To approximate the actual concentration of enzymes in a bacterial cell, assume that the cell contains equal concentrations of 1,000 different enzymes in solution in the cytosol and that each protein has a molecular weight of 100,000. Assume also that the bacterial cell is a cylinder (diameter 1.0 m, height 2.0 m), that the cytosol (specific gravity 1.20) is 20% soluble protein by weight, and that the soluble protein consists entirely of enzymes. Calculate the average molar concentration of each enzyme in this hypothetical cell.

Answers

Answer 1

Answer:

2.4 × 10⁻⁶ M

Explanation:

Molar concentration = no of moles of solute (enzymes)/volume of solvent(cystosol)

Let V = volume of solvent = volume of cystosol = πd²h/4 where d = diameter of cell = 1.0 m and h = height of cell = 2.0 m

Concentration of enzymes, C = n/V where n = no of moles of enzymes

Also, n = m/M where m = mass of all enzymes and M = molecular weight of protein = 100,000

Note that the solvent for the enzymes is cystosol, since they are dissolved in it.

Density of cystosol,ρ = specific gravity of cystosol in g/cm³ = 1.20 g/cm³.

Now, ρ = m'/V where m = mass of cystosol and V = volume of cystosol

m' = ρV

m' = 1.20V

Given that 20 % of the cystosol is protein and all protein are enzymes, the mass of enzymes is thus m = 0.2m' = 0.2 × 1.20V = 0.24V

C = n/V

= m/MV

= 0.24V/100000V

= 2.4 × 10⁻⁶ mol/cm³

= 2.4 × 10⁻³ mol/dm³

= 2.4 × 10⁻³ M

This is the concentration of all the 1000 enzymes.

So, the concentration of one enzyme is 2.4 × 10⁻³/1000 M = 2.4 × 10⁻⁶ M


Related Questions

Clearly outline what differentiates Gram +ve bacteria from Gram -ve bacteria?​

Answers

Answer:

Gram positive bacteria have cell walls composed of thick layers of peptidoglycan. Gram positive cells stain purple when subjected to a Gram stain procedure. Gram negative bacteria have cell walls with a thin layer of peptidoglycan.

Why can grasses not survive on the island yet?​

Answers

why can grasses not survive on what island?
Because it’s dry? I don’t know but I think it’s common sense

Please help me!

Explain how some structures in the skin protect your body.

Answers

The primary function of the skin is to act as a barrier. The skin provides protection from: mechanical impacts and pressure, variations in temperature, micro-organisms, radiation and chemicals.

8.
Waste products that are easily broken down by nature are

hazardous
toxic
biodegradable
recyclable

Answers

The answer to this question is Biodegradable

The total number of chromosomes in an organism is referred to as the
Will mark brainliest

Answers

Answer:

ploidy

Explanation:

Ploidy is the number of complete sets of chromosomes in a cell, and hence the number of possible alleles for autosomal and pseudoautosomal genes.

which is true of the gallbladder?

- it creates white blood cells
- it stores bile
- it stores pancreatic amylase
- it stores pepsin

Answers

Answer:

It stores bile is the true of the gallbladder.

But if the male Himalayan rabbit's genotype is c(h)c, and it mates with an albino female whose genotype is cc isn't there a 25% chance that the offspring could be albino? and why can't you breed two Himalayan's together to get a Himalayan?

Answers

Answer: Go-ogle

Explanation: Is helpful

what is monosachrayde​

Answers

Basically any type of sugar (glucose for example) that cant be broken down to a simpler sugar

Answer:

A monosaccharide is the most basic form of carbohydrates. Monosaccharides can by combined through glycosidic bonds to form larger carbohydrates, known as oligosaccharides or polysaccharides.

Explanation:

A monosaccharide is a type of monomer, or molecule that can combine with like molecules to create a larger polymer.

6. If a DNA sequence reads TCCA, a deletion mutation would look like...
a. TCTA
b. TCA
C. TCCCA
d. UCCA
I
I

Answers

Answer: C

Explanation: It would be C because a deletion mutation involves the loss of genetic material, therefore C is the only option that fits the definition.

Hope this helps

Explain the process sleet goes through from the time it leaves the clouds to the
time it hits the ground

Answers

Answer:

Sleet is simply frozen raindrops and occurs when the layer of freezing air along the surface is thicker. This causes the raindrops to freeze before reaching the ground. In addition, ice caused by freezing rain can rapidly add weight to tree branches and power lines, causing them to snap or break

Explanation:

Photo synthesis is impossible at a depth of 1000 b/c ? A. It is too hot B. Only blue light penetrates this far C. No light penetrates this far D. It is too cold​

Answers

Answer:

B. Only blue light penetrates this far

Explanation:

A segment of mRNA has the sequence UGACAUAGC. Which of the following would represent the
strand of DNA from which this piece of mRNA was made?
O ACTGTATCG
O ACUGUAUCG
O CGAUACAGU
O GTACAGATT

Answers

Answer:

B

Explanation:

U(uracil) pairs with A(adenine), G(guanine) pairs with C(cytosine)

Please anyone ASAP help

Answers

If I’m correct, it’s c

Answer:

C. There is a 25% chance of short offspring.

Explanation:

In the simplified genotypes of a Punnett square cross, we deal with two genotypes. In this case, we are looking at a cross between Tt and Tt. T is dominant and t is recessive, meaning that someone with any genotype with T in it will be tall.

TT, Tt, and Tt will all produce tall offspring, while tt will produce short offspring.

This means there is a 75% chance of tall offspring, or 3:4.

50% or 2:4 are expected to have the Tt genotype.

Only 1 of the 4 will produce short offspring, which is 25%.

How does being really cold affect the nervous system?

