food web if most of the trees died? A. only the trees will be effectedB. only things that eat tree leaves will have less to eatC. Nothing will happenD. The whole food web will be disrupted If the available number of valence electrons for a molecule or polyatomic ion does not complete all of the octets in a lewis structure what should you do how much will $5000 be worth in 5 years if it is compounded continuously at 3% interest. What were the TWO parties formed from the split of the Democratic-Republicans?Liberal PartyFederalist PartyDemocratic-Republican PartyNational Republican PartyAmerican Party A object falls at 16t^2 feet in t seconds you drop a rock from a bridge that is 906 what is the hight in feet after the rock is droped Find the slope of the line I dont really understand this question, can anyone help me out?