Answers

If the temperature is too hot, you may feel tired and lethargic, and if it is too cold, this may heighten the pain you feel.

In South Carolina, there is an area of shoreline called Grand Strand. Its central part is not protected by barrier islands. Explain what you could infer about the impact that water movement has on this area.

A. Water movement has caused strong waves to build the shoreline up through the process of deposition.

B. Water movement has caused longshore currents to erode the sediment from one end of this shoreline.

C. Water movement has caused this land to erode because there is no protection from strong waves.

D. Water movement has caused a build-up of various inlets to form a combination of tides and currents.

Answers

The impact that the movement of the water has in this area, is that the movement of the water has caused the erosion of this land because there is no protection against strong waves.

What causes marine erosion?

Marine erosion, also called maritime erosion, is defined as “the process of wear, transport and sedimentation of rocks and coastal soils by erosive agents”. Fundamentally, it can be caused by the action of three factors:

WavesCurrents and Tides.

With this information, we can conclude that the impact that the movement of water has in this area is that the movement of water has caused erosion of this land because there is no protection against strong waves.

Learn more about  water movement in brainly.com/question/16630851

Which skull bone is part of the facial bones and contains teeth?

Answers

Answer:

The anterior skull consists of the facial bones and provides the bony support for the eyes and structures of the face. This view of the skull is dominated by the openings of the orbits and the nasal cavity. Also seen are the upper and lower jaws, with their respective teeth

The anterior skull. I learned it in school plus i searched it up to make sure.

Why is Robert Llewellyn referred to as a seed artist?
Type here...
Use two adjectives that describe Robert.
Type here...

Answers

Explanation:

Robert is known as a "seed artist," because his pictures involved "growing" plants and "developing" seeds...

INTERIM K12 SCIENCE

HERE ARE ALL YOUR AWNSERS

K12 ELA 7th grade science interim


answers are labeled as numbers and letters a b c and d (1c 2d 7a 9b etc)

1A

2C

3D

4C

5B

6B

7B

8A

9D

10C

11D

12C

13C

14B

15B

16B

17A

18A

19C

20C

21B

21B

23B

24C

25B

I FEEL VERY GUILTY AND BAD

YOUR WELCOM

WHO EVER SEES THIS MUST SPREAD IT LIKE HOT BUTTER

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Lol☠️☠️☠️☠️☠️☠️☠️☠️☠️☠️

What is RNA? What similarities does it have to DNA?

Answers

Similarities Between DNA and RNA

Both DNA and RNA store genetic information. DNA and RNA are both large biological polymers. Both DNA and RNA consists of sugar, nitrogenous bases, and a phosphate backbone. On both molecules, guanine and cytosine pair with each other (are complementary).

Explanation:

An organ ________ is a combination of several organs that perform together for a common function. a. basic b. cells c. living d . molecules e. organ f. organism g. system h. tissue​

Answers

Explanation:

System is a combination of several organs that perform together for a common function.

Hope it will help :)❤

Pairs of chromosomes contain (the same/different) types of genes.

Answers

Answer:

The 22 pairs of homologous chromosomes contain the same genes but code for different traits in their allelic forms since one was inherited from the mother and one from the father. So humans have two homologous chromosome sets in each cell, meaning humans are diploid organisms.

HOPE THIS HELPS

state two features of diffusion that do no apply to active transport​

Answers

diffusion moves ions from high concentration to low using no metabolic energy and active transport uses metabolic energy in the form of atp to move ions from high concentration to low concentration

What does active transport require that diffusion does not? Passive mechanisms like diffusion use no energy, while active transport requires energy to get done.

What two features distinguish active transport from diffusion? An example of diffusion is oxygen moving from the airways to the lungs - there is very little oxygen in the lungs but lots in the air. Active transport is the movement of particles from an area of low concentration to an area of high concentration. This process requires energy (ATP)

What are the 2 key features of active transport? There are two types of active transport: primary active transport that uses adenosine triphosphate (ATP), and secondary active transport that uses an electrochemical gradient. An example of active transport in human physiology is the uptake of glucose in the intestines.
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y= 4/3x -8
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Answers

Answer:

(x,y)= (x, 4/3x-8)

Explanation:

Help is needed please and thank you

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Answer

out of the cell

Answer: Into the cell

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In a point mutation, only one nucleotide is changed.

In a chromosomal mutation, the whole chromosome is changed.

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Substitution: ATT ACG ATA

Insertion: ATA TGC GAT A

how many sperms are formed in four primary spermatocyte​

Answers

One primary spermatocyte produces four spermatozoa, therefore, four primary spermatocytes will produce 16 spermatozoa.
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Which experiment would most likely contain experimental bias?

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Answer:

A company that makes pain relief medication tests the effectiveness of their own medicine compared to that of three other brands.

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Answers

Answer:

Yes you are right

Explanation:

I did it in test

Giving a lot of point and brainiest!!

How does cooling of magma affect crystal size?

A) Slow cooling creates small crystals

B) Fast cooling creates both large and small crystals.

C) Slow cooling creates large crystals

D) Fast cooling create large crystals

Answers

Answer:

When magma cools, crystals form because the solution is super-saturated with respect to some minerals. If the magma cools quickly, the crystals do not have much time to form, so they are very small. If the magma cools slowly, then the crystals have enough time to grow and become large.

Answer:

I learned about this, Uhm...

C is the right answer bc igneous rocks cool and form crystals.

Lava, since it comes out of a volcano, cools faster and has smaller crystals since they could not be developed enough.

Magma, still underground, cools slower than lava because of all the density and heat, which causes bigger crystals.

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How many atoms of the element hydrogen are there for each atom of oxygen in the compound we know of as 'water'?
a. 8
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c. 1
d. 2

Answers

Answer:

d

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Explanation:

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